An acute dietary intervention in lactation has variable impacts on the maternal and infant microbiota and milk antimicrobial proteins

Azhar Sindi

doi:10.26182/gs82-3c13

An acute dietary intervention in lactation has variable impacts on the maternal and infant microbiota and milk antimicrobial proteins

Azhar Sindi

UWA Medical School

Research output: Thesis › Doctoral Thesis

152 Downloads (Pure)

Abstract

This thesis showed that maternal factors, such as body mass index (BMI), delivery mode, breast pump use, and infant pacifier use were associated to human milk (HM) bacterial profiles in an Australian cohort. It also demonstrated that maternal dietary intervention during lactation resulted in minor changes in maternal microbiota and significant shifts in the infant gut microbiome functional potential, but not its composition. The intervention also reduced HM lactoferrin and lysozyme levels, with daily and circadian fluctuations. These findings suggest maternal and infant factors, alongside diet, can influence the HM microbiome and antimicrobial protein, impacting infant gut microbiome

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Geddes, Donna, Supervisor Payne, Matt, Supervisor Stinson, Lisa, Supervisor
Award date	23 Jul 2024
DOIs	https://doi.org/10.26182/gs82-3c13
Publication status	Unpublished - 2024

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10.26182/gs82-3c13

THESIS - DOCTOR OF PHILOSOPHY - SINDI Azhar Salem M - 2024
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1 Active

Prediction of preterm birth using vaginal microbiology
Newnham, J. (Investigator 01), Doherty, D. (Investigator 02), White, S. (Investigator 03), Brown, K. (Investigator 04), Peel, M. (Investigator 05) & Payne, M. (Investigator 06)
NHMRC National Health and Medical Research Council
1/01/22 → 31/12/26
Project: Research

3 Article
1 Review article

Maternal dietary intervention during lactation impacts the maternal faecal and human milk microbiota
Sindi, A. S., Stinson, L. F., Gridneva, Z., Leghi, G. E., Netting, M. J., Wlodek, M. E., Muhlhausler, B. S., Rea, A., Trevenen, M. L., Geddes, D. T. & Payne, M. S., May 2024, In: Journal of Applied Microbiology. 135, 5, 15 p., lxae024.
Research output: Contribution to journal › Article › peer-review

Open Access
2 Citations (Scopus)
Characterisation of human milk bacterial DNA profiles in a small cohort of Australian women in relation to infant and maternal factors
Sindi, A., Cheema, A. S., Trevenen, M., Geddes, D., Payne, M. & Stinson, L., 25 Jan 2023, In: PLoS One. 18, 1 January, 15 p., e0280960.
Research output: Contribution to journal › Article › peer-review

Open Access
6 Citations (Scopus)
Effect of a reduced fat and sugar maternal dietary intervention during lactation on the infant gut microbiome
Sindi, A. S., Stinson, L. F., Lean, S. S., Chooi, Y. H., Leghi, G. E., Netting, M. J., Wlodek, M. E., Muhlhausler, B. S., Geddes, D. T. & Payne, M. S., 17 Aug 2022, In: Frontiers in Microbiology. 13, 21 p., 900702.
Research output: Contribution to journal › Article › peer-review

Open Access
12 Citations (Scopus)

Cite this

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title = "An acute dietary intervention in lactation has variable impacts on the maternal and infant microbiota and milk antimicrobial proteins",

abstract = "This thesis showed that maternal factors, such as body mass index (BMI), delivery mode, breast pump use, and infant pacifier use were associated to human milk (HM) bacterial profiles in an Australian cohort. It also demonstrated that maternal dietary intervention during lactation resulted in minor changes in maternal microbiota and significant shifts in the infant gut microbiome functional potential, but not its composition. The intervention also reduced HM lactoferrin and lysozyme levels, with daily and circadian fluctuations. These findings suggest maternal and infant factors, alongside diet, can influence the HM microbiome and antimicrobial protein, impacting infant gut microbiome",

keywords = "Human milk microbiome, maternal gut microbiome, infant gut microbiome, maternal diet, lactation, shotgun metagenomic, 16s rRNA gene sequencing",

author = "Azhar Sindi",

year = "2024",

doi = "10.26182/gs82-3c13",

language = "English",

school = "The University of Western Australia",

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AU - Sindi, Azhar

PY - 2024

Y1 - 2024

N2 - This thesis showed that maternal factors, such as body mass index (BMI), delivery mode, breast pump use, and infant pacifier use were associated to human milk (HM) bacterial profiles in an Australian cohort. It also demonstrated that maternal dietary intervention during lactation resulted in minor changes in maternal microbiota and significant shifts in the infant gut microbiome functional potential, but not its composition. The intervention also reduced HM lactoferrin and lysozyme levels, with daily and circadian fluctuations. These findings suggest maternal and infant factors, alongside diet, can influence the HM microbiome and antimicrobial protein, impacting infant gut microbiome

AB - This thesis showed that maternal factors, such as body mass index (BMI), delivery mode, breast pump use, and infant pacifier use were associated to human milk (HM) bacterial profiles in an Australian cohort. It also demonstrated that maternal dietary intervention during lactation resulted in minor changes in maternal microbiota and significant shifts in the infant gut microbiome functional potential, but not its composition. The intervention also reduced HM lactoferrin and lysozyme levels, with daily and circadian fluctuations. These findings suggest maternal and infant factors, alongside diet, can influence the HM microbiome and antimicrobial protein, impacting infant gut microbiome

KW - Human milk microbiome

KW - maternal gut microbiome

KW - infant gut microbiome

KW - maternal diet

KW - lactation

KW - shotgun metagenomic

KW - 16s rRNA gene sequencing

U2 - 10.26182/gs82-3c13

DO - 10.26182/gs82-3c13

M3 - Doctoral Thesis

ER -

An acute dietary intervention in lactation has variable impacts on the maternal and infant microbiota and milk antimicrobial proteins

Abstract

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Projects

Prediction of preterm birth using vaginal microbiology

Research output

Maternal dietary intervention during lactation impacts the maternal faecal and human milk microbiota

Characterisation of human milk bacterial DNA profiles in a small cohort of Australian women in relation to infant and maternal factors

Effect of a reduced fat and sugar maternal dietary intervention during lactation on the infant gut microbiome

Cite this