An evaluation of freezing as a preservation technique for analyzing dissolved organic C, N and P in surface water samples

Jason Fellman, D.V. D'Amore, E. Hood

    Research output: Contribution to journalArticle

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    Abstract

    Techniques for preserving surface water samples are recently in demand because of the increased interest in quantifying dissolved organic matter (DOM) in surface waters and the frequent collection of samples in remote locations. Freezing is a common technique employed by many researchers for preserving surface water samples; however, there has been little evaluation of the effects of freezing on DOM concentrations. Ten streams were sampled in southeast Alaska with a range of dissolved organic carbon (DOC) concentrations (1.5 to 39 mg C L− 1) to evaluate the influence of freezing (flash and standard freeze) and filter pore size (0.2 and 0.7 μm nominal pore size) on dissolved organic C, N and P concentrations. We report a significant decrease in DOC (p <0.005) and total dissolved P (p <0.005) concentrations when streamwater samples were frozen, whereas concentrations of dissolved organic N did not significantly decrease after freezing (p = 0.06). We further show that when surface water samples were frozen, there was a decrease in the specific ultraviolet absorbance (SUVA) of DOC that is particularly evident with high concentrations of DOC. This finding suggests that spectroscopic properties of DOC have the potential to be used as indicators of whether surface water samples can be frozen. Our results lead us to recommend that surface water samples with high DOC concentrations (> 5 mg C L− 1) and/or samples with high SUVA values (> 3.5–4 L mg-C− 1 m− 1) should be analyzed immediately and not frozen. © 2007 Elsevier B.V. All rights reserved.
    Original languageEnglish
    Pages (from-to)305-312
    JournalScience of the Total Environment
    Volume392
    Issue number2-3
    DOIs
    Publication statusPublished - 2008

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