Acute hyperglycaemia does not alter nitric oxide-mediated microvascular function in the skin of adolescents with type 1 diabetes

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Abstract

Purpose
We assessed the impact of an acute bout of hyperglycaemia on nitric oxide (NO)-mediated microvascular function in the skin of adolescents with type 1 diabetes (T1DM).

Methods
Twelve subjects (12–18 years) with T1DM were randomised into a control (n = 6) or hyperglycaemia (n = 6) group. Hyperinsulinaemic clamps were used to manipulate blood glucose level (BGL). Following a baseline period, where all subjects were euglycaemic (20 min), the experimental phase began. During the experimental phase, BGL was elevated to 16.7 ± 0.9 mmol L−1 in the hyperglyceamic group, while it was maintained at euglycaemia (5.5 ± 0.1 mmol L−1) in the control group. Simultaneously, cutaneous microvascular function (% max cutaneous vascular conductance, CVC%) was assessed using laser Doppler fluxometry following stimulation of skin blood flow using localised heating (42 °C). To determine the NO contribution to skin blood flow, two microdialysis sites were assessed, one perfused with Ringers and the other with the NO blocker, NG-monomethyl-l-arginine (l-NMMA).

Results
In the hyperglycaemic group, acute increase in BGL was not associated with changes in skin blood flow (CVC% 82.4 ± 8.7 % at 5.5 ± 0.1 mmol L−1 vs 79.5 ± 9.1 % at 16.7 ± 0.9 mmol L−1, unpaired t tests, P = 0.588) or the contribution of NO to vasodilation.

Conclusions
These results suggest that, in our group of adolescents with type 1 diabetes, acute hyperglycaemia did not affect skin microvascular NO-mediated function.
Original languageEnglish
Pages (from-to)435-441
Number of pages7
JournalEuropean Journal of Applied Physiology
Volume114
Issue number2
DOIs
Publication statusPublished - Feb 2014

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