TY - JOUR
T1 - Acellular Collagen Scaffold With Basic Fibroblast Growth Factor for Repair of Traumatic Tympanic Membrane Perforation in a Rat Model
AU - Yao, Xu
AU - Teh, Bing Mei
AU - Li, Huan
AU - Hu, Yi
AU - Huang, Juntao
AU - Lv, Cuiting
AU - Bu, Shizhong
AU - Zheng, Minghao
AU - Shen, Yi
PY - 2021/2
Y1 - 2021/2
N2 - Objective: To evaluate the efficacy of acellular collagen scaffold (ACS) in combination with basic fibroblast growth factor (bFGF) for the repair of traumatic tympanic membrane (TM) perforation in a rat model. Study Design: A prospective controlled animal study in a rat model of traumatic TM perforation. Setting: Tertiary medical center. Subjects and Methods: Sprague-Dawley rats (N = 84) with unilateral traumatic perforation of the right TMs were randomized to receive ACS, bFGF, ACS in combination with bFGF (ACS/bFGF), or nothing (spontaneous healing without any interventions as a control group). The healing outcomes were evaluated by otoscopy, optical coherence tomography, histology, and transmission electron microscopy at 1, 2, and 4 weeks postoperatively. The hearing outcomes were assessed with auditory brainstem response testing. Results: ACS/bFGF resulted in higher perforation closure rates at an earlier stage than spontaneous healing, ACS, and bFGF. Based on histology, optical coherence tomography, and transmission electron microscopy, a trilaminar structure and uniform thickness with mature, densely packed collagen fibers were seen in the ACS/bFGF group. Auditory brainstem response evaluation also showed that ACS/bFGF treatment promoted faster functional hearing recovery as compared with the control group. Conclusions: ACS is an effective TM scaffold and a carrier for bFGF. ACS/bFGF improves the TM closure rate, results in better-reconstructed TMs, and improves hearing. ACS/bFGF serves as a potential substitute for TM perforations in clinical settings.
AB - Objective: To evaluate the efficacy of acellular collagen scaffold (ACS) in combination with basic fibroblast growth factor (bFGF) for the repair of traumatic tympanic membrane (TM) perforation in a rat model. Study Design: A prospective controlled animal study in a rat model of traumatic TM perforation. Setting: Tertiary medical center. Subjects and Methods: Sprague-Dawley rats (N = 84) with unilateral traumatic perforation of the right TMs were randomized to receive ACS, bFGF, ACS in combination with bFGF (ACS/bFGF), or nothing (spontaneous healing without any interventions as a control group). The healing outcomes were evaluated by otoscopy, optical coherence tomography, histology, and transmission electron microscopy at 1, 2, and 4 weeks postoperatively. The hearing outcomes were assessed with auditory brainstem response testing. Results: ACS/bFGF resulted in higher perforation closure rates at an earlier stage than spontaneous healing, ACS, and bFGF. Based on histology, optical coherence tomography, and transmission electron microscopy, a trilaminar structure and uniform thickness with mature, densely packed collagen fibers were seen in the ACS/bFGF group. Auditory brainstem response evaluation also showed that ACS/bFGF treatment promoted faster functional hearing recovery as compared with the control group. Conclusions: ACS is an effective TM scaffold and a carrier for bFGF. ACS/bFGF improves the TM closure rate, results in better-reconstructed TMs, and improves hearing. ACS/bFGF serves as a potential substitute for TM perforations in clinical settings.
KW - basic fibroblast growth factor
KW - collagen
KW - healing
KW - hearing
KW - scaffolds
KW - tympanic membrane perforation
UR - http://www.scopus.com/inward/record.url?scp=85087880476&partnerID=8YFLogxK
U2 - 10.1177/0194599820938345
DO - 10.1177/0194599820938345
M3 - Article
C2 - 32662734
AN - SCOPUS:85087880476
VL - 164
SP - 381
EP - 390
JO - Otolaryngology - Head and neck surgery
JF - Otolaryngology - Head and neck surgery
SN - 0194-5998
IS - 2
ER -