An evaluation of methods for measurement of pesticides in sorption experiments

S. Baskaran, N. S. Bolan

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


The concentration of four pesticides (2,4-D, atrazine, phorate, and terbufos) in soil solution during sorption experiments was measured using UV spectrophotometry, Gas Liquid Chromatography (GLC), High Performance Liquid Chromatography (HPLC), and radiotracer technique. The presence of water soluble organic matter in soil solution interfered with the measurement of pesticide using the UV spectrophotometry. The use of GLC, HPLC, and radiotracer technique involving 14C gave a good estimate of the concentration of pesticide in soil solution. The pesticide remaining in soil can be quantitatively analyzed by extracting with a scintillation solution containing an organic solvent such as toluene or dioxane. Among the various centrifuge tubes glass tubes with Teflon caps sorbed negligible amount of pesticides and these tubes can be used for the sorption measurements.

Original languageEnglish
Pages (from-to)369-380
Number of pages12
JournalCommunications in Soil Science and Plant Analysis
Issue number3-4
Publication statusPublished - 1998
Externally publishedYes


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