Analysis of the Cellular Infiltrate in the Iris During Experimental Autoimmune Encephalomyelitis

A.F. De Vos, A.D. Dick, J. Kooster, L. Broersma, Paul Mcmenamin, A. Kijlstra

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

PURPOSE. Previous studies have shown that experimental autoimmune encephalomyelitis (EAE) and anterior uveitis (AU) develop in Lewis rats immunized with myelin basic protein (MBP). The purpose of this study was to characterize the dynamics, distribution, and phenotype of infiltrating cells in the iris during EAE-associated AU.METHODS. Lewis rats were immunized with MBP emulsified in complete Freund's adjuvant (CFA) or with CFA alone. Cellular infiltration of the iris was analyzed at various time points by immunohistochemistry of wholemounts, flow cytometry, and immunoelectron microscopy, by using monoclonal antibodies specific for monocytes/macrophages (ED1), T lymphocytes (R73, W3.25, OX8), T-cell activation markers (OX39, OX40), granulocytes (HIS48), major histocompatibility complex (MHC) class II (OX6), and neurofilament (2H3).RESULTS. MBP-immunized rats showed development of characteristic monophasic EAE, followed, after resolution of paralysis, by mild self-limited AU. Initially, focal infiltrates of round MHC class II+ and ED1(+) cells were found in the iris. During the course of AU, the midiris became massively infiltrated with ED1(+) monocytes-macrophages, R73(+) T cells, granulocytes (HIS48(+)), and MHC class II+ cells. The influx of T cells consisted of CD4(+) and CD8(+) cells, of which only a small fraction (
Original languageEnglish
Pages (from-to)3001-3010
JournalInvestigative ophthalmology & visual science
Volume41
Issue number10
Publication statusPublished - 2000

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