Abundance and diversity of carbon monoxide dehydrogenase genes from BMS Glade bacteria in different vegetated soils

Xiaohong Wu; Tida Ge; Yajun Hu; Xiaomeng Wei; Liang Chen; A. S. Whiteley; Jinshui Wu

doi:10.1016/j.ejsobi.2017.06.012

Abundance and diversity of carbon monoxide dehydrogenase genes from BMS Glade bacteria in different vegetated soils

Xiaohong Wu, Tida Ge, Yajun Hu, Xiaomeng Wei, Liang Chen, A. S. Whiteley, Jinshui Wu

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Carbon monoxide (CO)-oxidizing bacteria are active consumers of atmospheric CO, undergoing a CO oxidation process that is catalyzed by carbon monoxide dehydrogenase (CODH). Little is known about the phylogeny of a group of bacterial CO-oxidizers known as the BMS Glade, which possess putative CODH genes, or their abundances in soils. In this study coxL genes indicative of bacteria belonging to the BMS Glade were amplified from DNA directly extracted from four vegetated soils: paddy rice (PR) soil, maize cropland (ML) soil, tea plantation (TP) soil, and natural forest (NF) soil. Quantitative PCR (qPCR) analysis showed that BMS coxL gene copy numbers ranged from 1.47 x 10(7) to 7.40 x 10(8) copies g(-1)dry soil and differed significantly among PR, ML, TP, and NF soils (P <0.05). CoxL gene abundance appeared to be linked to total P and Olsen P concentrations, as revealed by correlation analysis (P <0.05). Phylogenetic analysis of BMS coxL sequences indicated a diverse Glade of BMS CO oxidizers closely related to Bradyrhizobium sp. ARR65, Rhizobiales GAS188, Paraburkholderia phytofirmans, and Tistlia consotensis. However, the community compositions of BMS coxL-containing bacteria differed among soils. Collectively, these results indicate a high abundance and diversity of BMS Glade CO oxidizers in a wide variety of vegetated soils. (C) 2017 Elsevier Masson SAS. All rights reserved.

Original language	English
Pages (from-to)	94-99
Number of pages	6
Journal	European Journal of Soil Biology
Volume	81
DOIs	https://doi.org/10.1016/j.ejsobi.2017.06.012
Publication status	Published - Jul 2017

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@article{626c6e0035aa43deb91bec66b5568590,

title = "Abundance and diversity of carbon monoxide dehydrogenase genes from BMS Glade bacteria in different vegetated soils",

abstract = "Carbon monoxide (CO)-oxidizing bacteria are active consumers of atmospheric CO, undergoing a CO oxidation process that is catalyzed by carbon monoxide dehydrogenase (CODH). Little is known about the phylogeny of a group of bacterial CO-oxidizers known as the BMS Glade, which possess putative CODH genes, or their abundances in soils. In this study coxL genes indicative of bacteria belonging to the BMS Glade were amplified from DNA directly extracted from four vegetated soils: paddy rice (PR) soil, maize cropland (ML) soil, tea plantation (TP) soil, and natural forest (NF) soil. Quantitative PCR (qPCR) analysis showed that BMS coxL gene copy numbers ranged from 1.47 x 10(7) to 7.40 x 10(8) copies g(-1)dry soil and differed significantly among PR, ML, TP, and NF soils (P <0.05). CoxL gene abundance appeared to be linked to total P and Olsen P concentrations, as revealed by correlation analysis (P <0.05). Phylogenetic analysis of BMS coxL sequences indicated a diverse Glade of BMS CO oxidizers closely related to Bradyrhizobium sp. ARR65, Rhizobiales GAS188, Paraburkholderia phytofirmans, and Tistlia consotensis. However, the community compositions of BMS coxL-containing bacteria differed among soils. Collectively, these results indicate a high abundance and diversity of BMS Glade CO oxidizers in a wide variety of vegetated soils. (C) 2017 Elsevier Masson SAS. All rights reserved.",

keywords = "Carbon monoxide dehydrogenase, coxL gene, Abundance, Phylogeny, Soils, HAWAIIAN VOLCANIC DEPOSITS, LARGE SUBUNIT GENE, OXIDIZING BACTERIA, CO, QUANTIFICATION, MICROORGANISMS, HYDROGEN, LAND",

author = "Xiaohong Wu and Tida Ge and Yajun Hu and Xiaomeng Wei and Liang Chen and Whiteley, {A. S.} and Jinshui Wu",

year = "2017",

month = jul,

doi = "10.1016/j.ejsobi.2017.06.012",

language = "English",

volume = "81",

pages = "94--99",

journal = "European Journal of Soil Biology",

issn = "0035-1822",

publisher = "Elsevier",

}

TY - JOUR

T1 - Abundance and diversity of carbon monoxide dehydrogenase genes from BMS Glade bacteria in different vegetated soils

AU - Wu, Xiaohong

AU - Ge, Tida

AU - Hu, Yajun

AU - Wei, Xiaomeng

AU - Chen, Liang

AU - Whiteley, A. S.

AU - Wu, Jinshui

PY - 2017/7

Y1 - 2017/7

N2 - Carbon monoxide (CO)-oxidizing bacteria are active consumers of atmospheric CO, undergoing a CO oxidation process that is catalyzed by carbon monoxide dehydrogenase (CODH). Little is known about the phylogeny of a group of bacterial CO-oxidizers known as the BMS Glade, which possess putative CODH genes, or their abundances in soils. In this study coxL genes indicative of bacteria belonging to the BMS Glade were amplified from DNA directly extracted from four vegetated soils: paddy rice (PR) soil, maize cropland (ML) soil, tea plantation (TP) soil, and natural forest (NF) soil. Quantitative PCR (qPCR) analysis showed that BMS coxL gene copy numbers ranged from 1.47 x 10(7) to 7.40 x 10(8) copies g(-1)dry soil and differed significantly among PR, ML, TP, and NF soils (P <0.05). CoxL gene abundance appeared to be linked to total P and Olsen P concentrations, as revealed by correlation analysis (P <0.05). Phylogenetic analysis of BMS coxL sequences indicated a diverse Glade of BMS CO oxidizers closely related to Bradyrhizobium sp. ARR65, Rhizobiales GAS188, Paraburkholderia phytofirmans, and Tistlia consotensis. However, the community compositions of BMS coxL-containing bacteria differed among soils. Collectively, these results indicate a high abundance and diversity of BMS Glade CO oxidizers in a wide variety of vegetated soils. (C) 2017 Elsevier Masson SAS. All rights reserved.

AB - Carbon monoxide (CO)-oxidizing bacteria are active consumers of atmospheric CO, undergoing a CO oxidation process that is catalyzed by carbon monoxide dehydrogenase (CODH). Little is known about the phylogeny of a group of bacterial CO-oxidizers known as the BMS Glade, which possess putative CODH genes, or their abundances in soils. In this study coxL genes indicative of bacteria belonging to the BMS Glade were amplified from DNA directly extracted from four vegetated soils: paddy rice (PR) soil, maize cropland (ML) soil, tea plantation (TP) soil, and natural forest (NF) soil. Quantitative PCR (qPCR) analysis showed that BMS coxL gene copy numbers ranged from 1.47 x 10(7) to 7.40 x 10(8) copies g(-1)dry soil and differed significantly among PR, ML, TP, and NF soils (P <0.05). CoxL gene abundance appeared to be linked to total P and Olsen P concentrations, as revealed by correlation analysis (P <0.05). Phylogenetic analysis of BMS coxL sequences indicated a diverse Glade of BMS CO oxidizers closely related to Bradyrhizobium sp. ARR65, Rhizobiales GAS188, Paraburkholderia phytofirmans, and Tistlia consotensis. However, the community compositions of BMS coxL-containing bacteria differed among soils. Collectively, these results indicate a high abundance and diversity of BMS Glade CO oxidizers in a wide variety of vegetated soils. (C) 2017 Elsevier Masson SAS. All rights reserved.

KW - Carbon monoxide dehydrogenase

KW - coxL gene

KW - Abundance

KW - Phylogeny

KW - Soils

KW - HAWAIIAN VOLCANIC DEPOSITS

KW - LARGE SUBUNIT GENE

KW - OXIDIZING BACTERIA

KW - CO

KW - QUANTIFICATION

KW - MICROORGANISMS

KW - HYDROGEN

KW - LAND

U2 - 10.1016/j.ejsobi.2017.06.012

DO - 10.1016/j.ejsobi.2017.06.012

M3 - Article

VL - 81

SP - 94

EP - 99

JO - European Journal of Soil Biology

JF - European Journal of Soil Biology

SN - 0035-1822

ER -

Abundance and diversity of carbon monoxide dehydrogenase genes from BMS Glade bacteria in different vegetated soils

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