An isolated perfused human placental lobule model for multiple indicator dilution studies

Rohan L. Rasiah, Russell S. Addison, Robin H. Mortimer

    Research output: Contribution to journalArticle

    2 Citations (Scopus)

    Abstract

    We describe a method for multiple indicator dilution studies in the isolated perfused human placental lobule developed to investigate the relationships between changes in pressure and flow and solute clearance. A peripheral lobule of a human placenta is perfused with a tissue culture-based medium and the perfusate oxygen tension, arterial and venous pressures, pH and perfusion temperature continuously monitored by a computerized system. Flow rates are readily changed. Bolus injections of vascular, extracellular and water space markers, and study compounds can be made into either maternal or fetal circulations, and precisely timed outflow fractions can be collected with computer-controlled fraction collectors, allowing simultaneous determination of concentration-time profiles of each marker.

    Original languageEnglish
    Pages (from-to)19-25
    Number of pages7
    JournalJournal of Pharmacological and Toxicological Methods
    Volume38
    Issue number1
    DOIs
    Publication statusPublished - 1 Sep 1997

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