TY - JOUR
T1 - An improved reversed-phase liquid chromatographic method for the analysis of low-molecular mass organic acids in plant root exudates
AU - Cawthray, Greg
PY - 2003
Y1 - 2003
N2 - Reversed-phase column liquid chromatography (RPLC) was used for the separation and quantification of 10 low-molecular-mass organic acids (malic, malonic, lactic, acetic, maleic, citric, cis-aconitic, succinic, fumaric, and trans-aconitic) in plant root exudates. A mobile phase of 93% 25 mM KH2PO4 at pH 2.5 and 7% methanol at a flow-rate of I ml min(-1) resolved all 10 acids in 15 min on a C-18 column. Experiments demonstrated a significant (P
AB - Reversed-phase column liquid chromatography (RPLC) was used for the separation and quantification of 10 low-molecular-mass organic acids (malic, malonic, lactic, acetic, maleic, citric, cis-aconitic, succinic, fumaric, and trans-aconitic) in plant root exudates. A mobile phase of 93% 25 mM KH2PO4 at pH 2.5 and 7% methanol at a flow-rate of I ml min(-1) resolved all 10 acids in 15 min on a C-18 column. Experiments demonstrated a significant (P
U2 - 10.1016/S0021-9673(03)01129-4
DO - 10.1016/S0021-9673(03)01129-4
M3 - Article
VL - 1011
SP - 223
EP - 240
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
ER -