Adipogenic differentiation of adipose tissue derived adult stem cells in nude mouse

Yu Suk Choi, Sang Myun Cha, Young Yeol Lee, Seok Woon Kwon, Chan Jeoung Park, MiJung Kim

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

We evaluated the use of a combination of adipose tissue derived adult stem cells (ADSCs) obtained from liposuction and injectable poly(lactic-co-glycolic acid) (PLGA) spheres for adipose tissue engineering. Adipogenesis was examined in nude mice injected subcutaneously with ADSCs (group I), PLGA spheres (group II), or ADSCs attached PLGA spheres (group III) cultured in adipogenic medium for 7 days. After 4 and 8 weeks, newly formed adipose tissue was observed in groups II and III but not in group I. Oil red O staining of newly formed tissue showed that there was substantially more tissue regeneration and adipogenic differentiation in group III than in group II. RT-PCR confirmed that, after 8 weeks, the PLGA-attached ADSCs had fully differentiated into adipocytes. This study provides significant evidence that ADSCs and PLGA spheres can be used in a clinical setting to generate adipose tissue as a noninvasive soft tissue filler.

Original languageEnglish
Pages (from-to)631-7
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume345
Issue number2
DOIs
Publication statusPublished - 30 Jun 2006
Externally publishedYes

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