TY - JOUR
T1 - Ammonia stress on a resilient mesophilic anaerobic inoculum
T2 - Methane production, microbial community, and putative metabolic pathways
AU - Buhlmann, C.H.
AU - Mickan, Bede
AU - Jenkins, Sasha
AU - Tait, Stephan
AU - Kahandawala, T.K.A
AU - Bahri, P.A.
PY - 2019/3
Y1 - 2019/3
N2 - Short term inhibition tests, 16S rRNA tag sequencing and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt), were employed to visualise the effects of increasing total ammoniacal nitrogen (TAN) concentration (3400–10166 ppm TAN) on microbial community structure and metabolic pathways for acetate degradation. The rate of methane production on acetate was significantly reduced by TAN concentrations above 6133 ppm; however, methane continued to be produced, even at 10166 ppm TAN (0.026 ± 0.0003 gCOD.gVS−1 inoculum.day−1). Hydrogenotrophic methanogenesis with syntrophic acetate oxidation (SAO) was identified as the dominant pathway for methane production. A shift towards SAO pathways at higher TAN concentrations and a decrease in the number of ‘gene hits’ for key genes in specific methanogenesis pathways was observed. Overall, the results highlighted potential for inhibition activity testing to be used together with PICRUSt, to estimate changes in microbial metabolism and to better understand microbial resilience in industrial AD facilities. © 2018 Elsevier Ltd
AB - Short term inhibition tests, 16S rRNA tag sequencing and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt), were employed to visualise the effects of increasing total ammoniacal nitrogen (TAN) concentration (3400–10166 ppm TAN) on microbial community structure and metabolic pathways for acetate degradation. The rate of methane production on acetate was significantly reduced by TAN concentrations above 6133 ppm; however, methane continued to be produced, even at 10166 ppm TAN (0.026 ± 0.0003 gCOD.gVS−1 inoculum.day−1). Hydrogenotrophic methanogenesis with syntrophic acetate oxidation (SAO) was identified as the dominant pathway for methane production. A shift towards SAO pathways at higher TAN concentrations and a decrease in the number of ‘gene hits’ for key genes in specific methanogenesis pathways was observed. Overall, the results highlighted potential for inhibition activity testing to be used together with PICRUSt, to estimate changes in microbial metabolism and to better understand microbial resilience in industrial AD facilities. © 2018 Elsevier Ltd
U2 - 10.1016/j.biortech.2018.12.012
DO - 10.1016/j.biortech.2018.12.012
M3 - Article
C2 - 30579103
SN - 0960-8524
VL - 275
SP - 70
EP - 77
JO - Bioresource Technology
JF - Bioresource Technology
ER -