Activity assay for plant mitochondrial enzymes

Research output: Chapter in Book/Conference paperChapter

5 Citations (Scopus)

Abstract

© 2015, Springer Science+Business Media New York. Mitochondria are sites for respiration to produce chemical energy via oxidative phosphorylation. Their primary role has been viewed as the oxidation of organic acids via the tricarboxylic acid (TCA) cycle and the synthesis of ATP coupled to the transfer of electrons to O2. TCA cycle enzymes are essential for plant carbon metabolism and provide the reductant for the electron transport chain (ETC) enzymes that in turn drives ATP synthesis. The activity of individual enzymes will determine the flux of metabolism and thus the downstream consequences for respiration rate. Measurements of activities of mitochondrial enzymes, such as components of TCA cycle and the ETC, can provide insight into regulation of mitochondrial function. The activities of these enzymes vary between developmental stages, in different tissues, and in response to environmental conditions. In this chapter, methods for enzymatic assay of TCA cycle enzymes and a number of the ETC complex enzymes are described in detail.
Original languageEnglish
Title of host publicationPlant Mitochondria: Methods and Protocols
EditorsJ. Whelan, M.W. Murcha
Place of PublicationUnited States of America
PublisherSpringer
Pages139-149
Volume1305
ISBN (Print)9781493926398
DOIs
Publication statusPublished - 2015

Fingerprint

Citric Acid Cycle
Enzymes
Electron Transport
Adenosine Triphosphate
Oxidative Phosphorylation
Reducing Agents
Enzyme Assays
Respiratory Rate
Mitochondria
Respiration
Carbon
Electrons
Acids

Cite this

Huang, S., Lee, A., & Harvey Millar, H. (2015). Activity assay for plant mitochondrial enzymes. In J. Whelan, & M. W. Murcha (Eds.), Plant Mitochondria: Methods and Protocols (Vol. 1305, pp. 139-149). United States of America: Springer. https://doi.org/10.1007/978-1-4939-2639-8_10
Huang, Shaobai ; Lee, Alex ; Harvey Millar, Harvey. / Activity assay for plant mitochondrial enzymes. Plant Mitochondria: Methods and Protocols. editor / J. Whelan ; M.W. Murcha. Vol. 1305 United States of America : Springer, 2015. pp. 139-149
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Huang, S, Lee, A & Harvey Millar, H 2015, Activity assay for plant mitochondrial enzymes. in J Whelan & MW Murcha (eds), Plant Mitochondria: Methods and Protocols. vol. 1305, Springer, United States of America, pp. 139-149. https://doi.org/10.1007/978-1-4939-2639-8_10

Activity assay for plant mitochondrial enzymes. / Huang, Shaobai; Lee, Alex; Harvey Millar, Harvey.

Plant Mitochondria: Methods and Protocols. ed. / J. Whelan; M.W. Murcha. Vol. 1305 United States of America : Springer, 2015. p. 139-149.

Research output: Chapter in Book/Conference paperChapter

TY - CHAP

T1 - Activity assay for plant mitochondrial enzymes

AU - Huang, Shaobai

AU - Lee, Alex

AU - Harvey Millar, Harvey

PY - 2015

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N2 - © 2015, Springer Science+Business Media New York. Mitochondria are sites for respiration to produce chemical energy via oxidative phosphorylation. Their primary role has been viewed as the oxidation of organic acids via the tricarboxylic acid (TCA) cycle and the synthesis of ATP coupled to the transfer of electrons to O2. TCA cycle enzymes are essential for plant carbon metabolism and provide the reductant for the electron transport chain (ETC) enzymes that in turn drives ATP synthesis. The activity of individual enzymes will determine the flux of metabolism and thus the downstream consequences for respiration rate. Measurements of activities of mitochondrial enzymes, such as components of TCA cycle and the ETC, can provide insight into regulation of mitochondrial function. The activities of these enzymes vary between developmental stages, in different tissues, and in response to environmental conditions. In this chapter, methods for enzymatic assay of TCA cycle enzymes and a number of the ETC complex enzymes are described in detail.

AB - © 2015, Springer Science+Business Media New York. Mitochondria are sites for respiration to produce chemical energy via oxidative phosphorylation. Their primary role has been viewed as the oxidation of organic acids via the tricarboxylic acid (TCA) cycle and the synthesis of ATP coupled to the transfer of electrons to O2. TCA cycle enzymes are essential for plant carbon metabolism and provide the reductant for the electron transport chain (ETC) enzymes that in turn drives ATP synthesis. The activity of individual enzymes will determine the flux of metabolism and thus the downstream consequences for respiration rate. Measurements of activities of mitochondrial enzymes, such as components of TCA cycle and the ETC, can provide insight into regulation of mitochondrial function. The activities of these enzymes vary between developmental stages, in different tissues, and in response to environmental conditions. In this chapter, methods for enzymatic assay of TCA cycle enzymes and a number of the ETC complex enzymes are described in detail.

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DO - 10.1007/978-1-4939-2639-8_10

M3 - Chapter

SN - 9781493926398

VL - 1305

SP - 139

EP - 149

BT - Plant Mitochondria: Methods and Protocols

A2 - Whelan, J.

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PB - Springer

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Huang S, Lee A, Harvey Millar H. Activity assay for plant mitochondrial enzymes. In Whelan J, Murcha MW, editors, Plant Mitochondria: Methods and Protocols. Vol. 1305. United States of America: Springer. 2015. p. 139-149 https://doi.org/10.1007/978-1-4939-2639-8_10