Despite the significant consequences of fibrotic diseases there remains no widely effective therapeutic approach. This thesis aimed to investigate changes in the genome and proteome that maybe targeted to treat fibrosis. A role for DNA G-quadruplexes and Lysyl oxidase (LOX) enzyme activity in regulating extracellular matrix deposition was investigated. In addition, RNASeq and metabolic tracing were used to better understand the dynamic changes in gene and protein expression linked to fibrosis. The study provided significant new insight in o the biology of fibrosis and evidence to support novel therapeutic approaches, for example the potential to target quadruplex formation, for treating fibrosis.