Abstract
| Original language | English |
|---|---|
| Title of host publication | Plant Mitochondria: Methods and Protocols |
| Editors | J. Whelan, M.W. Murcha |
| Place of Publication | United States of America |
| Publisher | Springer |
| Pages | 139-149 |
| Volume | 1305 |
| ISBN (Print) | 9781493926398 |
| DOIs | |
| Publication status | Published - 2015 |
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Activity assay for plant mitochondrial enzymes. / Huang, Shaobai; Lee, Alex; Harvey Millar, Harvey.
Plant Mitochondria: Methods and Protocols. ed. / J. Whelan; M.W. Murcha. Vol. 1305 United States of America : Springer, 2015. p. 139-149.Research output: Chapter in Book/Conference paper › Chapter
TY - CHAP
T1 - Activity assay for plant mitochondrial enzymes
AU - Huang, Shaobai
AU - Lee, Alex
AU - Harvey Millar, Harvey
PY - 2015
Y1 - 2015
N2 - © 2015, Springer Science+Business Media New York. Mitochondria are sites for respiration to produce chemical energy via oxidative phosphorylation. Their primary role has been viewed as the oxidation of organic acids via the tricarboxylic acid (TCA) cycle and the synthesis of ATP coupled to the transfer of electrons to O2. TCA cycle enzymes are essential for plant carbon metabolism and provide the reductant for the electron transport chain (ETC) enzymes that in turn drives ATP synthesis. The activity of individual enzymes will determine the flux of metabolism and thus the downstream consequences for respiration rate. Measurements of activities of mitochondrial enzymes, such as components of TCA cycle and the ETC, can provide insight into regulation of mitochondrial function. The activities of these enzymes vary between developmental stages, in different tissues, and in response to environmental conditions. In this chapter, methods for enzymatic assay of TCA cycle enzymes and a number of the ETC complex enzymes are described in detail.
AB - © 2015, Springer Science+Business Media New York. Mitochondria are sites for respiration to produce chemical energy via oxidative phosphorylation. Their primary role has been viewed as the oxidation of organic acids via the tricarboxylic acid (TCA) cycle and the synthesis of ATP coupled to the transfer of electrons to O2. TCA cycle enzymes are essential for plant carbon metabolism and provide the reductant for the electron transport chain (ETC) enzymes that in turn drives ATP synthesis. The activity of individual enzymes will determine the flux of metabolism and thus the downstream consequences for respiration rate. Measurements of activities of mitochondrial enzymes, such as components of TCA cycle and the ETC, can provide insight into regulation of mitochondrial function. The activities of these enzymes vary between developmental stages, in different tissues, and in response to environmental conditions. In this chapter, methods for enzymatic assay of TCA cycle enzymes and a number of the ETC complex enzymes are described in detail.
U2 - 10.1007/978-1-4939-2639-8_10
DO - 10.1007/978-1-4939-2639-8_10
M3 - Chapter
SN - 9781493926398
VL - 1305
SP - 139
EP - 149
BT - Plant Mitochondria: Methods and Protocols
A2 - Whelan, J.
A2 - Murcha, M.W.
PB - Springer
CY - United States of America
ER -