An in-depth investigation of orexin receptor pharmacology profiled using advanced Bioluminescence Resonance Energy Transfer (BRET) techniques

Natasha Dale

Research output: ThesisDoctoral Thesis

33 Downloads (Pure)

Abstract

The orexin system is a hypothalamic modulatory system that regulates arousal states and feeding behaviour. The system comprises two G protein coupled receptors (GPCRs), orexin receptor 1 (OxR1) and orexin receptor 2 (OxR2) and two neuropeptides, orexin A (OxA) and orexin B (OxB). This thesis utilises Bioluminescence Resonance Energy Transfer(BRET) to conduct an in-depth investigation of the pharmacology of the orexin receptors. The results of this thesis show pharmacologically-relevant spatiotemporal control of orexin receptor signalling. These findings warrant further investigation to determine the relevance of these trends to different cell types and small molecule orexin agonists.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • The University of Western Australia
Supervisors/Advisors
  • Pfleger, Kevin, Supervisor
  • Johnstone, Liz, Supervisor
Thesis sponsors
Award date19 Dec 2022
DOIs
Publication statusUnpublished - 2022

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