Achyranthes bidentata polysaccharide suppresses osteoclastogenesis and bone resorption via inhibiting RANKL signaling

Dezhi Song, Zhen Cao, Song Huang, Jennifer Tickner, Nan Li, Heng Qiu, Xi Chen, Chao Wang, Kai Chen, Youqiang Sun, Shiwu Dong, Jiake Xu

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Abstract

Osteoclasts are highly differentiated multinucleated giant cells that play fundamental roles in bone resorption and in the pathogenesis of osteolytic conditions, such as osteoporosis and cancer-induced bone loss. Achyranthes bidentata polysaccharide (ABP) is a hydrophilic compound with anti-oxidation and anti-aging characteristics. The impact of ABP on RANKL-induced osteoclast formation and bone resorption has not been assessed, hence, in this study we investigated the effect of ABP on osteoclast formation and resorption in murine bone marrow derived osteoclasts. We found that ABP was able to suppress RANKL-induced osteoclast differentiation and bone resorption activity at concentrations above 6.5 µM, while demonstrating no cytotoxicity at concentrations up to 10 µM. The actions of ABP were mediated through inhibition of RANKL-induced c-Fos and NFATc1 gene and protein expression. Furthermore, we found that ABP suppressed NFATc1 transcriptional activity, and the phosphorylation of MAPK pathways induced by RANKL. Collectively, ABP attenuates RANKL-mediated osteoclast activity and signaling, and might serve as a potential therapeutic candidate for preventing bone loss related diseases.

Original languageEnglish
Pages (from-to)4826-4835
Number of pages10
JournalJournal of Cellular Biochemistry
Volume119
Issue number6
DOIs
Publication statusPublished - 1 Jun 2018

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Achyranthes
Bone Resorption
Osteogenesis
Osteoclasts
Polysaccharides
Bone
Proto-Oncogene Proteins c-fos
fos Genes
Bone Neoplasms
Phosphorylation
Giant Cells
Cytotoxicity
Osteoporosis
Aging of materials
Bone Marrow
Gene Expression
Bone and Bones
Oxidation

Cite this

Song, Dezhi ; Cao, Zhen ; Huang, Song ; Tickner, Jennifer ; Li, Nan ; Qiu, Heng ; Chen, Xi ; Wang, Chao ; Chen, Kai ; Sun, Youqiang ; Dong, Shiwu ; Xu, Jiake. / Achyranthes bidentata polysaccharide suppresses osteoclastogenesis and bone resorption via inhibiting RANKL signaling. In: Journal of Cellular Biochemistry. 2018 ; Vol. 119, No. 6. pp. 4826-4835.
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Achyranthes bidentata polysaccharide suppresses osteoclastogenesis and bone resorption via inhibiting RANKL signaling. / Song, Dezhi; Cao, Zhen; Huang, Song; Tickner, Jennifer; Li, Nan; Qiu, Heng; Chen, Xi; Wang, Chao; Chen, Kai; Sun, Youqiang; Dong, Shiwu; Xu, Jiake.

In: Journal of Cellular Biochemistry, Vol. 119, No. 6, 01.06.2018, p. 4826-4835.

Research output: Contribution to journalArticle

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T1 - Achyranthes bidentata polysaccharide suppresses osteoclastogenesis and bone resorption via inhibiting RANKL signaling

AU - Song, Dezhi

AU - Cao, Zhen

AU - Huang, Song

AU - Tickner, Jennifer

AU - Li, Nan

AU - Qiu, Heng

AU - Chen, Xi

AU - Wang, Chao

AU - Chen, Kai

AU - Sun, Youqiang

AU - Dong, Shiwu

AU - Xu, Jiake

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N2 - Osteoclasts are highly differentiated multinucleated giant cells that play fundamental roles in bone resorption and in the pathogenesis of osteolytic conditions, such as osteoporosis and cancer-induced bone loss. Achyranthes bidentata polysaccharide (ABP) is a hydrophilic compound with anti-oxidation and anti-aging characteristics. The impact of ABP on RANKL-induced osteoclast formation and bone resorption has not been assessed, hence, in this study we investigated the effect of ABP on osteoclast formation and resorption in murine bone marrow derived osteoclasts. We found that ABP was able to suppress RANKL-induced osteoclast differentiation and bone resorption activity at concentrations above 6.5 µM, while demonstrating no cytotoxicity at concentrations up to 10 µM. The actions of ABP were mediated through inhibition of RANKL-induced c-Fos and NFATc1 gene and protein expression. Furthermore, we found that ABP suppressed NFATc1 transcriptional activity, and the phosphorylation of MAPK pathways induced by RANKL. Collectively, ABP attenuates RANKL-mediated osteoclast activity and signaling, and might serve as a potential therapeutic candidate for preventing bone loss related diseases.

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