Analysis of Cytotoxic Effects of Zerumbone in Malignant Glioblastoma Cells

Mohammad Jalili-Nik; Amir R. Afshari; Khadijeh Mahboobnia; Paul C. Guest; Tannaz Jamialahmadi; Amirhossein Sahebkar

doi:10.1007/978-1-0716-1558-4_26

Analysis of Cytotoxic Effects of Zerumbone in Malignant Glioblastoma Cells

Mohammad Jalili-Nik
, Amir R. Afshari
, Khadijeh Mahboobnia
, Paul C. Guest
, Tannaz Jamialahmadi
, Amirhossein Sahebkar

Research output: Chapter in Book/Conference paper › Chapter › peer-review

7 Citations (Scopus)

Abstract

Glioblastoma multiforme (GBM) is an aggressive tumor in the central nervous system with a poor prognosis. Currently, the main interventions include surgery, chemotherapy, and radiotherapy. Recently, several natural products have been reported as potentially effective and safer treatment options. Here, we studied the effects of zerumbone, a sesquiterpene compound derived from Zingiber zerumbet Smith rhizomes, on human GBM U-87 MG cells in vitro. To meet this purpose, we used a cytotoxicity assay, as well as a quantitative polymerase chain reaction of apoptosis-related genes and western blot analysis of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), a transcription factor that controls the production of cytokines and molecules involved in cell survival.

Original language	English
Title of host publication	Physical Exercise and Natural and Synthetic Products in Health and Disease
Editors	Paul C. Guest
Publisher	Humana Press Inc.
Pages	361-369
Number of pages	9
Volume	2343
Edition	2022
ISBN (Electronic)	978-1-0716-1558-4
ISBN (Print)	978-1-0716-1557-7
DOIs	https://doi.org/10.1007/978-1-0716-1558-4_26
Publication status	Published - 2022
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	2343
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Access to Document

10.1007/978-1-0716-1558-4_26

Cite this

Jalili-Nik, M., Afshari, A. R., Mahboobnia, K., Guest, P. C., Jamialahmadi, T., & Sahebkar, A. (2022). Analysis of Cytotoxic Effects of Zerumbone in Malignant Glioblastoma Cells. In P. C. Guest (Ed.), Physical Exercise and Natural and Synthetic Products in Health and Disease (2022 ed., Vol. 2343, pp. 361-369). (Methods in Molecular Biology; Vol. 2343). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-1558-4_26

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abstract = "Glioblastoma multiforme (GBM) is an aggressive tumor in the central nervous system with a poor prognosis. Currently, the main interventions include surgery, chemotherapy, and radiotherapy. Recently, several natural products have been reported as potentially effective and safer treatment options. Here, we studied the effects of zerumbone, a sesquiterpene compound derived from Zingiber zerumbet Smith rhizomes, on human GBM U-87 MG cells in vitro. To meet this purpose, we used a cytotoxicity assay, as well as a quantitative polymerase chain reaction of apoptosis-related genes and western blot analysis of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), a transcription factor that controls the production of cytokines and molecules involved in cell survival.",

keywords = "Cytotoxicity, Glioblastoma multiforme, Natural products, qPCR, Western blot, Zerumbone",

author = "Mohammad Jalili-Nik and Afshari, \{Amir R.\} and Khadijeh Mahboobnia and Guest, \{Paul C.\} and Tannaz Jamialahmadi and Amirhossein Sahebkar",

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Jalili-Nik, M, Afshari, AR, Mahboobnia, K, Guest, PC, Jamialahmadi, T & Sahebkar, A 2022, Analysis of Cytotoxic Effects of Zerumbone in Malignant Glioblastoma Cells. in PC Guest (ed.), Physical Exercise and Natural and Synthetic Products in Health and Disease. 2022 edn, vol. 2343, Methods in Molecular Biology, vol. 2343, Humana Press Inc., pp. 361-369. https://doi.org/10.1007/978-1-0716-1558-4_26

Analysis of Cytotoxic Effects of Zerumbone in Malignant Glioblastoma Cells. / Jalili-Nik, Mohammad; Afshari, Amir R.; Mahboobnia, Khadijeh et al.
Physical Exercise and Natural and Synthetic Products in Health and Disease. ed. / Paul C. Guest. Vol. 2343 2022. ed. Humana Press Inc., 2022. p. 361-369 (Methods in Molecular Biology; Vol. 2343).

Research output: Chapter in Book/Conference paper › Chapter › peer-review

TY - CHAP

T1 - Analysis of Cytotoxic Effects of Zerumbone in Malignant Glioblastoma Cells

AU - Jalili-Nik, Mohammad

AU - Afshari, Amir R.

AU - Mahboobnia, Khadijeh

AU - Guest, Paul C.

AU - Jamialahmadi, Tannaz

AU - Sahebkar, Amirhossein

PY - 2022

Y1 - 2022

N2 - Glioblastoma multiforme (GBM) is an aggressive tumor in the central nervous system with a poor prognosis. Currently, the main interventions include surgery, chemotherapy, and radiotherapy. Recently, several natural products have been reported as potentially effective and safer treatment options. Here, we studied the effects of zerumbone, a sesquiterpene compound derived from Zingiber zerumbet Smith rhizomes, on human GBM U-87 MG cells in vitro. To meet this purpose, we used a cytotoxicity assay, as well as a quantitative polymerase chain reaction of apoptosis-related genes and western blot analysis of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), a transcription factor that controls the production of cytokines and molecules involved in cell survival.

AB - Glioblastoma multiforme (GBM) is an aggressive tumor in the central nervous system with a poor prognosis. Currently, the main interventions include surgery, chemotherapy, and radiotherapy. Recently, several natural products have been reported as potentially effective and safer treatment options. Here, we studied the effects of zerumbone, a sesquiterpene compound derived from Zingiber zerumbet Smith rhizomes, on human GBM U-87 MG cells in vitro. To meet this purpose, we used a cytotoxicity assay, as well as a quantitative polymerase chain reaction of apoptosis-related genes and western blot analysis of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), a transcription factor that controls the production of cytokines and molecules involved in cell survival.

KW - Cytotoxicity

KW - Glioblastoma multiforme

KW - Natural products

KW - qPCR

KW - Western blot

KW - Zerumbone

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U2 - 10.1007/978-1-0716-1558-4_26

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M3 - Chapter

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AN - SCOPUS:85114726226

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ER -

Jalili-Nik M, Afshari AR, Mahboobnia K, Guest PC, Jamialahmadi T, Sahebkar A. Analysis of Cytotoxic Effects of Zerumbone in Malignant Glioblastoma Cells. In Guest PC, editor, Physical Exercise and Natural and Synthetic Products in Health and Disease. 2022 ed. Vol. 2343. Humana Press Inc. 2022. p. 361-369. (Methods in Molecular Biology). doi: 10.1007/978-1-0716-1558-4_26

Analysis of Cytotoxic Effects of Zerumbone in Malignant Glioblastoma Cells

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