Analysis of p53 Gene Mutation by Polymerase chain reaction-single strand conformation Polymorphism provides independent prognostic information in Node-negative Breast Cancer

Barry Iacopetta, F. Grieu, Brenda Powell, R. Soong, K. Mccaul, R. Seshadri

Research output: Contribution to journalArticlepeer-review

40 Citations (Web of Science)

Abstract

Controversy continues regarding the prognostic utility of detection of p53 gene abnormalities in node-negative breast cancer. To resolve this, we used a rapid and nonisotopic PCR single strand conformation polymorphism method to screen for mutations in exons 4-8 of the p53 gene in primary tumors from 422 node-negative breast cancer patients. The prevalence of p53 mutation in the exons tested was 18%, p53 mutation was significantly associated with several markers of poor prognosis including larger tumor size, high tumor grade, low hormone receptor content, increased expression of MIB-1 (Ki-67), amplification of the HER-2/neu oncogene, and accumulation of the p53 protein. After a median duration follow-up period of 74 months, the parameters of tumor diameter greater than or equal to 20 mm, HER-2/neu oncogene amplification, and p53 mutation were found to be associated with a statistically significant shortened duration of disease-free and overall survival, but not the parameters of tumor grade, hormone receptor levels, or p53 expression, The poor prognosis associated with p53 mutation was observed primarily in patients with a tumor diameter of greater than or equal to 20 mm, In multivariate analysis, p53 mutation was a risk factor for increased risk of recurrence and death from breast cancer independent of tumor size, hormone receptor levels, HER-2/neu amplification, and MIB-1 expression. We conclude that a relatively simple and rapid single strand conformation polymorphism method of determining p53 mutation status in node-negative breast cancers can provide independent prognostic information.
Original languageEnglish
Pages (from-to)1597-1602
JournalClinical Cancer Research
Volume4
Issue number7
Publication statusPublished - 1998

Fingerprint

Dive into the research topics of 'Analysis of p53 Gene Mutation by Polymerase chain reaction-single strand conformation Polymorphism provides independent prognostic information in Node-negative Breast Cancer'. Together they form a unique fingerprint.

Cite this