An investigation of the antioxidant activity and phenolic constituents in Western Australian honeybee products

Ivan Lawag

doi:10.26182/vak1-6368

An investigation of the antioxidant activity and phenolic constituents in Western Australian honeybee products

Ivan Lawag

Research output: Thesis › Doctoral Thesis

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Abstract

This study focuses on determination of total antioxidant activity (TAA), identification/quantification of phenolic compounds and the contribution of individual constituents to the TAA of WA honeybee products. It details the optimisation/validation of assays to determine total phenolic content and TAA of honeybee products and their application to the analysis of WA honeys and bee pollen. For the latter extraction protocol preceding analysis was also optimised. Based on a comprehensive review of phenolic honey constituents a novel HPTLC-derived database for their identification was developed/validated. So identified phenolics in WA honeys were quantified and those with antioxidant activity visualised using HPTLC-DPPH bioautography.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Locher, Connie, Supervisor Lim, Lee Yong, Supervisor Hammer, Kate, Supervisor
Thesis sponsors	Australian Government Research Training Program
Award date	11 Jul 2023
DOIs	https://doi.org/10.26182/vak1-6368
Publication status	Unpublished - 2023

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10.26182/vak1-6368

THESIS - DOCTOR OF PHILOSOPHY - LAWAG Ivan Lozada - 2023
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4 Article
1 Review article

A Modified Folin-Ciocalteu Assay for the Determination of Total Phenolics Content in Honey
Lawag, I., Nolden, E., Schaper, A., Lim, L. Y. & Locher, C., 7 Feb 2023, In: Applied Sciences (Switzerland). 13, 4, 17 p., 2135.
Research output: Contribution to journal › Article › peer-review

Open Access
24 Citations (Scopus)
Antioxidant Activity and Phenolic Compound Identification and Quantification in Western Australian Honeys
Lawag, I., Islam, M. K., Sostaric, T., Lim, L. Y., Hammer, K. & Locher, C., 12 Jan 2023, In: Antioxidants. 12, 1, 35 p., 189.
Research output: Contribution to journal › Article › peer-review

Open Access
21 Citations (Scopus)
A Comprehensive Survey of Phenolic Constituents Reported in Monofloral Honeys around the Globe
Lawag, I., Lim, L. Y., Joshi, R., Hammer, K. & Locher, C., 1 Apr 2022, In: Foods. 11, 8, p. 1-42 42 p., 1152.
Research output: Contribution to journal › Review article › peer-review

Open Access
17 Citations (Scopus)

Cite this

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title = "An investigation of the antioxidant activity and phenolic constituents in Western Australian honeybee products",

abstract = "This study focuses on determination of total antioxidant activity (TAA), identification/quantification of phenolic compounds and the contribution of individual constituents to the TAA of WA honeybee products. It details the optimisation/validation of assays to determine total phenolic content and TAA of honeybee products and their application to the analysis of WA honeys and bee pollen. For the latter extraction protocol preceding analysis was also optimised. Based on a comprehensive review of phenolic honey constituents a novel HPTLC-derived database for their identification was developed/validated. So identified phenolics in WA honeys were quantified and those with antioxidant activity visualised using HPTLC-DPPH bioautography.",

keywords = "honey, bee pollen, Modified-Folin-Ciocalteu, DPPH, FRAP, HPTLC, HPTLC-DPPH Assay, Phenolics",

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year = "2023",

doi = "10.26182/vak1-6368",

language = "English",

school = "The University of Western Australia",

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AU - Lawag, Ivan

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N2 - This study focuses on determination of total antioxidant activity (TAA), identification/quantification of phenolic compounds and the contribution of individual constituents to the TAA of WA honeybee products. It details the optimisation/validation of assays to determine total phenolic content and TAA of honeybee products and their application to the analysis of WA honeys and bee pollen. For the latter extraction protocol preceding analysis was also optimised. Based on a comprehensive review of phenolic honey constituents a novel HPTLC-derived database for their identification was developed/validated. So identified phenolics in WA honeys were quantified and those with antioxidant activity visualised using HPTLC-DPPH bioautography.

AB - This study focuses on determination of total antioxidant activity (TAA), identification/quantification of phenolic compounds and the contribution of individual constituents to the TAA of WA honeybee products. It details the optimisation/validation of assays to determine total phenolic content and TAA of honeybee products and their application to the analysis of WA honeys and bee pollen. For the latter extraction protocol preceding analysis was also optimised. Based on a comprehensive review of phenolic honey constituents a novel HPTLC-derived database for their identification was developed/validated. So identified phenolics in WA honeys were quantified and those with antioxidant activity visualised using HPTLC-DPPH bioautography.

KW - honey

KW - bee pollen

KW - Modified-Folin-Ciocalteu

KW - DPPH

KW - FRAP

KW - HPTLC

KW - HPTLC-DPPH Assay

KW - Phenolics

U2 - 10.26182/vak1-6368

DO - 10.26182/vak1-6368

M3 - Doctoral Thesis

ER -

An investigation of the antioxidant activity and phenolic constituents in Western Australian honeybee products

Abstract

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Research output

A Modified Folin-Ciocalteu Assay for the Determination of Total Phenolics Content in Honey

Antioxidant Activity and Phenolic Compound Identification and Quantification in Western Australian Honeys

A Comprehensive Survey of Phenolic Constituents Reported in Monofloral Honeys around the Globe

Cite this