Abundance and diversity of carbon monoxide dehydrogenase genes from BMS Glade bacteria in different vegetated soils

Xiaohong Wu, Tida Ge, Yajun Hu, Xiaomeng Wei, Liang Chen, A. S. Whiteley, Jinshui Wu

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    Abstract

    Carbon monoxide (CO)-oxidizing bacteria are active consumers of atmospheric CO, undergoing a CO oxidation process that is catalyzed by carbon monoxide dehydrogenase (CODH). Little is known about the phylogeny of a group of bacterial CO-oxidizers known as the BMS Glade, which possess putative CODH genes, or their abundances in soils. In this study coxL genes indicative of bacteria belonging to the BMS Glade were amplified from DNA directly extracted from four vegetated soils: paddy rice (PR) soil, maize cropland (ML) soil, tea plantation (TP) soil, and natural forest (NF) soil. Quantitative PCR (qPCR) analysis showed that BMS coxL gene copy numbers ranged from 1.47 x 10(7) to 7.40 x 10(8) copies g(-1)dry soil and differed significantly among PR, ML, TP, and NF soils (P <0.05). CoxL gene abundance appeared to be linked to total P and Olsen P concentrations, as revealed by correlation analysis (P <0.05). Phylogenetic analysis of BMS coxL sequences indicated a diverse Glade of BMS CO oxidizers closely related to Bradyrhizobium sp. ARR65, Rhizobiales GAS188, Paraburkholderia phytofirmans, and Tistlia consotensis. However, the community compositions of BMS coxL-containing bacteria differed among soils. Collectively, these results indicate a high abundance and diversity of BMS Glade CO oxidizers in a wide variety of vegetated soils. (C) 2017 Elsevier Masson SAS. All rights reserved.

    Original languageEnglish
    Pages (from-to)94-99
    Number of pages6
    JournalEuropean Journal of Soil Biology
    Volume81
    DOIs
    Publication statusPublished - Jul 2017

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