Activation of human monocytes by granulocyte-macrophage colony-stimulating factor: Increased urokinase-type plasminogen activator activity

P. H. Hart, G. F. Vitti, D. R. Burgess, G. A. Whitty, K. Royston, J. A. Hamilton

Research output: Contribution to journalArticle

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Abstract

Granulocyte-macrophage colony-stimulating factor (GM-CSF) raised the plasminogen activator (PA) activity of cultured human monocytes. This activity was characterized to be urokinase-PA (u-PA) by incubation with specific IgG and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis zymography. Increased u-PA activity reflected GM-CSF-induction of u-PA mRNA levels. The stimulatory properties of GM-CSF for monocyte PA activity differed from those of interleukin-4, which induced monocyte tissue-type PA (t-PA) activity, and of interferon-γ (IFN-γ), which alone was not stimulatory but augmented lipopolysaccharide-induced t-PA activity. GM-CSF alone did not stimulate detectable monocyte t-PA activity but combined with IFN-γ to promote this activity. Plasmin formation arising from GM-CSF-induced u-PA in monocytes may contribute to the matrix turnover involved in, eg, cell migration and inflammation, and may explain some of the pathology seen in GM-CSF transgenic mice.

Original languageEnglish
Pages (from-to)841-848
Number of pages8
JournalBlood
Volume77
Issue number4
Publication statusPublished - 1 Jan 1991
Externally publishedYes

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Urokinase-Type Plasminogen Activator
Granulocyte-Macrophage Colony-Stimulating Factor
Monocytes
Chemical activation
Plasminogen Activators
Interferons
Fibrinolysin
Tissue Plasminogen Activator
Pathology
Electrophoresis
Human Activities
Sodium Dodecyl Sulfate
Interleukin-4
Transgenic Mice
Cell Movement
Lipopolysaccharides
Polyacrylamide Gel Electrophoresis
Immunoglobulin G
Inflammation
Messenger RNA

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Hart, P. H., Vitti, G. F., Burgess, D. R., Whitty, G. A., Royston, K., & Hamilton, J. A. (1991). Activation of human monocytes by granulocyte-macrophage colony-stimulating factor: Increased urokinase-type plasminogen activator activity. Blood, 77(4), 841-848.
Hart, P. H. ; Vitti, G. F. ; Burgess, D. R. ; Whitty, G. A. ; Royston, K. ; Hamilton, J. A. / Activation of human monocytes by granulocyte-macrophage colony-stimulating factor : Increased urokinase-type plasminogen activator activity. In: Blood. 1991 ; Vol. 77, No. 4. pp. 841-848.
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abstract = "Granulocyte-macrophage colony-stimulating factor (GM-CSF) raised the plasminogen activator (PA) activity of cultured human monocytes. This activity was characterized to be urokinase-PA (u-PA) by incubation with specific IgG and by sodium dodecyl sulfate-polyacrylamide gel electrophoresis zymography. Increased u-PA activity reflected GM-CSF-induction of u-PA mRNA levels. The stimulatory properties of GM-CSF for monocyte PA activity differed from those of interleukin-4, which induced monocyte tissue-type PA (t-PA) activity, and of interferon-γ (IFN-γ), which alone was not stimulatory but augmented lipopolysaccharide-induced t-PA activity. GM-CSF alone did not stimulate detectable monocyte t-PA activity but combined with IFN-γ to promote this activity. Plasmin formation arising from GM-CSF-induced u-PA in monocytes may contribute to the matrix turnover involved in, eg, cell migration and inflammation, and may explain some of the pathology seen in GM-CSF transgenic mice.",
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Hart, PH, Vitti, GF, Burgess, DR, Whitty, GA, Royston, K & Hamilton, JA 1991, 'Activation of human monocytes by granulocyte-macrophage colony-stimulating factor: Increased urokinase-type plasminogen activator activity' Blood, vol. 77, no. 4, pp. 841-848.

Activation of human monocytes by granulocyte-macrophage colony-stimulating factor : Increased urokinase-type plasminogen activator activity. / Hart, P. H.; Vitti, G. F.; Burgess, D. R.; Whitty, G. A.; Royston, K.; Hamilton, J. A.

In: Blood, Vol. 77, No. 4, 01.01.1991, p. 841-848.

Research output: Contribution to journalArticle

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