Acetyl-CoA: lyso-platelet-activating factor acetyltransferase activity in neutrophils from asthmatic and normal subjects

N.L.A. Misso, R.L. Gillon, M.L. Taylor, G.A. Stewart, Philip Thompson

Research output: Contribution to journalArticle

Abstract

1. Platelet-activating factor is a putative mediator of inflammation in asthma and the enzyme acetyl-CoA : lyso-platelet-activating factor acetyltransferase appears to be important in regulating platelet-activating factor production by leucocytes. To determine whether there are differences in acetyltransferase activity between asthmatic patients and normal subjects, enzyme activity was assayed in neutrophil lysates from atopic asthmatic patients (n=20), aspirin-sensitive asthmatic patients (n=12) and healthy, non-atopic, non-asthmatic control subjects (n=20), both basally and after stimulation with the calcium ionophore A23187.2. For a range of acetyl-CoA concentrations, acetyl-transferase activity (nmol of [acetyl-H-3]PAF min-1 mg-1 of protein) in unstimulated neutrophils from atopic asthmatic patients was significantly higher than that for normal subjects (P=0.038) and the mean V(max) for atopic asthmatic patients [18.4 (SD 6.9)nmol min-1 mg-1 of protein] was significantly greater than that for the control subjects [14.9(SD 4.6)nmol min-1 mg-1 of protein P
Original languageEnglish
Pages (from-to)455-463
JournalClinical Science
Volume85
Issue number10
Publication statusPublished - 1993

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1-alkylglycerophosphocholine acetyltransferase
Neutrophils
Platelet Activating Factor
Inflammation Mediators
Acetyl Coenzyme A
Proteins
Acetyltransferases
Calcium Ionophores
Enzymes
Transferases
Aspirin
Leukocytes
Asthma

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Misso, N. L. A., Gillon, R. L., Taylor, M. L., Stewart, G. A., & Thompson, P. (1993). Acetyl-CoA: lyso-platelet-activating factor acetyltransferase activity in neutrophils from asthmatic and normal subjects. Clinical Science, 85(10), 455-463.
Misso, N.L.A. ; Gillon, R.L. ; Taylor, M.L. ; Stewart, G.A. ; Thompson, Philip. / Acetyl-CoA: lyso-platelet-activating factor acetyltransferase activity in neutrophils from asthmatic and normal subjects. In: Clinical Science. 1993 ; Vol. 85, No. 10. pp. 455-463.
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Misso, NLA, Gillon, RL, Taylor, ML, Stewart, GA & Thompson, P 1993, 'Acetyl-CoA: lyso-platelet-activating factor acetyltransferase activity in neutrophils from asthmatic and normal subjects' Clinical Science, vol. 85, no. 10, pp. 455-463.

Acetyl-CoA: lyso-platelet-activating factor acetyltransferase activity in neutrophils from asthmatic and normal subjects. / Misso, N.L.A.; Gillon, R.L.; Taylor, M.L.; Stewart, G.A.; Thompson, Philip.

In: Clinical Science, Vol. 85, No. 10, 1993, p. 455-463.

Research output: Contribution to journalArticle

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T1 - Acetyl-CoA: lyso-platelet-activating factor acetyltransferase activity in neutrophils from asthmatic and normal subjects

AU - Misso, N.L.A.

AU - Gillon, R.L.

AU - Taylor, M.L.

AU - Stewart, G.A.

AU - Thompson, Philip

PY - 1993

Y1 - 1993

N2 - 1. Platelet-activating factor is a putative mediator of inflammation in asthma and the enzyme acetyl-CoA : lyso-platelet-activating factor acetyltransferase appears to be important in regulating platelet-activating factor production by leucocytes. To determine whether there are differences in acetyltransferase activity between asthmatic patients and normal subjects, enzyme activity was assayed in neutrophil lysates from atopic asthmatic patients (n=20), aspirin-sensitive asthmatic patients (n=12) and healthy, non-atopic, non-asthmatic control subjects (n=20), both basally and after stimulation with the calcium ionophore A23187.2. For a range of acetyl-CoA concentrations, acetyl-transferase activity (nmol of [acetyl-H-3]PAF min-1 mg-1 of protein) in unstimulated neutrophils from atopic asthmatic patients was significantly higher than that for normal subjects (P=0.038) and the mean V(max) for atopic asthmatic patients [18.4 (SD 6.9)nmol min-1 mg-1 of protein] was significantly greater than that for the control subjects [14.9(SD 4.6)nmol min-1 mg-1 of protein P

AB - 1. Platelet-activating factor is a putative mediator of inflammation in asthma and the enzyme acetyl-CoA : lyso-platelet-activating factor acetyltransferase appears to be important in regulating platelet-activating factor production by leucocytes. To determine whether there are differences in acetyltransferase activity between asthmatic patients and normal subjects, enzyme activity was assayed in neutrophil lysates from atopic asthmatic patients (n=20), aspirin-sensitive asthmatic patients (n=12) and healthy, non-atopic, non-asthmatic control subjects (n=20), both basally and after stimulation with the calcium ionophore A23187.2. For a range of acetyl-CoA concentrations, acetyl-transferase activity (nmol of [acetyl-H-3]PAF min-1 mg-1 of protein) in unstimulated neutrophils from atopic asthmatic patients was significantly higher than that for normal subjects (P=0.038) and the mean V(max) for atopic asthmatic patients [18.4 (SD 6.9)nmol min-1 mg-1 of protein] was significantly greater than that for the control subjects [14.9(SD 4.6)nmol min-1 mg-1 of protein P

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