An Eya1-Notch axis specifies bipotential epibranchial differentiation in mammalian craniofacial morphogenesis

Haoran Zhang, Li Wang, Elaine Yee Man Wong, Sze Lan Tsang, Pin-Xian Xu, Urban Lendahl, Mai Har Sham

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Craniofacial morphogenesis requires proper development of pharyngeal arches and epibranchial placodes. We show that the epibranchial placodes, in addition to giving rise to cranial sensory neurons, generate a novel lineage-related non-neuronal cell population for mouse pharyngeal arch development. Eya1 is essential for the development of epibranchial placodes and proximal pharyngeal arches. We identify an Eya1-Notch regulatory axis that specifies both the neuronal and non-neuronal commitment of the epibranchial placode, where Notch acts downstream of Eya1 and promotes the non-neuronal cell fate. Notch is regulated by the threonine phosphatase activity of Eya1. Eya1 dephosphorylates p-threonine-2122 of the Notch1 intracellular domain (Notch1 ICD), which increases the stability of Notch1 ICD and maintains Notch signaling activity in the non-neuronal epibranchial placodal cells. Our data unveil a more complex differentiation program in epibranchial placodes and an important role for the Eya1-Notch axis in craniofacial morphogenesis.

Original languageEnglish
Article numbere30126
JournaleLife
Volume6
DOIs
Publication statusPublished - 15 Nov 2017
Externally publishedYes

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Branchial Region
Arches
Morphogenesis
Phosphoprotein Phosphatases
Sensory Receptor Cells
Threonine
Neurons
Cells
Population

Cite this

Zhang, Haoran ; Wang, Li ; Wong, Elaine Yee Man ; Tsang, Sze Lan ; Xu, Pin-Xian ; Lendahl, Urban ; Sham, Mai Har. / An Eya1-Notch axis specifies bipotential epibranchial differentiation in mammalian craniofacial morphogenesis. In: eLife. 2017 ; Vol. 6.
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An Eya1-Notch axis specifies bipotential epibranchial differentiation in mammalian craniofacial morphogenesis. / Zhang, Haoran; Wang, Li; Wong, Elaine Yee Man; Tsang, Sze Lan; Xu, Pin-Xian; Lendahl, Urban; Sham, Mai Har.

In: eLife, Vol. 6, e30126, 15.11.2017.

Research output: Contribution to journalArticle

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