An investigation onto Cd toxicity to freshwater microalga Chlorella sorokiniana in mixotrophy and photoautotrophy: A Bayesian approach

Adriano Evandir Marchello, Natalia Lombardi Oliveira, Ana Teresa Lombardi, Adriano Polpo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Aquatic ecosystems are composed by a myriad of dissolved organic materials that can be assimilated by microalgae, while they can perform photosynthesis, this is refereed as mixotrophy. However, ecotoxicological tests usually consider only the photoautotrophic metabolism. This research investigated the ecotoxicological differences between photoautotrophy and mixotrophy in Chlorella sorokiniana exposed to cadmium (Cd). Chlorophyll a, photosynthetic efficiency (Fv/Fm), cell viability, biochemical composition and pH were used to monitor possible toxic effects at 72 h cultures. Glucose (1 g.L-1) was used as organic carbon source. To evaluate the probability of the photoautotrophic culture being more affected by Cc than the mixotrophic one, Bayesian statistical analysis was performed. The photoautotrophic cultures were more affected by Cd than the mixotrophic ones, with reduction of all evaluated parameters, excep for protein concentration. However, in mixotrophic cultures, no changes in protein concentration and proteins:carbohydrates ratio were observed, and chlorophyll a, Fv/Fm and cell viability were only affected at the high Cd concentrations (range In -11.5 to -9.4). However, both mixotrophy and photoautotrophy had the same probability of having the carbohydrates concentration affected by Cd. We conclude that the microalgae in mixotrophy were more resistant to the Cd than in photoautotrophy. In addition, we showed that under photoautotrophy Fv/Fm decreased linearly as Cd concentration increased, but in mixotrophy no effect was observed up to 10-5 molL(-1) Cd, after which it decreased. We rationale that the reduced photosynthetic capacity under mixotrophy can end up reducing the release of oxygen gas, which can compromise the entire aquatic ecosystem. (C) 2018 Elsevier Ltd. All rights reserved

Original languageEnglish
Pages (from-to)794-803
Number of pages10
JournalChemosphere
Volume211
DOIs
Publication statusPublished - Nov 2018
Externally publishedYes

Cite this

Marchello, Adriano Evandir ; Oliveira, Natalia Lombardi ; Lombardi, Ana Teresa ; Polpo, Adriano. / An investigation onto Cd toxicity to freshwater microalga Chlorella sorokiniana in mixotrophy and photoautotrophy : A Bayesian approach. In: Chemosphere. 2018 ; Vol. 211. pp. 794-803.
@article{e1710d5065b441328a562957dd1a7ca4,
title = "An investigation onto Cd toxicity to freshwater microalga Chlorella sorokiniana in mixotrophy and photoautotrophy: A Bayesian approach",
abstract = "Aquatic ecosystems are composed by a myriad of dissolved organic materials that can be assimilated by microalgae, while they can perform photosynthesis, this is refereed as mixotrophy. However, ecotoxicological tests usually consider only the photoautotrophic metabolism. This research investigated the ecotoxicological differences between photoautotrophy and mixotrophy in Chlorella sorokiniana exposed to cadmium (Cd). Chlorophyll a, photosynthetic efficiency (Fv/Fm), cell viability, biochemical composition and pH were used to monitor possible toxic effects at 72 h cultures. Glucose (1 g.L-1) was used as organic carbon source. To evaluate the probability of the photoautotrophic culture being more affected by Cc than the mixotrophic one, Bayesian statistical analysis was performed. The photoautotrophic cultures were more affected by Cd than the mixotrophic ones, with reduction of all evaluated parameters, excep for protein concentration. However, in mixotrophic cultures, no changes in protein concentration and proteins:carbohydrates ratio were observed, and chlorophyll a, Fv/Fm and cell viability were only affected at the high Cd concentrations (range In -11.5 to -9.4). However, both mixotrophy and photoautotrophy had the same probability of having the carbohydrates concentration affected by Cd. We conclude that the microalgae in mixotrophy were more resistant to the Cd than in photoautotrophy. In addition, we showed that under photoautotrophy Fv/Fm decreased linearly as Cd concentration increased, but in mixotrophy no effect was observed up to 10-5 molL(-1) Cd, after which it decreased. We rationale that the reduced photosynthetic capacity under mixotrophy can end up reducing the release of oxygen gas, which can compromise the entire aquatic ecosystem. (C) 2018 Elsevier Ltd. All rights reserved",
keywords = "Mixotrophic, growth, Cd, Glucose, Biochemical composition, PHOTOSYNTHETIC RESPONSES, PHYTOCHELATIN PRODUCTION, NEOCHLORIS-OLEOABUNDANS, CADMIUM, COPPER, GROWTH, ACCUMULATION, VULGARIS, PROTEIN, STRESS",
author = "Marchello, {Adriano Evandir} and Oliveira, {Natalia Lombardi} and Lombardi, {Ana Teresa} and Adriano Polpo",
year = "2018",
month = "11",
doi = "10.1016/j.chemosphere.2018.08.019",
language = "English",
volume = "211",
pages = "794--803",
journal = "Chemosphere",
issn = "0045-6535",
publisher = "Pergamon",

}

An investigation onto Cd toxicity to freshwater microalga Chlorella sorokiniana in mixotrophy and photoautotrophy : A Bayesian approach. / Marchello, Adriano Evandir; Oliveira, Natalia Lombardi; Lombardi, Ana Teresa; Polpo, Adriano.

In: Chemosphere, Vol. 211, 11.2018, p. 794-803.

Research output: Contribution to journalArticle

TY - JOUR

T1 - An investigation onto Cd toxicity to freshwater microalga Chlorella sorokiniana in mixotrophy and photoautotrophy

T2 - A Bayesian approach

AU - Marchello, Adriano Evandir

AU - Oliveira, Natalia Lombardi

AU - Lombardi, Ana Teresa

AU - Polpo, Adriano

PY - 2018/11

Y1 - 2018/11

N2 - Aquatic ecosystems are composed by a myriad of dissolved organic materials that can be assimilated by microalgae, while they can perform photosynthesis, this is refereed as mixotrophy. However, ecotoxicological tests usually consider only the photoautotrophic metabolism. This research investigated the ecotoxicological differences between photoautotrophy and mixotrophy in Chlorella sorokiniana exposed to cadmium (Cd). Chlorophyll a, photosynthetic efficiency (Fv/Fm), cell viability, biochemical composition and pH were used to monitor possible toxic effects at 72 h cultures. Glucose (1 g.L-1) was used as organic carbon source. To evaluate the probability of the photoautotrophic culture being more affected by Cc than the mixotrophic one, Bayesian statistical analysis was performed. The photoautotrophic cultures were more affected by Cd than the mixotrophic ones, with reduction of all evaluated parameters, excep for protein concentration. However, in mixotrophic cultures, no changes in protein concentration and proteins:carbohydrates ratio were observed, and chlorophyll a, Fv/Fm and cell viability were only affected at the high Cd concentrations (range In -11.5 to -9.4). However, both mixotrophy and photoautotrophy had the same probability of having the carbohydrates concentration affected by Cd. We conclude that the microalgae in mixotrophy were more resistant to the Cd than in photoautotrophy. In addition, we showed that under photoautotrophy Fv/Fm decreased linearly as Cd concentration increased, but in mixotrophy no effect was observed up to 10-5 molL(-1) Cd, after which it decreased. We rationale that the reduced photosynthetic capacity under mixotrophy can end up reducing the release of oxygen gas, which can compromise the entire aquatic ecosystem. (C) 2018 Elsevier Ltd. All rights reserved

AB - Aquatic ecosystems are composed by a myriad of dissolved organic materials that can be assimilated by microalgae, while they can perform photosynthesis, this is refereed as mixotrophy. However, ecotoxicological tests usually consider only the photoautotrophic metabolism. This research investigated the ecotoxicological differences between photoautotrophy and mixotrophy in Chlorella sorokiniana exposed to cadmium (Cd). Chlorophyll a, photosynthetic efficiency (Fv/Fm), cell viability, biochemical composition and pH were used to monitor possible toxic effects at 72 h cultures. Glucose (1 g.L-1) was used as organic carbon source. To evaluate the probability of the photoautotrophic culture being more affected by Cc than the mixotrophic one, Bayesian statistical analysis was performed. The photoautotrophic cultures were more affected by Cd than the mixotrophic ones, with reduction of all evaluated parameters, excep for protein concentration. However, in mixotrophic cultures, no changes in protein concentration and proteins:carbohydrates ratio were observed, and chlorophyll a, Fv/Fm and cell viability were only affected at the high Cd concentrations (range In -11.5 to -9.4). However, both mixotrophy and photoautotrophy had the same probability of having the carbohydrates concentration affected by Cd. We conclude that the microalgae in mixotrophy were more resistant to the Cd than in photoautotrophy. In addition, we showed that under photoautotrophy Fv/Fm decreased linearly as Cd concentration increased, but in mixotrophy no effect was observed up to 10-5 molL(-1) Cd, after which it decreased. We rationale that the reduced photosynthetic capacity under mixotrophy can end up reducing the release of oxygen gas, which can compromise the entire aquatic ecosystem. (C) 2018 Elsevier Ltd. All rights reserved

KW - Mixotrophic

KW - growth

KW - Cd

KW - Glucose

KW - Biochemical composition

KW - PHOTOSYNTHETIC RESPONSES

KW - PHYTOCHELATIN PRODUCTION

KW - NEOCHLORIS-OLEOABUNDANS

KW - CADMIUM

KW - COPPER

KW - GROWTH

KW - ACCUMULATION

KW - VULGARIS

KW - PROTEIN

KW - STRESS

U2 - 10.1016/j.chemosphere.2018.08.019

DO - 10.1016/j.chemosphere.2018.08.019

M3 - Article

VL - 211

SP - 794

EP - 803

JO - Chemosphere

JF - Chemosphere

SN - 0045-6535

ER -