Allergen-enhanced thrombomodulin (blood dendritic cell antigen 3, CD141) expression on dendritic cells is associated with a TH2-skewed immune response

S.T. Yerkovich, M. Roponen, Merran Smith, Katherine Mckenna, Anthony Bosco, L.S. Subrata, E. Mamessier, Matthew Wikstrom, P. Le Souef, Peter Sly, Patrick Holt, John Upham

Research output: Contribution to journalArticlepeer-review

60 Citations (Scopus)

Abstract

Background: Dendritic cells (DCs) are important in allergic diseases such as asthma, although little is known regarding the mechanisms by which DCs induce TH2-polarized responses in atopic individuals. It has been suggested that intrinsic properties of allergens can directly stimulate TH2 polarizing functions of DCs, but little is known of the underlying mechanisms. Objective: To identify novel genes expressed by house dust mite (HDM) allergen–exposed DCs. Methods: We screened for allergen-induced gene expression by microarray, and validated differentially expressed genes at the mRNA and protein levels.Results: Thrombomodulin (CD141, blood dendritic cell antigen 3) expression by microarray was higher on HDM-stimulated DCs from atopic (relative to nonatopic) individuals. These findings were confirmed at both the mRNA and protein levels in an independent group. Purified thrombomodulin+ DCs induced a strongly TH2-polarized cytokine response by allergen-specific T cells compared with DCs lacking thrombomodulin. In vivo, thrombomodulin+ circulating DCs were significantly more frequent in subjects with HDM allergy and asthma, compared with control subjects. Furthermore, thrombomodulin expression in blood leukocytes was higher in children with acute asthma than at convalescence 6 weeks later.Conclusion: Thrombomodulin expression on DCs may be involved in the pathogenesis of atopy and asthma.
Original languageEnglish
Pages (from-to)209-216.e4
JournalJournal of Allergy and Clinical Immunology
Volume123
Issue number1
DOIs
Publication statusPublished - 2009

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