Adult granulosa cell tumours (GCT): Clinicopathological outcomes including FOXL2 mutational status and expression

R. Rosario, M. Wilson, W. Cheng, K. Payne, Paul Cohen, P. Fong, A.N.S. Shelling

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    Abstract

    Objectives The aim of this research was to use nucleic acids isolated from formalin-fixed paraffin-embedded (FFPE) tissue to investigate the diagnostic potential and prognostic significance of FOXL2 in adult-type GCTs, particularly as a marker of identifying early stage patients that are likely to relapse. Methods We performed a retrospective review of GCT patients referred to the Auckland Gynae-Oncology Multidisciplinary Team from 1955 to 2012. Baseline characteristics, clinical course, histopathology and survival data was recorded. Using nucleic acids extracted from FFPE tumour blocks, FOXL2 mutation status and expression was determined by DNA sequencing and RT-qPCR, respectively, and correlated with clinical data. Results 57 adult GCT patients were identified, however FFPE tumour blocks were available for only 37 of these patients. Sequencing results confirmed the presence of the FOXL2 mutation in 70% of patients. FOXL2 mutation positive adult tumours showed a trend towards higher FOXL2 expression than wildtype adult tumours, particularly in stage I patients (p = 0.051). In addition, patients with homozygous FOXL2 mutations had a significantly higher relapse rate (p = 0.04). There was no significant correlation between FOXL2 mutation status or FOXL2 expression and any other clinical variables. Conclusions FFPE tumour blocks are a valuable resource of molecular information, especially when studying rare tumours such as GCTs. The FOXL2 mutation appears to have some diagnostic potential, however additional work in a larger cohort needs to be completed to confirm the prognostic significance of this gene mutation, and its expression. © 2013 Elsevier Inc.
    Original languageEnglish
    Pages (from-to)325-329
    JournalGynecologic Oncology
    Volume131
    Issue number2
    DOIs
    Publication statusPublished - 2013

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    Granulosa Cell Tumor
    Mutation
    Paraffin
    Formaldehyde
    Neoplasms
    Nucleic Acids
    Recurrence
    DNA Sequence Analysis
    Survival
    Research

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    Rosario, R. ; Wilson, M. ; Cheng, W. ; Payne, K. ; Cohen, Paul ; Fong, P. ; Shelling, A.N.S. / Adult granulosa cell tumours (GCT): Clinicopathological outcomes including FOXL2 mutational status and expression. In: Gynecologic Oncology. 2013 ; Vol. 131, No. 2. pp. 325-329.
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    abstract = "Objectives The aim of this research was to use nucleic acids isolated from formalin-fixed paraffin-embedded (FFPE) tissue to investigate the diagnostic potential and prognostic significance of FOXL2 in adult-type GCTs, particularly as a marker of identifying early stage patients that are likely to relapse. Methods We performed a retrospective review of GCT patients referred to the Auckland Gynae-Oncology Multidisciplinary Team from 1955 to 2012. Baseline characteristics, clinical course, histopathology and survival data was recorded. Using nucleic acids extracted from FFPE tumour blocks, FOXL2 mutation status and expression was determined by DNA sequencing and RT-qPCR, respectively, and correlated with clinical data. Results 57 adult GCT patients were identified, however FFPE tumour blocks were available for only 37 of these patients. Sequencing results confirmed the presence of the FOXL2 mutation in 70{\%} of patients. FOXL2 mutation positive adult tumours showed a trend towards higher FOXL2 expression than wildtype adult tumours, particularly in stage I patients (p = 0.051). In addition, patients with homozygous FOXL2 mutations had a significantly higher relapse rate (p = 0.04). There was no significant correlation between FOXL2 mutation status or FOXL2 expression and any other clinical variables. Conclusions FFPE tumour blocks are a valuable resource of molecular information, especially when studying rare tumours such as GCTs. The FOXL2 mutation appears to have some diagnostic potential, however additional work in a larger cohort needs to be completed to confirm the prognostic significance of this gene mutation, and its expression. {\circledC} 2013 Elsevier Inc.",
    author = "R. Rosario and M. Wilson and W. Cheng and K. Payne and Paul Cohen and P. Fong and A.N.S. Shelling",
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    Adult granulosa cell tumours (GCT): Clinicopathological outcomes including FOXL2 mutational status and expression. / Rosario, R.; Wilson, M.; Cheng, W.; Payne, K.; Cohen, Paul; Fong, P.; Shelling, A.N.S.

    In: Gynecologic Oncology, Vol. 131, No. 2, 2013, p. 325-329.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Adult granulosa cell tumours (GCT): Clinicopathological outcomes including FOXL2 mutational status and expression

    AU - Rosario, R.

    AU - Wilson, M.

    AU - Cheng, W.

    AU - Payne, K.

    AU - Cohen, Paul

    AU - Fong, P.

    AU - Shelling, A.N.S.

    PY - 2013

    Y1 - 2013

    N2 - Objectives The aim of this research was to use nucleic acids isolated from formalin-fixed paraffin-embedded (FFPE) tissue to investigate the diagnostic potential and prognostic significance of FOXL2 in adult-type GCTs, particularly as a marker of identifying early stage patients that are likely to relapse. Methods We performed a retrospective review of GCT patients referred to the Auckland Gynae-Oncology Multidisciplinary Team from 1955 to 2012. Baseline characteristics, clinical course, histopathology and survival data was recorded. Using nucleic acids extracted from FFPE tumour blocks, FOXL2 mutation status and expression was determined by DNA sequencing and RT-qPCR, respectively, and correlated with clinical data. Results 57 adult GCT patients were identified, however FFPE tumour blocks were available for only 37 of these patients. Sequencing results confirmed the presence of the FOXL2 mutation in 70% of patients. FOXL2 mutation positive adult tumours showed a trend towards higher FOXL2 expression than wildtype adult tumours, particularly in stage I patients (p = 0.051). In addition, patients with homozygous FOXL2 mutations had a significantly higher relapse rate (p = 0.04). There was no significant correlation between FOXL2 mutation status or FOXL2 expression and any other clinical variables. Conclusions FFPE tumour blocks are a valuable resource of molecular information, especially when studying rare tumours such as GCTs. The FOXL2 mutation appears to have some diagnostic potential, however additional work in a larger cohort needs to be completed to confirm the prognostic significance of this gene mutation, and its expression. © 2013 Elsevier Inc.

    AB - Objectives The aim of this research was to use nucleic acids isolated from formalin-fixed paraffin-embedded (FFPE) tissue to investigate the diagnostic potential and prognostic significance of FOXL2 in adult-type GCTs, particularly as a marker of identifying early stage patients that are likely to relapse. Methods We performed a retrospective review of GCT patients referred to the Auckland Gynae-Oncology Multidisciplinary Team from 1955 to 2012. Baseline characteristics, clinical course, histopathology and survival data was recorded. Using nucleic acids extracted from FFPE tumour blocks, FOXL2 mutation status and expression was determined by DNA sequencing and RT-qPCR, respectively, and correlated with clinical data. Results 57 adult GCT patients were identified, however FFPE tumour blocks were available for only 37 of these patients. Sequencing results confirmed the presence of the FOXL2 mutation in 70% of patients. FOXL2 mutation positive adult tumours showed a trend towards higher FOXL2 expression than wildtype adult tumours, particularly in stage I patients (p = 0.051). In addition, patients with homozygous FOXL2 mutations had a significantly higher relapse rate (p = 0.04). There was no significant correlation between FOXL2 mutation status or FOXL2 expression and any other clinical variables. Conclusions FFPE tumour blocks are a valuable resource of molecular information, especially when studying rare tumours such as GCTs. The FOXL2 mutation appears to have some diagnostic potential, however additional work in a larger cohort needs to be completed to confirm the prognostic significance of this gene mutation, and its expression. © 2013 Elsevier Inc.

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    JF - Gynecologic Oncology

    SN - 0090-8258

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