Accelerating the generation of homozygosity and genome fixation in pea (Pisum sativum L.)

    Research output: ThesisDoctoral Thesis

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    Abstract

    [Truncated abstract] Pea cultivars are nearly homozygous and thus homogeneous when they are released. The traditional method of selfing is slow and inefficient, taking up to ten generations of inbreeding following a cross to achieve a high level of homozygosity. Current single-seed-descent (SSD) methodologies enable a maximum of three generations per year to be developed in pea. Doubled haploidy and an in vitro based modified SSD technology have been utilised in many important crops for the rapid achievement of homozygosity, and thus acceleration of the breeding process. In pea, due to the lack of robust protocols, neither of these technologies is routinely used in a breeding program. The aim of this study was to accelerate the breeding process in pea by developing in vitro techniques to more rapidly achieve a high level of homozygosity and to gain a better understanding of the fundamental mechanisms involved in these processes. These techniques include: 1) haploidisation from cultured anthers in selected genotypes of varying backgrounds; and 2) in vitro flowering and seed-set for use in SSD breeding strategies. The development of robust genotype-independent in vitro protocols will be of great value to accelerate the breeding process in pea.
    Original languageEnglish
    QualificationDoctor of Philosophy
    Publication statusUnpublished - 2014

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