Analysis of Nicotiana tabacum PIN genes identifies NtPIN4 as a key regulator of axillary bud growth

Xiaodong Xie, Guangyong Qin, Ping Si, Zhaopeng Luo, Junping Gao, Xia Chen, Jianfeng Zhang, Pan Wei, Qingyou Xia, Fucheng Lin, Jun Bo Yang

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    The plant-specific PIN-FORMED (PIN) auxin efflux proteins have been well characterized in many plant species, where they are crucial in the regulation of auxin transport in various aspects of plant development. However, little is known about the exact roles of the PIN genes during plant development in Nicotiana species. This study investigated the PIN genes in tobacco (Nicotiana tabacum) and in two ancestral species (Nicotiana sylvestris and Nicotiana tomentosiformis). Genome-wide analysis of the N. tabacum genome identified 20 genes of the PIN family. An in-depth phylogenetic analysis of the PIN genes of N. tabacum, N. sylvestris and N. tomentosiformis was conducted. NtPIN4 expression was strongly induced by the application of exogenous indole-3-acetic acid (IAA), but was downregulated by the application of ABA, a strigolactone analogue, and cytokinin, as well as by decapitation treatments, suggesting that the NtPIN4 expression level is likely positively regulated by auxin. Expression analysis indicated that NtPIN4 was highly expressed in tobacco stems and shoots, which was further validated through analysis of the activity of the NtPIN4 promoter. We used CRISPR-Cas9 technology to generate mutants for NtPIN4 and observed that both T0 and T1 plants had a significantly increased axillary bud growth phenotype, as compared with the wild-type plants. Therefore, NtPIN4 offers an opportunity for studying auxin-dependent branching processes.

    Original languageEnglish
    Pages (from-to)222-239
    Number of pages18
    JournalPhysiologia Plantarum
    Volume160
    Issue number2
    DOIs
    Publication statusPublished - 12 May 2017

    Fingerprint

    Nicotiana tabacum
    Tobacco
    auxins
    Nicotiana tomentosiformis
    buds
    Nicotiana sylvestris
    Indoleacetic Acids
    Growth
    Genes
    plant development
    tobacco
    genes
    decapitation
    Plant Development
    genome
    Nicotiana
    indole acetic acid
    cytokinins
    abscisic acid
    branching

    Cite this

    Xie, Xiaodong ; Qin, Guangyong ; Si, Ping ; Luo, Zhaopeng ; Gao, Junping ; Chen, Xia ; Zhang, Jianfeng ; Wei, Pan ; Xia, Qingyou ; Lin, Fucheng ; Yang, Jun Bo. / Analysis of Nicotiana tabacum PIN genes identifies NtPIN4 as a key regulator of axillary bud growth. In: Physiologia Plantarum. 2017 ; Vol. 160, No. 2. pp. 222-239.
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    title = "Analysis of Nicotiana tabacum PIN genes identifies NtPIN4 as a key regulator of axillary bud growth",
    abstract = "The plant-specific PIN-FORMED (PIN) auxin efflux proteins have been well characterized in many plant species, where they are crucial in the regulation of auxin transport in various aspects of plant development. However, little is known about the exact roles of the PIN genes during plant development in Nicotiana species. This study investigated the PIN genes in tobacco (Nicotiana tabacum) and in two ancestral species (Nicotiana sylvestris and Nicotiana tomentosiformis). Genome-wide analysis of the N. tabacum genome identified 20 genes of the PIN family. An in-depth phylogenetic analysis of the PIN genes of N. tabacum, N. sylvestris and N. tomentosiformis was conducted. NtPIN4 expression was strongly induced by the application of exogenous indole-3-acetic acid (IAA), but was downregulated by the application of ABA, a strigolactone analogue, and cytokinin, as well as by decapitation treatments, suggesting that the NtPIN4 expression level is likely positively regulated by auxin. Expression analysis indicated that NtPIN4 was highly expressed in tobacco stems and shoots, which was further validated through analysis of the activity of the NtPIN4 promoter. We used CRISPR-Cas9 technology to generate mutants for NtPIN4 and observed that both T0 and T1 plants had a significantly increased axillary bud growth phenotype, as compared with the wild-type plants. Therefore, NtPIN4 offers an opportunity for studying auxin-dependent branching processes.",
    author = "Xiaodong Xie and Guangyong Qin and Ping Si and Zhaopeng Luo and Junping Gao and Xia Chen and Jianfeng Zhang and Pan Wei and Qingyou Xia and Fucheng Lin and Yang, {Jun Bo}",
    year = "2017",
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    Xie, X, Qin, G, Si, P, Luo, Z, Gao, J, Chen, X, Zhang, J, Wei, P, Xia, Q, Lin, F & Yang, JB 2017, 'Analysis of Nicotiana tabacum PIN genes identifies NtPIN4 as a key regulator of axillary bud growth' Physiologia Plantarum, vol. 160, no. 2, pp. 222-239. https://doi.org/10.1111/ppl.12547

    Analysis of Nicotiana tabacum PIN genes identifies NtPIN4 as a key regulator of axillary bud growth. / Xie, Xiaodong; Qin, Guangyong; Si, Ping; Luo, Zhaopeng; Gao, Junping; Chen, Xia; Zhang, Jianfeng; Wei, Pan; Xia, Qingyou; Lin, Fucheng; Yang, Jun Bo.

    In: Physiologia Plantarum, Vol. 160, No. 2, 12.05.2017, p. 222-239.

    Research output: Contribution to journalArticle

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    AU - Xie, Xiaodong

    AU - Qin, Guangyong

    AU - Si, Ping

    AU - Luo, Zhaopeng

    AU - Gao, Junping

    AU - Chen, Xia

    AU - Zhang, Jianfeng

    AU - Wei, Pan

    AU - Xia, Qingyou

    AU - Lin, Fucheng

    AU - Yang, Jun Bo

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