Abstract
Objective: To analyze effect of NaOCl + 2% quaternary ammonium silane (QAS)-containing novel irrigant against bacteria impregnated inside the root canal system, and to evaluate its antimicrobial and mechanical potential of dentine substrate. Methods: Root canal was prepared using stainless steel K-files™ and ProTaper™ and subjected to manual and ultrasonic irrigation using 6% NaOCl + 2% CHX, 6% NaOCl + 2% QAS and saline as control. For confocal-microscopy, Raman spectroscopy and SEM analysis before and after treatment, Enterococcus faecalis cultured for 7 days. Raman spectroscopy analysis was done across cut section of gutta percha/sealer-dentine to detect resin infiltration. Indentation of mechanical properties was evaluated using a Berkovich indenter. The contact angle of irrigants and surface free energy were evaluated. Mineralization nodules were detected through Alazarin red after 14 days. Results: Control biofilms showed dense green colonies. Majority of E. faecalis bacteria were present in biofilm fluoresced red in NaOCl + 2% QAS group. There was reduction of 484 cm−1 Raman band and its intensity reached lowest with NaOCl + 2% QAS. There was an increase in 1350–1420 cm−1 intensity in the NaOCl + 2% CHX groups. Gradual decrease in 1639 cm−1 and 1609 cm−1 Raman signal ratios were seen in the resin-depth region of 17 μm>, 14.1 μm> and 13.2 μm for NaOCl + 2% QAS, NaOCl + 2% CHX and control groups respectively. All obturated groups showed an intact sealer/dentine interface with a few notable differences. 0.771 and 83.5% creep indentation distance for NaOCl + 2% QAS ultrasonic groups were observed. Highest proportion of polar component was significantly found in the NaOCl + 2% QAS groups which was significantly higher as compared to other groups. Mineralized nodules were increased in NaOCl + 2% QAS. Significance: Favorable antimicrobial and endodontic profile of the NaOCl + 2% QAS solution might suggest clinical use for it for more predictable reduction of intracanal bacteria.
| Original language | English |
|---|---|
| Journal | Dental Materials |
| DOIs | |
| Publication status | E-pub ahead of print - 12 Jun 2019 |
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An in vitro study of a novel quaternary ammonium silane endodontic irrigant. / Daood, U.; Parolia, A.; Elkezza, A.; Yiu, C. K.; Abbott, P.; Matinlinna, J. P.; Fawzy, A. S.
In: Dental Materials, 12.06.2019.Research output: Contribution to journal › Article
TY - JOUR
T1 - An in vitro study of a novel quaternary ammonium silane endodontic irrigant
AU - Daood, U.
AU - Parolia, A.
AU - Elkezza, A.
AU - Yiu, C. K.
AU - Abbott, P.
AU - Matinlinna, J. P.
AU - Fawzy, A. S.
PY - 2019/6/12
Y1 - 2019/6/12
N2 - Objective: To analyze effect of NaOCl + 2% quaternary ammonium silane (QAS)-containing novel irrigant against bacteria impregnated inside the root canal system, and to evaluate its antimicrobial and mechanical potential of dentine substrate. Methods: Root canal was prepared using stainless steel K-files™ and ProTaper™ and subjected to manual and ultrasonic irrigation using 6% NaOCl + 2% CHX, 6% NaOCl + 2% QAS and saline as control. For confocal-microscopy, Raman spectroscopy and SEM analysis before and after treatment, Enterococcus faecalis cultured for 7 days. Raman spectroscopy analysis was done across cut section of gutta percha/sealer-dentine to detect resin infiltration. Indentation of mechanical properties was evaluated using a Berkovich indenter. The contact angle of irrigants and surface free energy were evaluated. Mineralization nodules were detected through Alazarin red after 14 days. Results: Control biofilms showed dense green colonies. Majority of E. faecalis bacteria were present in biofilm fluoresced red in NaOCl + 2% QAS group. There was reduction of 484 cm−1 Raman band and its intensity reached lowest with NaOCl + 2% QAS. There was an increase in 1350–1420 cm−1 intensity in the NaOCl + 2% CHX groups. Gradual decrease in 1639 cm−1 and 1609 cm−1 Raman signal ratios were seen in the resin-depth region of 17 μm>, 14.1 μm> and 13.2 μm for NaOCl + 2% QAS, NaOCl + 2% CHX and control groups respectively. All obturated groups showed an intact sealer/dentine interface with a few notable differences. 0.771 and 83.5% creep indentation distance for NaOCl + 2% QAS ultrasonic groups were observed. Highest proportion of polar component was significantly found in the NaOCl + 2% QAS groups which was significantly higher as compared to other groups. Mineralized nodules were increased in NaOCl + 2% QAS. Significance: Favorable antimicrobial and endodontic profile of the NaOCl + 2% QAS solution might suggest clinical use for it for more predictable reduction of intracanal bacteria.
AB - Objective: To analyze effect of NaOCl + 2% quaternary ammonium silane (QAS)-containing novel irrigant against bacteria impregnated inside the root canal system, and to evaluate its antimicrobial and mechanical potential of dentine substrate. Methods: Root canal was prepared using stainless steel K-files™ and ProTaper™ and subjected to manual and ultrasonic irrigation using 6% NaOCl + 2% CHX, 6% NaOCl + 2% QAS and saline as control. For confocal-microscopy, Raman spectroscopy and SEM analysis before and after treatment, Enterococcus faecalis cultured for 7 days. Raman spectroscopy analysis was done across cut section of gutta percha/sealer-dentine to detect resin infiltration. Indentation of mechanical properties was evaluated using a Berkovich indenter. The contact angle of irrigants and surface free energy were evaluated. Mineralization nodules were detected through Alazarin red after 14 days. Results: Control biofilms showed dense green colonies. Majority of E. faecalis bacteria were present in biofilm fluoresced red in NaOCl + 2% QAS group. There was reduction of 484 cm−1 Raman band and its intensity reached lowest with NaOCl + 2% QAS. There was an increase in 1350–1420 cm−1 intensity in the NaOCl + 2% CHX groups. Gradual decrease in 1639 cm−1 and 1609 cm−1 Raman signal ratios were seen in the resin-depth region of 17 μm>, 14.1 μm> and 13.2 μm for NaOCl + 2% QAS, NaOCl + 2% CHX and control groups respectively. All obturated groups showed an intact sealer/dentine interface with a few notable differences. 0.771 and 83.5% creep indentation distance for NaOCl + 2% QAS ultrasonic groups were observed. Highest proportion of polar component was significantly found in the NaOCl + 2% QAS groups which was significantly higher as compared to other groups. Mineralized nodules were increased in NaOCl + 2% QAS. Significance: Favorable antimicrobial and endodontic profile of the NaOCl + 2% QAS solution might suggest clinical use for it for more predictable reduction of intracanal bacteria.
KW - Antimicrobial
KW - Condensation
KW - Confocal microscopy
KW - E. faecalis
KW - Quaternary ammonium silane
KW - Raman spectroscopy
KW - Root dentine
UR - http://www.scopus.com/inward/record.url?scp=85067043716&partnerID=8YFLogxK
U2 - 10.1016/j.dental.2019.05.020
DO - 10.1016/j.dental.2019.05.020
M3 - Article
JO - Dental Materials
JF - Dental Materials
SN - 0109-5641
ER -