Activity assay for plant mitochondrial enzymes

Research output: Chapter in Book/Conference paperChapter

10 Citations (Scopus)

Abstract

© 2015, Springer Science+Business Media New York. Mitochondria are sites for respiration to produce chemical energy via oxidative phosphorylation. Their primary role has been viewed as the oxidation of organic acids via the tricarboxylic acid (TCA) cycle and the synthesis of ATP coupled to the transfer of electrons to O2. TCA cycle enzymes are essential for plant carbon metabolism and provide the reductant for the electron transport chain (ETC) enzymes that in turn drives ATP synthesis. The activity of individual enzymes will determine the flux of metabolism and thus the downstream consequences for respiration rate. Measurements of activities of mitochondrial enzymes, such as components of TCA cycle and the ETC, can provide insight into regulation of mitochondrial function. The activities of these enzymes vary between developmental stages, in different tissues, and in response to environmental conditions. In this chapter, methods for enzymatic assay of TCA cycle enzymes and a number of the ETC complex enzymes are described in detail.
Original languageEnglish
Title of host publicationPlant Mitochondria: Methods and Protocols
EditorsJ. Whelan, M.W. Murcha
Place of PublicationUnited States of America
PublisherSpringer
Pages139-149
Volume1305
ISBN (Print)9781493926398
DOIs
Publication statusPublished - 2015

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