Analysis by proteomics reveals unique circulatory proteins in idiopathic pulmonary fibrosis

Yuben P. Moodley, Tamera J. Corte, Brian G. Oliver, Ian N. Glaspole, Andreja Livk, Jason Ito, Kirsten Peters, Richard Lipscombe, Tammy Casey, Dino B. A. Tan

Research output: Contribution to journalArticle

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Abstract

Background and objective Idiopathic pulmonary fibrosis (IPF) is a progressive fibrotic disease that has a poor 3-year median survival rate with unclear pathophysiology. Radiological features include bibasal, subpleural fibrosis and honeycombing while its pathology is characterized by fibroblastic foci and honeycombing. Proteomic analysis of circulating molecules in plasma may identify factors that characterize IPF and may assist in the diagnosis, prognostication and determination of pathogenic pathways in this condition. Methods Two independent quantitative proteomic techniques were used, isobaric tags for relative and absolute quantitation (iTRAQ) and multiple reaction monitoring (MRM), to identify differentially expressed plasma proteins in a group of IPF patients in comparison to healthy controls with normal lung function matched for age and gender. Results Five proteins were identified to be differentially expressed in IPF compared to healthy controls (upregulation of platelet basic protein and downregulation of actin, cytoplasmic 2, antithrombin-III, extracellular matrix protein-1 and fibronectin). Conclusion This study further validates the combinational use of non-targeted discovery proteomics (iTRAQ) with targeted quantitation by mass spectrometry (MRM) of soluble biomarkers to identify potentially important molecules and pathways for pulmonary diseases such as IPF.

Original languageEnglish
Number of pages4
JournalRespirology
DOIs
Publication statusE-pub ahead of print - 8 Aug 2019

Cite this

Moodley, Yuben P. ; Corte, Tamera J. ; Oliver, Brian G. ; Glaspole, Ian N. ; Livk, Andreja ; Ito, Jason ; Peters, Kirsten ; Lipscombe, Richard ; Casey, Tammy ; Tan, Dino B. A. / Analysis by proteomics reveals unique circulatory proteins in idiopathic pulmonary fibrosis. In: Respirology. 2019.
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abstract = "Background and objective Idiopathic pulmonary fibrosis (IPF) is a progressive fibrotic disease that has a poor 3-year median survival rate with unclear pathophysiology. Radiological features include bibasal, subpleural fibrosis and honeycombing while its pathology is characterized by fibroblastic foci and honeycombing. Proteomic analysis of circulating molecules in plasma may identify factors that characterize IPF and may assist in the diagnosis, prognostication and determination of pathogenic pathways in this condition. Methods Two independent quantitative proteomic techniques were used, isobaric tags for relative and absolute quantitation (iTRAQ) and multiple reaction monitoring (MRM), to identify differentially expressed plasma proteins in a group of IPF patients in comparison to healthy controls with normal lung function matched for age and gender. Results Five proteins were identified to be differentially expressed in IPF compared to healthy controls (upregulation of platelet basic protein and downregulation of actin, cytoplasmic 2, antithrombin-III, extracellular matrix protein-1 and fibronectin). Conclusion This study further validates the combinational use of non-targeted discovery proteomics (iTRAQ) with targeted quantitation by mass spectrometry (MRM) of soluble biomarkers to identify potentially important molecules and pathways for pulmonary diseases such as IPF.",
keywords = "idiopathic pulmonary fibrosis, isobaric tags for relative and absolute quantitation, multiple reaction monitoring, proteomics, PLATELET BASIC-PROTEIN, IPF",
author = "Moodley, {Yuben P.} and Corte, {Tamera J.} and Oliver, {Brian G.} and Glaspole, {Ian N.} and Andreja Livk and Jason Ito and Kirsten Peters and Richard Lipscombe and Tammy Casey and Tan, {Dino B. A.}",
year = "2019",
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Moodley, YP, Corte, TJ, Oliver, BG, Glaspole, IN, Livk, A, Ito, J, Peters, K, Lipscombe, R, Casey, T & Tan, DBA 2019, 'Analysis by proteomics reveals unique circulatory proteins in idiopathic pulmonary fibrosis' Respirology. https://doi.org/10.1111/resp.13668

Analysis by proteomics reveals unique circulatory proteins in idiopathic pulmonary fibrosis. / Moodley, Yuben P.; Corte, Tamera J.; Oliver, Brian G.; Glaspole, Ian N.; Livk, Andreja; Ito, Jason; Peters, Kirsten; Lipscombe, Richard; Casey, Tammy; Tan, Dino B. A.

In: Respirology, 08.08.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Analysis by proteomics reveals unique circulatory proteins in idiopathic pulmonary fibrosis

AU - Moodley, Yuben P.

AU - Corte, Tamera J.

AU - Oliver, Brian G.

AU - Glaspole, Ian N.

AU - Livk, Andreja

AU - Ito, Jason

AU - Peters, Kirsten

AU - Lipscombe, Richard

AU - Casey, Tammy

AU - Tan, Dino B. A.

PY - 2019/8/8

Y1 - 2019/8/8

N2 - Background and objective Idiopathic pulmonary fibrosis (IPF) is a progressive fibrotic disease that has a poor 3-year median survival rate with unclear pathophysiology. Radiological features include bibasal, subpleural fibrosis and honeycombing while its pathology is characterized by fibroblastic foci and honeycombing. Proteomic analysis of circulating molecules in plasma may identify factors that characterize IPF and may assist in the diagnosis, prognostication and determination of pathogenic pathways in this condition. Methods Two independent quantitative proteomic techniques were used, isobaric tags for relative and absolute quantitation (iTRAQ) and multiple reaction monitoring (MRM), to identify differentially expressed plasma proteins in a group of IPF patients in comparison to healthy controls with normal lung function matched for age and gender. Results Five proteins were identified to be differentially expressed in IPF compared to healthy controls (upregulation of platelet basic protein and downregulation of actin, cytoplasmic 2, antithrombin-III, extracellular matrix protein-1 and fibronectin). Conclusion This study further validates the combinational use of non-targeted discovery proteomics (iTRAQ) with targeted quantitation by mass spectrometry (MRM) of soluble biomarkers to identify potentially important molecules and pathways for pulmonary diseases such as IPF.

AB - Background and objective Idiopathic pulmonary fibrosis (IPF) is a progressive fibrotic disease that has a poor 3-year median survival rate with unclear pathophysiology. Radiological features include bibasal, subpleural fibrosis and honeycombing while its pathology is characterized by fibroblastic foci and honeycombing. Proteomic analysis of circulating molecules in plasma may identify factors that characterize IPF and may assist in the diagnosis, prognostication and determination of pathogenic pathways in this condition. Methods Two independent quantitative proteomic techniques were used, isobaric tags for relative and absolute quantitation (iTRAQ) and multiple reaction monitoring (MRM), to identify differentially expressed plasma proteins in a group of IPF patients in comparison to healthy controls with normal lung function matched for age and gender. Results Five proteins were identified to be differentially expressed in IPF compared to healthy controls (upregulation of platelet basic protein and downregulation of actin, cytoplasmic 2, antithrombin-III, extracellular matrix protein-1 and fibronectin). Conclusion This study further validates the combinational use of non-targeted discovery proteomics (iTRAQ) with targeted quantitation by mass spectrometry (MRM) of soluble biomarkers to identify potentially important molecules and pathways for pulmonary diseases such as IPF.

KW - idiopathic pulmonary fibrosis

KW - isobaric tags for relative and absolute quantitation

KW - multiple reaction monitoring

KW - proteomics

KW - PLATELET BASIC-PROTEIN

KW - IPF

U2 - 10.1111/resp.13668

DO - 10.1111/resp.13668

M3 - Article

JO - Asian Pacific Journal of Respirology

JF - Asian Pacific Journal of Respirology

SN - 1323-7799

ER -