Additive and epistatic interactions between AKR and AIN loci conferring bluegreen aphid resistance and hypersensitivity in Medicago truncatula

Lars G. Kamphuis, John P. Klingler, Silke Jacques, Ling Ling Gao, Owain R. Edwards, Karam B. Singh

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Aphids, including the bluegreen aphid (BGA; Acyrthosiphon kondoi), are important pests in agriculture. Two BGA resistance genes have been identified in the model legume Medicago truncatula, namely AKR (Acyrthosiphon kondoi resistance) and AIN (Acyrthosiphon induced necrosis). In this study, progeny derived from a cross between a resistant accession named Jester and a highly susceptible accession named A20 were used to study the interaction between the AKR and AIN loci with respect to BGA performance and plant response to BGA infestation. These studies demonstrated that AKR and AIN have additive effects on the BGA resistance phenotype. However, AKR exerts dominant suppression epistasis on AIN-controlled macroscopic necrotic lesions. Nevertheless, both AKR and AIN condition production of H2O2 at the BGA feeding site. Electrical penetration graph analysis demonstrated that AKR prevents phloem sap ingestion, irrespective of the presence of AIN. Similarly, the jasmonic acid defense signaling pathway is recruited by AKR, irrespective of AIN. This research identifies an enhancement of aphid resistance through gene stacking, and insights into the interaction of distinct resistance genes against insect pests.

Original languageEnglish
Pages (from-to)4887-4901
Number of pages15
JournalJournal of Experimental Botany
Volume70
Issue number18
DOIs
Publication statusPublished - 15 Sep 2019

Fingerprint

Acyrthosiphon
Acyrthosiphon kondoi
Medicago truncatula
Aphids
hypersensitive response
Aphidoidea
Hypersensitivity
necrosis
Necrosis
loci
Insect Genes
Phloem
genes
Agriculture
epistasis
Fabaceae
Genes
jasmonic acid
additive effect
lesions (animal)

Cite this

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title = "Additive and epistatic interactions between AKR and AIN loci conferring bluegreen aphid resistance and hypersensitivity in Medicago truncatula",
abstract = "Aphids, including the bluegreen aphid (BGA; Acyrthosiphon kondoi), are important pests in agriculture. Two BGA resistance genes have been identified in the model legume Medicago truncatula, namely AKR (Acyrthosiphon kondoi resistance) and AIN (Acyrthosiphon induced necrosis). In this study, progeny derived from a cross between a resistant accession named Jester and a highly susceptible accession named A20 were used to study the interaction between the AKR and AIN loci with respect to BGA performance and plant response to BGA infestation. These studies demonstrated that AKR and AIN have additive effects on the BGA resistance phenotype. However, AKR exerts dominant suppression epistasis on AIN-controlled macroscopic necrotic lesions. Nevertheless, both AKR and AIN condition production of H2O2 at the BGA feeding site. Electrical penetration graph analysis demonstrated that AKR prevents phloem sap ingestion, irrespective of the presence of AIN. Similarly, the jasmonic acid defense signaling pathway is recruited by AKR, irrespective of AIN. This research identifies an enhancement of aphid resistance through gene stacking, and insights into the interaction of distinct resistance genes against insect pests.",
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Additive and epistatic interactions between AKR and AIN loci conferring bluegreen aphid resistance and hypersensitivity in Medicago truncatula. / Kamphuis, Lars G.; Klingler, John P.; Jacques, Silke; Gao, Ling Ling; Edwards, Owain R.; Singh, Karam B.

In: Journal of Experimental Botany, Vol. 70, No. 18, 15.09.2019, p. 4887-4901.

Research output: Contribution to journalArticle

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T1 - Additive and epistatic interactions between AKR and AIN loci conferring bluegreen aphid resistance and hypersensitivity in Medicago truncatula

AU - Kamphuis, Lars G.

AU - Klingler, John P.

AU - Jacques, Silke

AU - Gao, Ling Ling

AU - Edwards, Owain R.

AU - Singh, Karam B.

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Y1 - 2019/9/15

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