TY - JOUR
T1 - Additively manufactured polyethylene terephthalate scaffolds for scapholunate interosseous ligament reconstruction
AU - Gomez-Cerezo, M. Natividad
AU - Perevoshchikova, Nataliya
AU - Ruan, Rui
AU - Moerman, Kevin M.
AU - Bindra, Randy
AU - Lloyd, David G.
AU - Zheng, Ming Hao
AU - Saxby, David J.
AU - Vaquette, Cedryck
N1 - Funding Information:
The authors acknowledge the facilities and the scientific and technical assistance of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy and Microanalysis, The University of Queensland. The authors also acknowledge the Translational Research Institute (TRI) for providing the excellent research environment and core facilities (Kamil Sokolowski, Preclinical Imaging) that enabled this research. The authors also acknowledge the Lions Club of Australia and the Mater Foundation for funding the Skyscan 1272 Micro-CT. This research was funded by an Australian MTPConnect Biomedical Technology Horizons grant to RB, DGL, CV, DJS and MHZ, and grants in support from Griffith University .
Funding Information:
Cedryck Vaquette reports financial support was provided by MTPConnect Biomedical Technology Horizons. Cedryck Vaquette reports a relationship with MTPConnect Biomedical Technology Horizons that includes: funding grants. Cedryck Vaquette, Randy Bindra have a patent #PCT/AU2018/000133 issued to U.S. Patent and Trademark Office.The authors acknowledge the facilities and the scientific and technical assistance of the Australian Microscopy & Microanalysis Research Facility at the Centre for Microscopy and Microanalysis, The University of Queensland. The authors also acknowledge the Translational Research Institute (TRI) for providing the excellent research environment and core facilities (Kamil Sokolowski, Preclinical Imaging) that enabled this research. The authors also acknowledge the Lions Club of Australia and the Mater Foundation for funding the Skyscan 1272 Micro-CT. This research was funded by an Australian MTPConnect Biomedical Technology Horizons grant to RB, DGL, CV, DJS and MHZ, and grants in support from Griffith University.
Publisher Copyright:
© 2023 Elsevier B.V.
PY - 2023/6
Y1 - 2023/6
N2 - The regeneration of the ruptured scapholunate interosseous ligament (SLIL) represents a clinical challenge. Here, we propose the use of a Bone-Ligament-Bone (BLB) 3D-printed polyethylene terephthalate (PET) scaffold for achieving mechanical stabilisation of the scaphoid and lunate following SLIL rupture. The BLB scaffold featured two bone compartments bridged by aligned fibres (ligament compartment) mimicking the architecture of the native tissue. The scaffold presented tensile stiffness in the range of 260 ± 38 N/mm and ultimate load of 113 ± 13 N, which would support physiological loading. A finite element analysis (FEA), using inverse finite element analysis (iFEA) for material property identification, showed an adequate fit between simulation and experimental data. The scaffold was then biofunctionalized using two different methods: injected with a Gelatin Methacryloyl solution containing human mesenchymal stem cell spheroids (hMSC) or seeded with tendon-derived stem cells (TDSC) and placed in a bioreactor to undergo cyclic deformation. The first approach demonstrated high cell viability, as cells migrated out of the spheroid and colonised the interstitial space of the scaffold. These cells adopted an elongated morphology suggesting the internal architecture of the scaffold exerted topographical guidance. The second method demonstrated the high resilience of the scaffold to cyclic deformation and the secretion of a fibroblastic related protein was enhanced by the mechanical stimulation. This process promoted the expression of relevant proteins, such as Tenomodulin (TNMD), indicating mechanical stimulation may enhance cell differentiation and be useful prior to surgical implantation. In conclusion, the PET scaffold presented several promising characteristics for the immediate mechanical stabilisation of disassociated scaphoid and lunate and, in the longer-term, the regeneration of the ruptured SLIL.
AB - The regeneration of the ruptured scapholunate interosseous ligament (SLIL) represents a clinical challenge. Here, we propose the use of a Bone-Ligament-Bone (BLB) 3D-printed polyethylene terephthalate (PET) scaffold for achieving mechanical stabilisation of the scaphoid and lunate following SLIL rupture. The BLB scaffold featured two bone compartments bridged by aligned fibres (ligament compartment) mimicking the architecture of the native tissue. The scaffold presented tensile stiffness in the range of 260 ± 38 N/mm and ultimate load of 113 ± 13 N, which would support physiological loading. A finite element analysis (FEA), using inverse finite element analysis (iFEA) for material property identification, showed an adequate fit between simulation and experimental data. The scaffold was then biofunctionalized using two different methods: injected with a Gelatin Methacryloyl solution containing human mesenchymal stem cell spheroids (hMSC) or seeded with tendon-derived stem cells (TDSC) and placed in a bioreactor to undergo cyclic deformation. The first approach demonstrated high cell viability, as cells migrated out of the spheroid and colonised the interstitial space of the scaffold. These cells adopted an elongated morphology suggesting the internal architecture of the scaffold exerted topographical guidance. The second method demonstrated the high resilience of the scaffold to cyclic deformation and the secretion of a fibroblastic related protein was enhanced by the mechanical stimulation. This process promoted the expression of relevant proteins, such as Tenomodulin (TNMD), indicating mechanical stimulation may enhance cell differentiation and be useful prior to surgical implantation. In conclusion, the PET scaffold presented several promising characteristics for the immediate mechanical stabilisation of disassociated scaphoid and lunate and, in the longer-term, the regeneration of the ruptured SLIL.
KW - 3D-printing
KW - Hand surgery
KW - Polyethylene terephthalate (PET)
KW - Scapholunate interosseous ligament
KW - Tissue-engineering
UR - http://www.scopus.com/inward/record.url?scp=85151562061&partnerID=8YFLogxK
U2 - 10.1016/j.bioadv.2023.213397
DO - 10.1016/j.bioadv.2023.213397
M3 - Article
C2 - 37023566
AN - SCOPUS:85151562061
SN - 2772-9508
VL - 149
JO - Biomaterials Advances
JF - Biomaterials Advances
M1 - 213397
ER -