Accumulation of endogenous peptides triggers a pathogen stress response in Arabidopsis thaliana

Beata Kmiec, Rui M.M. Branca, Oliver Berkowitz, Lu Li, Yan Wang, Monika W. Murcha, James Whelan, Janne Lehtiö, Elzbieta Glaser, Pedro F. Teixeira

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The stepwise degradation of peptides to amino acids in plant mitochondria and chloroplasts is catalyzed by a network of oligopeptidases (presequence protease PreP, organellar oligopeptidase OOP) and aminopeptidases. In the present report, we show that the lack of oligopeptidase activity in Arabidopsis thaliana results in the accumulation of endogenous free peptides, mostly of chloroplastic origin (targeting peptides and degradation products). Using mRNA sequencing and deep coverage proteomics, allowing for the identification of 17 000 transcripts and 11 000 proteins, respectively, we uncover a peptide-stress response occurring in plants lacking PreP and OOP oligopeptidase activity. The peptide-stress response results in the activation of the classical plant defense pathways in the absence of pathogenic challenge. The constitutive activation of the pathogen-defense pathways imposes a strong growth penalty and a reduction of the plants reproductive fitness. Our results indicate that the absence of organellar oligopeptidases PreP1/2 and OOP results in the accumulation of peptides that are perceived as pathogenic effectors and activate the signaling pathways of plant-defense response.

Original languageEnglish
Pages (from-to)705-715
Number of pages11
JournalPlant Journal
Volume96
Issue number4
DOIs
Publication statusPublished - 1 Nov 2018

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Arabidopsis
stress response
Arabidopsis thaliana
peptides
Peptides
pathogens
Genetic Fitness
High-Throughput Nucleotide Sequencing
Aminopeptidases
degradation
aminopeptidases
Chloroplasts
Proteomics
proteomics
Mitochondria
mitochondria
Peptide Hydrolases
proteinases
chloroplasts
oligopeptidase

Cite this

Kmiec, B., Branca, R. M. M., Berkowitz, O., Li, L., Wang, Y., Murcha, M. W., ... Teixeira, P. F. (2018). Accumulation of endogenous peptides triggers a pathogen stress response in Arabidopsis thaliana. Plant Journal, 96(4), 705-715. https://doi.org/10.1111/tpj.14100
Kmiec, Beata ; Branca, Rui M.M. ; Berkowitz, Oliver ; Li, Lu ; Wang, Yan ; Murcha, Monika W. ; Whelan, James ; Lehtiö, Janne ; Glaser, Elzbieta ; Teixeira, Pedro F. / Accumulation of endogenous peptides triggers a pathogen stress response in Arabidopsis thaliana. In: Plant Journal. 2018 ; Vol. 96, No. 4. pp. 705-715.
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Kmiec, B, Branca, RMM, Berkowitz, O, Li, L, Wang, Y, Murcha, MW, Whelan, J, Lehtiö, J, Glaser, E & Teixeira, PF 2018, 'Accumulation of endogenous peptides triggers a pathogen stress response in Arabidopsis thaliana' Plant Journal, vol. 96, no. 4, pp. 705-715. https://doi.org/10.1111/tpj.14100

Accumulation of endogenous peptides triggers a pathogen stress response in Arabidopsis thaliana. / Kmiec, Beata; Branca, Rui M.M.; Berkowitz, Oliver; Li, Lu; Wang, Yan; Murcha, Monika W.; Whelan, James; Lehtiö, Janne; Glaser, Elzbieta; Teixeira, Pedro F.

In: Plant Journal, Vol. 96, No. 4, 01.11.2018, p. 705-715.

Research output: Contribution to journalArticle

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