Analyses of Indole Compounds in Sugar Cane (Saccharum officinarum L.) Juice by High Performance Liquid Chromatography and Liquid Chromatography-Mass Spectrometry after Solid-Phase Extraction

Jean Wan Hong Yong, Liya Ge, Wei San Wong, Zhen Ma, Swee Ngin Tan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Simultaneous quantitative analysis of 10 indole compounds, including indole-3-acetic acid (IAA, one of the most important naturally occurring auxins) and some of its metabolites, by high performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) after solid-phase extraction (SPE) was reported for the first time. The analysis was carried out using a reverse phase HPLC gradient elution, with an aqueous mobile phase (containing 0.1% formic acid) modified by methanol. Furthermore, a novel SPE procedure was developed for the pre-concentration and purification of indole compounds using C-18 SPE cartridges. The combination of SPE, HPLC, and LC-MS was applied to screen for the indole compounds present in sugar cane (Saccharum officinarum L.) juice, a refreshing beverage with various health benefits. Finally, four indole compounds were successfully detected and quantified in sugar cane juice by HPLC, which were further unequivocally confirmed by LC-MS/MS experiments operating in the multiple reaction monitoring (MRM) mode.

Original languageEnglish
Article number7
Number of pages12
JournalSeparations
Volume4
Issue number1
DOIs
Publication statusPublished - Mar 2017

Cite this

@article{3d5b61e5621d4cd0964e31dd4e080436,
title = "Analyses of Indole Compounds in Sugar Cane (Saccharum officinarum L.) Juice by High Performance Liquid Chromatography and Liquid Chromatography-Mass Spectrometry after Solid-Phase Extraction",
abstract = "Simultaneous quantitative analysis of 10 indole compounds, including indole-3-acetic acid (IAA, one of the most important naturally occurring auxins) and some of its metabolites, by high performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) after solid-phase extraction (SPE) was reported for the first time. The analysis was carried out using a reverse phase HPLC gradient elution, with an aqueous mobile phase (containing 0.1{\%} formic acid) modified by methanol. Furthermore, a novel SPE procedure was developed for the pre-concentration and purification of indole compounds using C-18 SPE cartridges. The combination of SPE, HPLC, and LC-MS was applied to screen for the indole compounds present in sugar cane (Saccharum officinarum L.) juice, a refreshing beverage with various health benefits. Finally, four indole compounds were successfully detected and quantified in sugar cane juice by HPLC, which were further unequivocally confirmed by LC-MS/MS experiments operating in the multiple reaction monitoring (MRM) mode.",
keywords = "indole compounds, high performance liquid chromatography, liquid chromatography-tandem mass spectrometry, solid-phase extraction, sugar cane juice, INDOLE-3-PYRUVIC ACID, ARABIDOPSIS-THALIANA, ANTIOXIDANT ACTIVITY, IN-VITRO, QUANTIFICATION, DERIVATIVES, INDOLE-3-ACETAMIDE, ELECTROPHORESIS, PHYTOHORMONES, BIOSYNTHESIS",
author = "Yong, {Jean Wan Hong} and Liya Ge and Wong, {Wei San} and Zhen Ma and Tan, {Swee Ngin}",
year = "2017",
month = "3",
doi = "10.3390/separations4010007",
language = "English",
volume = "4",
journal = "Separations",
issn = "2227-9075",
publisher = "MDPI AG",
number = "1",

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Analyses of Indole Compounds in Sugar Cane (Saccharum officinarum L.) Juice by High Performance Liquid Chromatography and Liquid Chromatography-Mass Spectrometry after Solid-Phase Extraction. / Yong, Jean Wan Hong; Ge, Liya; Wong, Wei San; Ma, Zhen; Tan, Swee Ngin.

In: Separations, Vol. 4, No. 1, 7, 03.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Analyses of Indole Compounds in Sugar Cane (Saccharum officinarum L.) Juice by High Performance Liquid Chromatography and Liquid Chromatography-Mass Spectrometry after Solid-Phase Extraction

AU - Yong, Jean Wan Hong

AU - Ge, Liya

AU - Wong, Wei San

AU - Ma, Zhen

AU - Tan, Swee Ngin

PY - 2017/3

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N2 - Simultaneous quantitative analysis of 10 indole compounds, including indole-3-acetic acid (IAA, one of the most important naturally occurring auxins) and some of its metabolites, by high performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) after solid-phase extraction (SPE) was reported for the first time. The analysis was carried out using a reverse phase HPLC gradient elution, with an aqueous mobile phase (containing 0.1% formic acid) modified by methanol. Furthermore, a novel SPE procedure was developed for the pre-concentration and purification of indole compounds using C-18 SPE cartridges. The combination of SPE, HPLC, and LC-MS was applied to screen for the indole compounds present in sugar cane (Saccharum officinarum L.) juice, a refreshing beverage with various health benefits. Finally, four indole compounds were successfully detected and quantified in sugar cane juice by HPLC, which were further unequivocally confirmed by LC-MS/MS experiments operating in the multiple reaction monitoring (MRM) mode.

AB - Simultaneous quantitative analysis of 10 indole compounds, including indole-3-acetic acid (IAA, one of the most important naturally occurring auxins) and some of its metabolites, by high performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) after solid-phase extraction (SPE) was reported for the first time. The analysis was carried out using a reverse phase HPLC gradient elution, with an aqueous mobile phase (containing 0.1% formic acid) modified by methanol. Furthermore, a novel SPE procedure was developed for the pre-concentration and purification of indole compounds using C-18 SPE cartridges. The combination of SPE, HPLC, and LC-MS was applied to screen for the indole compounds present in sugar cane (Saccharum officinarum L.) juice, a refreshing beverage with various health benefits. Finally, four indole compounds were successfully detected and quantified in sugar cane juice by HPLC, which were further unequivocally confirmed by LC-MS/MS experiments operating in the multiple reaction monitoring (MRM) mode.

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KW - liquid chromatography-tandem mass spectrometry

KW - solid-phase extraction

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KW - INDOLE-3-PYRUVIC ACID

KW - ARABIDOPSIS-THALIANA

KW - ANTIOXIDANT ACTIVITY

KW - IN-VITRO

KW - QUANTIFICATION

KW - DERIVATIVES

KW - INDOLE-3-ACETAMIDE

KW - ELECTROPHORESIS

KW - PHYTOHORMONES

KW - BIOSYNTHESIS

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