Analysis of genes regulated by the transcription factor LUMAN identifies ApoA4 as a target gene in dendritic cells

Anna Sanecka; Marleen Ansems; Maaike A van Hout-Kuijer; Maaike W G Looman; Amy C Prosser; Suzanne Welten; Christian Gilissen; Iziah E Sama; Martijn A Huynen; Joris A Veltman; Bastiaan J H Jansen; Dagmar Eleveld-Trancikova; Gosse J Adema

doi:10.1016/j.molimm.2011.12.003

Analysis of genes regulated by the transcription factor LUMAN identifies ApoA4 as a target gene in dendritic cells

Anna Sanecka, Marleen Ansems, Maaike A van Hout-Kuijer, Maaike W G Looman, Amy C Prosser, Suzanne Welten, Christian Gilissen, Iziah E Sama, Martijn A Huynen, Joris A Veltman, Bastiaan J H Jansen, Dagmar Eleveld-Trancikova, Gosse J Adema

Research output: Contribution to journal › Article › peer-review

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Abstract

Dendritic cells (DCs) are professional antigen presenting cells of the immune system that play a crucial role in initiating immune responses and maintaining self tolerance. Better understanding of the molecular basis of DC immunobiology is required to improve DC-based immunotherapies. We previously described the interaction of transcription factor LUMAN (also known as CREB3 or LZIP) with the DC-specific transmembrane protein DC-STAMP in DCs. Target genes of LUMAN and its role in DCs are currently unknown. In this study we set out to identify genes regulated by LUMAN in DCs using microarray analysis. Expression of a constitutively active form of LUMAN in mouse DC cell line D2SC/1 identified Apolipoprotein A4 (ApoA4) as its target gene. Subsequent validation experiments, bioinformatics-based promoter analysis, and silencing studies confirmed that ApoA4 is a true target gene of LUMAN in bone marrow-derived DCs (BMDCs).

Original language	English
Pages (from-to)	66-73
Number of pages	8
Journal	Molecular Immunology
Volume	50
Issue number	1-2
DOIs	https://doi.org/10.1016/j.molimm.2011.12.003
Publication status	Published - Feb 2012
Externally published	Yes

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Sanecka, A., Ansems, M., van Hout-Kuijer, M. A., Looman, M. W. G., Prosser, A. C., Welten, S., Gilissen, C., Sama, I. E., Huynen, M. A., Veltman, J. A., Jansen, B. J. H., Eleveld-Trancikova, D., & Adema, G. J. (2012). Analysis of genes regulated by the transcription factor LUMAN identifies ApoA4 as a target gene in dendritic cells. Molecular Immunology, 50(1-2), 66-73. https://doi.org/10.1016/j.molimm.2011.12.003

@article{6aed093dbcbc4bebacf091aa890810ca,

title = "Analysis of genes regulated by the transcription factor LUMAN identifies ApoA4 as a target gene in dendritic cells",

abstract = "Dendritic cells (DCs) are professional antigen presenting cells of the immune system that play a crucial role in initiating immune responses and maintaining self tolerance. Better understanding of the molecular basis of DC immunobiology is required to improve DC-based immunotherapies. We previously described the interaction of transcription factor LUMAN (also known as CREB3 or LZIP) with the DC-specific transmembrane protein DC-STAMP in DCs. Target genes of LUMAN and its role in DCs are currently unknown. In this study we set out to identify genes regulated by LUMAN in DCs using microarray analysis. Expression of a constitutively active form of LUMAN in mouse DC cell line D2SC/1 identified Apolipoprotein A4 (ApoA4) as its target gene. Subsequent validation experiments, bioinformatics-based promoter analysis, and silencing studies confirmed that ApoA4 is a true target gene of LUMAN in bone marrow-derived DCs (BMDCs).",

keywords = "Animals, Apolipoproteins A/genetics, Bone Marrow Cells/drug effects, Brefeldin A/pharmacology, Cell Line, Cells, Cultured, Cyclic AMP Response Element-Binding Protein/genetics, Dendritic Cells/drug effects, Female, Gene Expression Profiling, Gene Expression Regulation, HEK293 Cells, Humans, Mice, Mice, Inbred C57BL, NIH 3T3 Cells, Oligonucleotide Array Sequence Analysis, Protein Synthesis Inhibitors/pharmacology, RNA Interference, Reverse Transcriptase Polymerase Chain Reaction",

author = "Anna Sanecka and Marleen Ansems and \{van Hout-Kuijer\}, \{Maaike A\} and Looman, \{Maaike W G\} and Prosser, \{Amy C\} and Suzanne Welten and Christian Gilissen and Sama, \{Iziah E\} and Huynen, \{Martijn A\} and Veltman, \{Joris A\} and Jansen, \{Bastiaan J H\} and Dagmar Eleveld-Trancikova and Adema, \{Gosse J\}",

year = "2012",

month = feb,

doi = "10.1016/j.molimm.2011.12.003",

language = "English",

volume = "50",

pages = "66--73",

journal = "Molecular Immunology",

issn = "0161-5890",

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Sanecka, A, Ansems, M, van Hout-Kuijer, MA, Looman, MWG, Prosser, AC, Welten, S, Gilissen, C, Sama, IE, Huynen, MA, Veltman, JA, Jansen, BJH, Eleveld-Trancikova, D & Adema, GJ 2012, 'Analysis of genes regulated by the transcription factor LUMAN identifies ApoA4 as a target gene in dendritic cells', Molecular Immunology, vol. 50, no. 1-2, pp. 66-73. https://doi.org/10.1016/j.molimm.2011.12.003

TY - JOUR

T1 - Analysis of genes regulated by the transcription factor LUMAN identifies ApoA4 as a target gene in dendritic cells

AU - Sanecka, Anna

AU - Ansems, Marleen

AU - van Hout-Kuijer, Maaike A

AU - Looman, Maaike W G

AU - Prosser, Amy C

AU - Welten, Suzanne

AU - Gilissen, Christian

AU - Sama, Iziah E

AU - Huynen, Martijn A

AU - Veltman, Joris A

AU - Jansen, Bastiaan J H

AU - Eleveld-Trancikova, Dagmar

AU - Adema, Gosse J

PY - 2012/2

Y1 - 2012/2

N2 - Dendritic cells (DCs) are professional antigen presenting cells of the immune system that play a crucial role in initiating immune responses and maintaining self tolerance. Better understanding of the molecular basis of DC immunobiology is required to improve DC-based immunotherapies. We previously described the interaction of transcription factor LUMAN (also known as CREB3 or LZIP) with the DC-specific transmembrane protein DC-STAMP in DCs. Target genes of LUMAN and its role in DCs are currently unknown. In this study we set out to identify genes regulated by LUMAN in DCs using microarray analysis. Expression of a constitutively active form of LUMAN in mouse DC cell line D2SC/1 identified Apolipoprotein A4 (ApoA4) as its target gene. Subsequent validation experiments, bioinformatics-based promoter analysis, and silencing studies confirmed that ApoA4 is a true target gene of LUMAN in bone marrow-derived DCs (BMDCs).

AB - Dendritic cells (DCs) are professional antigen presenting cells of the immune system that play a crucial role in initiating immune responses and maintaining self tolerance. Better understanding of the molecular basis of DC immunobiology is required to improve DC-based immunotherapies. We previously described the interaction of transcription factor LUMAN (also known as CREB3 or LZIP) with the DC-specific transmembrane protein DC-STAMP in DCs. Target genes of LUMAN and its role in DCs are currently unknown. In this study we set out to identify genes regulated by LUMAN in DCs using microarray analysis. Expression of a constitutively active form of LUMAN in mouse DC cell line D2SC/1 identified Apolipoprotein A4 (ApoA4) as its target gene. Subsequent validation experiments, bioinformatics-based promoter analysis, and silencing studies confirmed that ApoA4 is a true target gene of LUMAN in bone marrow-derived DCs (BMDCs).

KW - Animals

KW - Apolipoproteins A/genetics

KW - Bone Marrow Cells/drug effects

KW - Brefeldin A/pharmacology

KW - Cell Line

KW - Cells, Cultured

KW - Cyclic AMP Response Element-Binding Protein/genetics

KW - Dendritic Cells/drug effects

KW - Female

KW - Gene Expression Profiling

KW - Gene Expression Regulation

KW - HEK293 Cells

KW - Humans

KW - Mice

KW - Mice, Inbred C57BL

KW - NIH 3T3 Cells

KW - Oligonucleotide Array Sequence Analysis

KW - Protein Synthesis Inhibitors/pharmacology

KW - RNA Interference

KW - Reverse Transcriptase Polymerase Chain Reaction

UR - https://www.scopus.com/pages/publications/84857046342

U2 - 10.1016/j.molimm.2011.12.003

DO - 10.1016/j.molimm.2011.12.003

M3 - Article

C2 - 22209087

SN - 0161-5890

VL - 50

SP - 66

EP - 73

JO - Molecular Immunology

JF - Molecular Immunology

IS - 1-2

ER -

Analysis of genes regulated by the transcription factor LUMAN identifies ApoA4 as a target gene in dendritic cells

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