Advances in BAC-based physical mapping and map integration strategies in plants

Ruvini Ariyadasa, Nils Stein

Research output: Contribution to journalReview articlepeer-review

28 Citations (Scopus)

Abstract

In the advent of next-generation sequencing (NGS) platforms, map-based sequencing strategy has been recently suppressed being too expensive and laborious. The detailed studies on NGS drafts alone indicated these assemblies remain far from gold standard reference quality, especially when applied on complex genomes. In this context the conventional BAC-based physical mapping has been identified as an important intermediate layer in current hybrid sequencing strategy. BAC-based physical map construction and its integration with high-density genetic maps have benefited from NGS and high-throughput array platforms. This paper addresses the current advancements of BAC-based physical mapping and high-throughput map integration strategies to obtain densely anchored well-ordered physical maps. The resulted maps are of immediate utility while providing a template to harness the maximum benefits of the current NGS platforms.

Original languageEnglish
Article number184854
JournalJournal of Biomedicine and Biotechnology
Volume2012
DOIs
Publication statusPublished - 2012
Externally publishedYes

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