TY - JOUR
T1 - Airway mechanics and methods used to visualize smooth muscle dynamics in vitro
AU - Cooper, P.R.
AU - Mcparland, B.E.
AU - Mitchell, Howard
AU - Noble, Peter
AU - Politi, A.Z.
AU - Ressmeyer, A.R.
AU - West, Adrian
PY - 2009
Y1 - 2009
N2 - Contraction of airway smooth muscle (ASM) is regulated by the physiological, structural and mechanical environment in the lung. We review two in vitro techniques, lung slices and airway segment preparations, that enable in situ ASM contraction and airway narrowing to be visualized. Lung slices and airway segment approaches bridge a gap between cell culture and isolated ASM, and whole animal studies. Imaging techniques enable key upstream events involved in airway narrowing, such as ASM cell signalling and structural and mechanical events impinging on ASM, to be investigated.
AB - Contraction of airway smooth muscle (ASM) is regulated by the physiological, structural and mechanical environment in the lung. We review two in vitro techniques, lung slices and airway segment preparations, that enable in situ ASM contraction and airway narrowing to be visualized. Lung slices and airway segment approaches bridge a gap between cell culture and isolated ASM, and whole animal studies. Imaging techniques enable key upstream events involved in airway narrowing, such as ASM cell signalling and structural and mechanical events impinging on ASM, to be investigated.
U2 - 10.1016/j.pupt.2008.09.008
DO - 10.1016/j.pupt.2008.09.008
M3 - Article
C2 - 19041411
VL - 22
SP - 398
EP - 406
JO - Pulmonary Pharmacology & Therapeutics
JF - Pulmonary Pharmacology & Therapeutics
SN - 1094-5539
IS - 5
ER -