An in vitro study of a novel quaternary ammonium silane endodontic irrigant

U. Daood, A. Parolia, A. Elkezza, C. K. Yiu, P. Abbott, J. P. Matinlinna, A. S. Fawzy

Research output: Contribution to journalArticle

Abstract

Objective: To analyze effect of NaOCl + 2% quaternary ammonium silane (QAS)-containing novel irrigant against bacteria impregnated inside the root canal system, and to evaluate its antimicrobial and mechanical potential of dentine substrate. Methods: Root canal was prepared using stainless steel K-files™ and ProTaper™ and subjected to manual and ultrasonic irrigation using 6% NaOCl + 2% CHX, 6% NaOCl + 2% QAS and saline as control. For confocal-microscopy, Raman spectroscopy and SEM analysis before and after treatment, Enterococcus faecalis cultured for 7 days. Raman spectroscopy analysis was done across cut section of gutta percha/sealer-dentine to detect resin infiltration. Indentation of mechanical properties was evaluated using a Berkovich indenter. The contact angle of irrigants and surface free energy were evaluated. Mineralization nodules were detected through Alazarin red after 14 days. Results: Control biofilms showed dense green colonies. Majority of E. faecalis bacteria were present in biofilm fluoresced red in NaOCl + 2% QAS group. There was reduction of 484 cm−1 Raman band and its intensity reached lowest with NaOCl + 2% QAS. There was an increase in 1350–1420 cm−1 intensity in the NaOCl + 2% CHX groups. Gradual decrease in 1639 cm−1 and 1609 cm−1 Raman signal ratios were seen in the resin-depth region of 17 μm>, 14.1 μm> and 13.2 μm for NaOCl + 2% QAS, NaOCl + 2% CHX and control groups respectively. All obturated groups showed an intact sealer/dentine interface with a few notable differences. 0.771 and 83.5% creep indentation distance for NaOCl + 2% QAS ultrasonic groups were observed. Highest proportion of polar component was significantly found in the NaOCl + 2% QAS groups which was significantly higher as compared to other groups. Mineralized nodules were increased in NaOCl + 2% QAS. Significance: Favorable antimicrobial and endodontic profile of the NaOCl + 2% QAS solution might suggest clinical use for it for more predictable reduction of intracanal bacteria.

Original languageEnglish
JournalDental Materials
DOIs
Publication statusE-pub ahead of print - 12 Jun 2019

Fingerprint

Silanes
Endodontics
Ammonium Compounds
Dentin
Bacteria
Raman Spectrum Analysis
Enterococcus faecalis
Dental Pulp Cavity
Biofilms
Canals
Indentation
Ultrasonics
Raman spectroscopy
Resins
Gutta-Percha
In Vitro Techniques
Confocal microscopy
Stainless Steel
Infiltration
Irrigation

Cite this

Daood, U. ; Parolia, A. ; Elkezza, A. ; Yiu, C. K. ; Abbott, P. ; Matinlinna, J. P. ; Fawzy, A. S. / An in vitro study of a novel quaternary ammonium silane endodontic irrigant. In: Dental Materials. 2019.
@article{eabba18c70214b9eb7e8fdbef99805b1,
title = "An in vitro study of a novel quaternary ammonium silane endodontic irrigant",
abstract = "Objective: To analyze effect of NaOCl + 2{\%} quaternary ammonium silane (QAS)-containing novel irrigant against bacteria impregnated inside the root canal system, and to evaluate its antimicrobial and mechanical potential of dentine substrate. Methods: Root canal was prepared using stainless steel K-files™ and ProTaper™ and subjected to manual and ultrasonic irrigation using 6{\%} NaOCl + 2{\%} CHX, 6{\%} NaOCl + 2{\%} QAS and saline as control. For confocal-microscopy, Raman spectroscopy and SEM analysis before and after treatment, Enterococcus faecalis cultured for 7 days. Raman spectroscopy analysis was done across cut section of gutta percha/sealer-dentine to detect resin infiltration. Indentation of mechanical properties was evaluated using a Berkovich indenter. The contact angle of irrigants and surface free energy were evaluated. Mineralization nodules were detected through Alazarin red after 14 days. Results: Control biofilms showed dense green colonies. Majority of E. faecalis bacteria were present in biofilm fluoresced red in NaOCl + 2{\%} QAS group. There was reduction of 484 cm−1 Raman band and its intensity reached lowest with NaOCl + 2{\%} QAS. There was an increase in 1350–1420 cm−1 intensity in the NaOCl + 2{\%} CHX groups. Gradual decrease in 1639 cm−1 and 1609 cm−1 Raman signal ratios were seen in the resin-depth region of 17 μm>, 14.1 μm> and 13.2 μm for NaOCl + 2{\%} QAS, NaOCl + 2{\%} CHX and control groups respectively. All obturated groups showed an intact sealer/dentine interface with a few notable differences. 0.771 and 83.5{\%} creep indentation distance for NaOCl + 2{\%} QAS ultrasonic groups were observed. Highest proportion of polar component was significantly found in the NaOCl + 2{\%} QAS groups which was significantly higher as compared to other groups. Mineralized nodules were increased in NaOCl + 2{\%} QAS. Significance: Favorable antimicrobial and endodontic profile of the NaOCl + 2{\%} QAS solution might suggest clinical use for it for more predictable reduction of intracanal bacteria.",
keywords = "Antimicrobial, Condensation, Confocal microscopy, E. faecalis, Quaternary ammonium silane, Raman spectroscopy, Root dentine",
author = "U. Daood and A. Parolia and A. Elkezza and Yiu, {C. K.} and P. Abbott and Matinlinna, {J. P.} and Fawzy, {A. S.}",
year = "2019",
month = "6",
day = "12",
doi = "10.1016/j.dental.2019.05.020",
language = "English",
journal = "Dental Materials",
issn = "0109-5641",
publisher = "Elsevier",

}

An in vitro study of a novel quaternary ammonium silane endodontic irrigant. / Daood, U.; Parolia, A.; Elkezza, A.; Yiu, C. K.; Abbott, P.; Matinlinna, J. P.; Fawzy, A. S.

In: Dental Materials, 12.06.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - An in vitro study of a novel quaternary ammonium silane endodontic irrigant

AU - Daood, U.

AU - Parolia, A.

AU - Elkezza, A.

AU - Yiu, C. K.

AU - Abbott, P.

AU - Matinlinna, J. P.

AU - Fawzy, A. S.

PY - 2019/6/12

Y1 - 2019/6/12

N2 - Objective: To analyze effect of NaOCl + 2% quaternary ammonium silane (QAS)-containing novel irrigant against bacteria impregnated inside the root canal system, and to evaluate its antimicrobial and mechanical potential of dentine substrate. Methods: Root canal was prepared using stainless steel K-files™ and ProTaper™ and subjected to manual and ultrasonic irrigation using 6% NaOCl + 2% CHX, 6% NaOCl + 2% QAS and saline as control. For confocal-microscopy, Raman spectroscopy and SEM analysis before and after treatment, Enterococcus faecalis cultured for 7 days. Raman spectroscopy analysis was done across cut section of gutta percha/sealer-dentine to detect resin infiltration. Indentation of mechanical properties was evaluated using a Berkovich indenter. The contact angle of irrigants and surface free energy were evaluated. Mineralization nodules were detected through Alazarin red after 14 days. Results: Control biofilms showed dense green colonies. Majority of E. faecalis bacteria were present in biofilm fluoresced red in NaOCl + 2% QAS group. There was reduction of 484 cm−1 Raman band and its intensity reached lowest with NaOCl + 2% QAS. There was an increase in 1350–1420 cm−1 intensity in the NaOCl + 2% CHX groups. Gradual decrease in 1639 cm−1 and 1609 cm−1 Raman signal ratios were seen in the resin-depth region of 17 μm>, 14.1 μm> and 13.2 μm for NaOCl + 2% QAS, NaOCl + 2% CHX and control groups respectively. All obturated groups showed an intact sealer/dentine interface with a few notable differences. 0.771 and 83.5% creep indentation distance for NaOCl + 2% QAS ultrasonic groups were observed. Highest proportion of polar component was significantly found in the NaOCl + 2% QAS groups which was significantly higher as compared to other groups. Mineralized nodules were increased in NaOCl + 2% QAS. Significance: Favorable antimicrobial and endodontic profile of the NaOCl + 2% QAS solution might suggest clinical use for it for more predictable reduction of intracanal bacteria.

AB - Objective: To analyze effect of NaOCl + 2% quaternary ammonium silane (QAS)-containing novel irrigant against bacteria impregnated inside the root canal system, and to evaluate its antimicrobial and mechanical potential of dentine substrate. Methods: Root canal was prepared using stainless steel K-files™ and ProTaper™ and subjected to manual and ultrasonic irrigation using 6% NaOCl + 2% CHX, 6% NaOCl + 2% QAS and saline as control. For confocal-microscopy, Raman spectroscopy and SEM analysis before and after treatment, Enterococcus faecalis cultured for 7 days. Raman spectroscopy analysis was done across cut section of gutta percha/sealer-dentine to detect resin infiltration. Indentation of mechanical properties was evaluated using a Berkovich indenter. The contact angle of irrigants and surface free energy were evaluated. Mineralization nodules were detected through Alazarin red after 14 days. Results: Control biofilms showed dense green colonies. Majority of E. faecalis bacteria were present in biofilm fluoresced red in NaOCl + 2% QAS group. There was reduction of 484 cm−1 Raman band and its intensity reached lowest with NaOCl + 2% QAS. There was an increase in 1350–1420 cm−1 intensity in the NaOCl + 2% CHX groups. Gradual decrease in 1639 cm−1 and 1609 cm−1 Raman signal ratios were seen in the resin-depth region of 17 μm>, 14.1 μm> and 13.2 μm for NaOCl + 2% QAS, NaOCl + 2% CHX and control groups respectively. All obturated groups showed an intact sealer/dentine interface with a few notable differences. 0.771 and 83.5% creep indentation distance for NaOCl + 2% QAS ultrasonic groups were observed. Highest proportion of polar component was significantly found in the NaOCl + 2% QAS groups which was significantly higher as compared to other groups. Mineralized nodules were increased in NaOCl + 2% QAS. Significance: Favorable antimicrobial and endodontic profile of the NaOCl + 2% QAS solution might suggest clinical use for it for more predictable reduction of intracanal bacteria.

KW - Antimicrobial

KW - Condensation

KW - Confocal microscopy

KW - E. faecalis

KW - Quaternary ammonium silane

KW - Raman spectroscopy

KW - Root dentine

UR - http://www.scopus.com/inward/record.url?scp=85067043716&partnerID=8YFLogxK

U2 - 10.1016/j.dental.2019.05.020

DO - 10.1016/j.dental.2019.05.020

M3 - Article

JO - Dental Materials

JF - Dental Materials

SN - 0109-5641

ER -