@article{df0c73a5a0d64ff6970c58024b5876e0,
title = "An environmental DNA approach to informing restoration of the functionally extinct oyster, Ostrea angasi",
abstract = "The success of oyster reef restoration can be enhanced by data on the distribution of remnant populations to inform the selection of suitable restoration locations. A quantitative polymerase chain reaction-based environmental DNA (eDNA) assay was designed to provide distribution data for the oyster, Ostrea angasi, whose reefs are functionally extinct in Port Phillip Bay, Australia. Ostrea angasi eDNA accumulation and decay was measured in aquaria containing oysters in low and high densities, prior to testing the efficacy of the eDNA approach for detection of oysters at 15 field sites. Ostrea angasi eDNA accumulated significantly faster in aquaria where more individuals were present, while eDNA became undetectable 2–6 days after oysters were removed in low-density treatments. The eDNA samples were successful at detecting O. angasi in the field when taken in close proximity of an oyster population. Increasing the sample number and volume could maximize oyster detection, demonstrating the potential of eDNA to identify suitable sites for the restoration of functionally extinct marine ecosystems.",
keywords = "CO1, detectability, ecosystem engineer, environmental DNA, native species, oyster reef restoration",
author = "Alexander Coutts and Allyson O'Brien and Weeks, \{Andrew R.\} and Swearer, \{Stephen E.\} and \{van Rooyen\}, Anthony and Simon Branigan and Morris, \{Rebecca L\}",
note = "Funding Information: We thank L. Adams, T. Graham, R. Sharma, D. Chamberlain, S. Suebsanguan, B. Cleveland, L. Barrett, S. Elliot‐Kerr, E. Barbier and R. Zhu for their help with field work. We also thank S. Song, S. Licul and A. Ghodke for their help in the laboratory and Wayne O'Connor for providing the sample used in probe validation. We additionally thank Kade Mills, Nicole Mertens and the Victorian National Parks Association's Reef Watch Programme who provided advice on site sampling through the OysterWatch Programme. This work was funded through the Port Phillip Bay Fund (awarded to The Nature Conservancy for the OysterWatch Project, sub‐contracted to R.L.M. and S.E.S.) by the Victorian Government's Department of Environment, Land, Water and Planning. The National Centre for Coasts and Climate is funded through the Earth Systems and Climate Change Hub by the Australian Government's National Environmental Science Program. Open access publishing facilitated by The University of Melbourne, as part of the Wiley ‐ The University of Melbourne agreement via the Council of Australian University Librarians. Open access publishing facilitated by The University of Melbourne, as part of the Wiley ‐ The University of Melbourne agreement via the Council of Australian University Librarians. O. stentina Funding Information: We thank L. Adams, T. Graham, R. Sharma, D. Chamberlain, S. Suebsanguan, B. Cleveland, L. Barrett, S. Elliot-Kerr, E. Barbier and R. Zhu for their help with field work. We also thank S. Song, S. Licul and A. Ghodke for their help in the laboratory and Wayne O'Connor for providing the O. stentina sample used in probe validation. We additionally thank Kade Mills, Nicole Mertens and the Victorian National Parks Association's Reef Watch Programme who provided advice on site sampling through the OysterWatch Programme. This work was funded through the Port Phillip Bay Fund (awarded to The Nature Conservancy for the OysterWatch Project, sub-contracted to R.L.M. and S.E.S.) by the Victorian Government's Department of Environment, Land, Water and Planning. The National Centre for Coasts and Climate is funded through the Earth Systems and Climate Change Hub by the Australian Government's National Environmental Science Program. Open access publishing facilitated by The University of Melbourne, as part of the Wiley - The University of Melbourne agreement via the Council of Australian University Librarians. Open access publishing facilitated by The University of Melbourne, as part of the Wiley - The University of Melbourne agreement via the Council of Australian University Librarians. Funding Information: This work was funded through the Port Phillip Bay Fund (awarded to The Nature Conservancy) by the Victorian Government's Department of Environment, Land, Water and Planning. The Nature Conservancy contributed to selection of field sites based on the results of the OysterWatch Project. Funding information Publisher Copyright: {\textcopyright} 2022 The Authors. Aquatic Conservation: Marine and Freshwater Ecosystems published by John Wiley \& Sons Ltd.",
year = "2022",
month = nov,
doi = "10.1002/aqc.3886",
language = "English",
volume = "32",
pages = "1732--1744",
journal = "Aquatic Conservation: Marine and Freshwater Ecosystems",
issn = "1052-7613",
publisher = "Royal College of Psychiatrists",
number = "11",
}
SDG 6 Clean Water and Sanitation
SDG 14 Life Below Water
SDG 15 Life on Land