Activation of human monocytes by granulocyte-macrophage colony-stimulating factor: Increased urokinase-type plasminogen activator activity

P. H. Hart, G. F. Vitti, D. R. Burgess, G. A. Whitty, K. Royston, J. A. Hamilton

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

Granulocyte-macrophage colony-stimulating factor (GM-CSF) raised the plasminogen activator (PA) activity of cultured human monocytes. This activity was characterized to be urokinase-PA (u-PA) by incubation with specific IgG and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis zymography. Increased u-PA activity reflected GM-CSF-induction of u-PA mRNA levels. The stimulatory properties of GM-CSF for monocyte PA activity differed from those of interleukin-4, which induced monocyte tissue-type PA (t-PA) activity, and of interferon-γ (IFN-γ), which alone was not stimulatory but augmented lipopolysaccharide-induced t-PA activity. GM-CSF alone did not stimulate detectable monocyte t-PA activity but combined with IFN-γ to promote this activity. Plasmin formation arising from GM-CSF-induced u-PA in monocytes may contribute to the matrix turnover involved in, eg, cell migration and inflammation, and may explain some of the pathology seen in GM-CSF transgenic mice.

Original languageEnglish
Pages (from-to)841-848
Number of pages8
JournalBlood
Volume77
Issue number4
Publication statusPublished - 1 Jan 1991
Externally publishedYes

Fingerprint

Dive into the research topics of 'Activation of human monocytes by granulocyte-macrophage colony-stimulating factor: Increased urokinase-type plasminogen activator activity'. Together they form a unique fingerprint.

Cite this