Analysis of tandem E-box motifs within human Complement receptor 2 (CR2/CD21) promoter reveals cell specific roles for RP58, E2A, USF and localized chromatin accessibility

Mark Cruickshank, James Dods, Rhonda Taylor, Mahdad Karimi, Emily Fenwick, Elizabeth Quail, Alex Rea, V.M. Holers, Lawrence Abraham, Daniela Ulgiati

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)
511 Downloads (Pure)


©2015 Elsevier Ltd. All rights reserved. Complement receptor 2 (CR2/CD21) plays an important role in the generation of normal B cell immune responses. As transcription appears to be the prime mechanism via which surface CR2/CD21 expression is controlled, understanding transcriptional regulation of this gene will have broader implications to B cell biology. Here we report opposing, cell-context specific control of CR2/CD21 promoter activity by tandem E-box elements, spaced 22 bp apart and within 70 bp of the transcription initiation site. We have identified E2A and USF transcription factors as binding to the distal and proximal E-box sites respectively in CR2-positive B-cells, at a site that is hypersensitive to restriction enzyme digestion compared to non-expressing K562 cells. However, additional unidentified proteins have also been found to bind these functionally important elements. By utilizing a proteomics approach we have identified a repressor protein, RP58, binding the distal E-box motif. Co-transfection experiments using RP58 overexpression constructs demonstrated a specific 10-fold repression of CR2/CD21 transcriptional activity mediated through the distal E-box repressor element. Taken together, our results indicate that repression of the CR2/CD21 promoter can occur through one of the E-box motifs via recruitment of RP58 and other factors to bring about a silenced chromatin context within CR2/CD21 non-expressing cells.
Original languageEnglish
Pages (from-to)107-119
Number of pages13
JournalInternational Journal of Biochemistry and Cell Biology
Early online date27 Mar 2015
Publication statusPublished - Jul 2015


Dive into the research topics of 'Analysis of tandem E-box motifs within human <em>Complement receptor 2</em> (<em>CR2/CD21</em>) promoter reveals cell specific roles for RP58, E2A, USF and localized chromatin accessibility'. Together they form a unique fingerprint.

Cite this