Analysis of post-transcriptional and architectural regulatory mechanisms controlling complement receptor gene expression in B cells

Joshua Clayton

doi:10.26182/5b85f40d48b12

Analysis of post-transcriptional and architectural regulatory mechanisms controlling complement receptor gene expression in B cells

Joshua Clayton

UWA Centre for Medical Research (affiliated with the Harry Perkins Institute of Medical Research)

Research output: Thesis › Doctoral Thesis

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Abstract

The regulators of complement activation (RCA) cluster on human chromosome 1 contains several functionally related genes whose products play crucial roles in the regulation of complement-mediated immune pathways. Many of these genes, such as complement receptor 2 (CR2), have been associated with the autoimmune disease systemic lupus erythematosus (SLE). We used chromosome conformation capture techniques to identify genomic interactions across the RCA gene cluster and characterised potential enhancers for CR2 in B cells. We also identified CR2 as a microRNA target and characterised the regulation and expression of several microRNAs important in B cell development.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Ulgiati, Daniela, Supervisor Abraham, Lawrie, Supervisor
Thesis sponsors	Australian Government Research Training Program
Award date	13 Aug 2018
DOIs	https://doi.org/10.26182/5b85f40d48b12
Publication status	Unpublished - 2018

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10.26182/5b85f40d48b12

THESIS - DOCTOR OF PHILOSOPHY - CLAYTON Joshua Steven - 2018
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Cite this

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abstract = "The regulators of complement activation (RCA) cluster on human chromosome 1 contains several functionally related genes whose products play crucial roles in the regulation of complement-mediated immune pathways. Many of these genes, such as complement receptor 2 (CR2), have been associated with the autoimmune disease systemic lupus erythematosus (SLE). We used chromosome conformation capture techniques to identify genomic interactions across the RCA gene cluster and characterised potential enhancers for CR2 in B cells. We also identified CR2 as a microRNA target and characterised the regulation and expression of several microRNAs important in B cell development.",

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N2 - The regulators of complement activation (RCA) cluster on human chromosome 1 contains several functionally related genes whose products play crucial roles in the regulation of complement-mediated immune pathways. Many of these genes, such as complement receptor 2 (CR2), have been associated with the autoimmune disease systemic lupus erythematosus (SLE). We used chromosome conformation capture techniques to identify genomic interactions across the RCA gene cluster and characterised potential enhancers for CR2 in B cells. We also identified CR2 as a microRNA target and characterised the regulation and expression of several microRNAs important in B cell development.

AB - The regulators of complement activation (RCA) cluster on human chromosome 1 contains several functionally related genes whose products play crucial roles in the regulation of complement-mediated immune pathways. Many of these genes, such as complement receptor 2 (CR2), have been associated with the autoimmune disease systemic lupus erythematosus (SLE). We used chromosome conformation capture techniques to identify genomic interactions across the RCA gene cluster and characterised potential enhancers for CR2 in B cells. We also identified CR2 as a microRNA target and characterised the regulation and expression of several microRNAs important in B cell development.

KW - B cells

KW - Enhancers

KW - MicroRNAs

KW - 3' UTR

KW - Regulators of complement

KW - Chromatin accessibility

KW - Complement receptor 2

KW - Topological domains

U2 - 10.26182/5b85f40d48b12

DO - 10.26182/5b85f40d48b12

M3 - Doctoral Thesis

ER -

Analysis of post-transcriptional and architectural regulatory mechanisms controlling complement receptor gene expression in B cells

Abstract

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