ALS-FTLD associated mutations of SQSTM1 impact on Keap1-Nrf2 signalling

A. Goode, Sarah Rea, Melanie Sultana, B. Shaw, M.S. Searle, R. Layfield

Research output: Contribution to journalArticle

23 Citations (Scopus)
505 Downloads (Pure)

Abstract

© 2016 The AuthorsThe transcription factor Nrf2 and its repressor protein Keap1 play key roles in the regulation of antioxidant stress responses and both Keap1-Nrf2 signalling and oxidative stress have been implicated in the pathogenesis of the ALS-FTLD spectrum of neurodegenerative disorders. The Keap1-binding partner and autophagy receptor SQSTM1/p62 has also recently been linked genetically to ALS-FTLD, with some missense mutations identified in patients mapping within or close to its Keap1-interacting region (KIR, residues 347–352). Here we report the effects on protein function of four different disease associated mutations of SQSTM1/p62 which affect the KIR region. Only mutations mapping precisely to the KIR (P348L and G351A) were associated with a loss of Keap1 binding in co-immunoprecipitations comparable to wild-type SQSTM1/p62. These selective effects on Keap1 recognition were entirely rational based on protein structural models. Consistent with impaired Keap1 binding, the P348L and G351A KIR mutants showed reduced ability to activate Nrf2 signalling compared to wild-type SQSTM1/p62 in antioxidant response element (ARE)-luciferase reporter assays. The results suggest that SQSTM1 mutations within the KIR of SQSTM1/p62 contribute to aetiology of some cases of ALS-FTLD through a mechanism involving aberrant expression or regulation of oxidative response genes.
Original languageEnglish
Pages (from-to)52-58
Number of pages7
JournalMolecular and Cellular Neuroscience
Volume76
Early online date20 Aug 2016
DOIs
Publication statusPublished - Oct 2016

Fingerprint Dive into the research topics of 'ALS-FTLD associated mutations of <em>SQSTM1</em> impact on Keap1-Nrf2 signalling'. Together they form a unique fingerprint.

  • Projects

    Cite this