TY - JOUR
T1 - An isolated perfused human placental lobule model for multiple indicator dilution studies
AU - Rasiah, Rohan L.
AU - Addison, Russell S.
AU - Mortimer, Robin H.
PY - 1997/9/1
Y1 - 1997/9/1
N2 - We describe a method for multiple indicator dilution studies in the isolated perfused human placental lobule developed to investigate the relationships between changes in pressure and flow and solute clearance. A peripheral lobule of a human placenta is perfused with a tissue culture-based medium and the perfusate oxygen tension, arterial and venous pressures, pH and perfusion temperature continuously monitored by a computerized system. Flow rates are readily changed. Bolus injections of vascular, extracellular and water space markers, and study compounds can be made into either maternal or fetal circulations, and precisely timed outflow fractions can be collected with computer-controlled fraction collectors, allowing simultaneous determination of concentration-time profiles of each marker.
AB - We describe a method for multiple indicator dilution studies in the isolated perfused human placental lobule developed to investigate the relationships between changes in pressure and flow and solute clearance. A peripheral lobule of a human placenta is perfused with a tissue culture-based medium and the perfusate oxygen tension, arterial and venous pressures, pH and perfusion temperature continuously monitored by a computerized system. Flow rates are readily changed. Bolus injections of vascular, extracellular and water space markers, and study compounds can be made into either maternal or fetal circulations, and precisely timed outflow fractions can be collected with computer-controlled fraction collectors, allowing simultaneous determination of concentration-time profiles of each marker.
KW - Area under the curve
KW - Multiple indicator dilution
KW - Oxygen and pH electrodes
KW - Placental perfusion
KW - Pressure transducers
KW - Statistical moments
UR - http://www.scopus.com/inward/record.url?scp=0030729627&partnerID=8YFLogxK
U2 - 10.1016/S1056-8719(97)00032-4
DO - 10.1016/S1056-8719(97)00032-4
M3 - Article
C2 - 9339412
AN - SCOPUS:0030729627
SN - 1056-8719
VL - 38
SP - 19
EP - 25
JO - Journal of Pharmacological and Toxicological Methods
JF - Journal of Pharmacological and Toxicological Methods
IS - 1
ER -