An emulsion technique for quantitating high affinity uptake of postprandial lipoproteins in vivo : foundations for a diagnostic assay

Hanni Gennat

    Research output: ThesisDoctoral Thesis

    64 Downloads (Pure)

    Abstract

    [Truncated] The metabolism of chylomicrons and their remnants is delayed in certain disease states. Several studies have shown that the LDL-receptor is the primary mechanism for the removal of chylomicron remnants from plasma; down regulation of this receptor may therefore result in a delay in the hepatic clearance of these particles. However, at present there is no simple method by which high-affinity (receptor) uptake mechanisms can be detected in humans. Therefore, the principal aim of this thesis was to develop an emulsion technique for quantitating high affinity uptake of chylomicron remnants in vivo. The assay is based on the clearance patterns of two chylomicron-like lipid emulsions. The clearance of normal emulsion represents total uptake from plasma via high and low affinity mechanisms; in contrast, modified emulsions do not interact with receptor mechanisms and are cleared via low-affinity mechanisms only. The difference between the clearances of these two emulsions gives a measure of high-affinity uptake of postprandial lipoproteins.
    Original languageEnglish
    QualificationDoctor of Philosophy
    Awarding Institution
    • The University of Western Australia
    DOIs
    Publication statusUnpublished - 2002

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