An in vitro technique for electrophysiological mapping of reptilian retinotectal projections

R.V. Stirling, Sarah Dunlop, Lyn Beazley

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

An in vitro procedure is described for electrophysiological mapping of the retinotectal projections using an eye-cup and brain stem preparation which remains viable for up to 30 h. The technique has been found to be successful in turtles and lizards and may be useful for other species in which metabolism is greatly depressed by low temperatures. There are several advantages over in vivo recording, including the longevity and stability of the preparation, an absence of confounding anaesthetic effects and the ability to record from the retina as well as from the brain. The technique offers opportunities to introduce pharmacological agents via the perfusate or to conduct anatomical tracing studies coincident with electrophysiological recording. (C) 1998 Elsevier Science B.V. All rights reserved.
Original languageEnglish
Pages (from-to)85-89
JournalJournal of Neuroscience Methods
Volume81
DOIs
Publication statusPublished - 1998

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