Absorption of foliar-applied Zn in sunflower (Helianthus annuus): importance of the cuticle, stomata and trichomes

Cui Li, Peng Wang, Antony van der Ent, Miaomiao Cheng, Haibo Jiang, Thea Lund Read, Enzo Lombi, Caixian Tang, Martin D. de Jonge, Neal W. Menzies, Peter M. Kopittke

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background and Aims The pathways whereby foliar-applied nutrients move across the leaf surface remain unclear. The aim of the present study was to examine the pathways by which foliar-applied Zn moves across the sunflower (Helianthus annuus) leaf surface, considering the potential importance of the cuticle, stomata and trichomes.

Methods Using synchrotron-based X-ray florescence microscopy and nanoscale secondary ion mass spectrometry (NanoSIMS), the absorption of foliar-applied ZnSO4 and nano-ZnO were studied in sunflower. The speciation of Zn was also examined using synchrotron-based X-ray absorption spectroscopy.

Key Results Non-glandular trichomes (NGTs) were particularly important for foliar Zn absorption, with Zn preferentially accumulating within trichomes in 15 min. The cuticle was also found to have a role, with Zn appearing to move across the cuticle before accumulating in the walls of the epidermal cells. After 6 h, the total Zn that accumulated in the NGTs was approx. 1.9 times higher than in the cuticular tissues. No marked accumulation of Zn was found within the stomatal cavity, probably indicating a limited contribution of the stomatal pathway. Once absorbed, the Zn accumulated in the walls of the epidermal and the vascular cells, and trichome bases of both leaf sides, with the bundle sheath extensions that connected to the trichomes seemingly facilitating this translocation. Finally, the absorption of nano-ZnO was substantially lower than for ZnSO4, with Zn probably moving across the leaf surface as soluble Zn rather than nanoparticles.

Conclusions In sunflower, both the trichomes and cuticle appear to be important for foliar Zn absorption.

Original languageEnglish
Pages (from-to)57-68
Number of pages12
JournalAnnals of Botany
Volume123
Issue number1
DOIs
Publication statusPublished - 1 Jan 2019

Cite this

Li, Cui ; Wang, Peng ; van der Ent, Antony ; Cheng, Miaomiao ; Jiang, Haibo ; Read, Thea Lund ; Lombi, Enzo ; Tang, Caixian ; de Jonge, Martin D. ; Menzies, Neal W. ; Kopittke, Peter M. / Absorption of foliar-applied Zn in sunflower (Helianthus annuus) : importance of the cuticle, stomata and trichomes. In: Annals of Botany. 2019 ; Vol. 123, No. 1. pp. 57-68.
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title = "Absorption of foliar-applied Zn in sunflower (Helianthus annuus): importance of the cuticle, stomata and trichomes",
abstract = "Background and Aims The pathways whereby foliar-applied nutrients move across the leaf surface remain unclear. The aim of the present study was to examine the pathways by which foliar-applied Zn moves across the sunflower (Helianthus annuus) leaf surface, considering the potential importance of the cuticle, stomata and trichomes.Methods Using synchrotron-based X-ray florescence microscopy and nanoscale secondary ion mass spectrometry (NanoSIMS), the absorption of foliar-applied ZnSO4 and nano-ZnO were studied in sunflower. The speciation of Zn was also examined using synchrotron-based X-ray absorption spectroscopy.Key Results Non-glandular trichomes (NGTs) were particularly important for foliar Zn absorption, with Zn preferentially accumulating within trichomes in 15 min. The cuticle was also found to have a role, with Zn appearing to move across the cuticle before accumulating in the walls of the epidermal cells. After 6 h, the total Zn that accumulated in the NGTs was approx. 1.9 times higher than in the cuticular tissues. No marked accumulation of Zn was found within the stomatal cavity, probably indicating a limited contribution of the stomatal pathway. Once absorbed, the Zn accumulated in the walls of the epidermal and the vascular cells, and trichome bases of both leaf sides, with the bundle sheath extensions that connected to the trichomes seemingly facilitating this translocation. Finally, the absorption of nano-ZnO was substantially lower than for ZnSO4, with Zn probably moving across the leaf surface as soluble Zn rather than nanoparticles.Conclusions In sunflower, both the trichomes and cuticle appear to be important for foliar Zn absorption.",
keywords = "Bundle sheath extension, cuticle, foliar absorption, stomata, trichomes, Zn foliar fertilizer, sunflower, WATER PERMEABILITY, PLANT CUTICLES, LEAVES, PENETRATION, SPECIATION, MANGANESE, ZINC, NANOTECHNOLOGY, ACCUMULATION, MECHANISMS",
author = "Cui Li and Peng Wang and {van der Ent}, Antony and Miaomiao Cheng and Haibo Jiang and Read, {Thea Lund} and Enzo Lombi and Caixian Tang and {de Jonge}, {Martin D.} and Menzies, {Neal W.} and Kopittke, {Peter M.}",
year = "2019",
month = "1",
day = "1",
doi = "10.1093/aob/mcy135",
language = "English",
volume = "123",
pages = "57--68",
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Li, C, Wang, P, van der Ent, A, Cheng, M, Jiang, H, Read, TL, Lombi, E, Tang, C, de Jonge, MD, Menzies, NW & Kopittke, PM 2019, 'Absorption of foliar-applied Zn in sunflower (Helianthus annuus): importance of the cuticle, stomata and trichomes' Annals of Botany, vol. 123, no. 1, pp. 57-68. https://doi.org/10.1093/aob/mcy135

Absorption of foliar-applied Zn in sunflower (Helianthus annuus) : importance of the cuticle, stomata and trichomes. / Li, Cui; Wang, Peng; van der Ent, Antony; Cheng, Miaomiao; Jiang, Haibo; Read, Thea Lund; Lombi, Enzo; Tang, Caixian; de Jonge, Martin D.; Menzies, Neal W.; Kopittke, Peter M.

In: Annals of Botany, Vol. 123, No. 1, 01.01.2019, p. 57-68.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Absorption of foliar-applied Zn in sunflower (Helianthus annuus)

T2 - importance of the cuticle, stomata and trichomes

AU - Li, Cui

AU - Wang, Peng

AU - van der Ent, Antony

AU - Cheng, Miaomiao

AU - Jiang, Haibo

AU - Read, Thea Lund

AU - Lombi, Enzo

AU - Tang, Caixian

AU - de Jonge, Martin D.

AU - Menzies, Neal W.

AU - Kopittke, Peter M.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Background and Aims The pathways whereby foliar-applied nutrients move across the leaf surface remain unclear. The aim of the present study was to examine the pathways by which foliar-applied Zn moves across the sunflower (Helianthus annuus) leaf surface, considering the potential importance of the cuticle, stomata and trichomes.Methods Using synchrotron-based X-ray florescence microscopy and nanoscale secondary ion mass spectrometry (NanoSIMS), the absorption of foliar-applied ZnSO4 and nano-ZnO were studied in sunflower. The speciation of Zn was also examined using synchrotron-based X-ray absorption spectroscopy.Key Results Non-glandular trichomes (NGTs) were particularly important for foliar Zn absorption, with Zn preferentially accumulating within trichomes in 15 min. The cuticle was also found to have a role, with Zn appearing to move across the cuticle before accumulating in the walls of the epidermal cells. After 6 h, the total Zn that accumulated in the NGTs was approx. 1.9 times higher than in the cuticular tissues. No marked accumulation of Zn was found within the stomatal cavity, probably indicating a limited contribution of the stomatal pathway. Once absorbed, the Zn accumulated in the walls of the epidermal and the vascular cells, and trichome bases of both leaf sides, with the bundle sheath extensions that connected to the trichomes seemingly facilitating this translocation. Finally, the absorption of nano-ZnO was substantially lower than for ZnSO4, with Zn probably moving across the leaf surface as soluble Zn rather than nanoparticles.Conclusions In sunflower, both the trichomes and cuticle appear to be important for foliar Zn absorption.

AB - Background and Aims The pathways whereby foliar-applied nutrients move across the leaf surface remain unclear. The aim of the present study was to examine the pathways by which foliar-applied Zn moves across the sunflower (Helianthus annuus) leaf surface, considering the potential importance of the cuticle, stomata and trichomes.Methods Using synchrotron-based X-ray florescence microscopy and nanoscale secondary ion mass spectrometry (NanoSIMS), the absorption of foliar-applied ZnSO4 and nano-ZnO were studied in sunflower. The speciation of Zn was also examined using synchrotron-based X-ray absorption spectroscopy.Key Results Non-glandular trichomes (NGTs) were particularly important for foliar Zn absorption, with Zn preferentially accumulating within trichomes in 15 min. The cuticle was also found to have a role, with Zn appearing to move across the cuticle before accumulating in the walls of the epidermal cells. After 6 h, the total Zn that accumulated in the NGTs was approx. 1.9 times higher than in the cuticular tissues. No marked accumulation of Zn was found within the stomatal cavity, probably indicating a limited contribution of the stomatal pathway. Once absorbed, the Zn accumulated in the walls of the epidermal and the vascular cells, and trichome bases of both leaf sides, with the bundle sheath extensions that connected to the trichomes seemingly facilitating this translocation. Finally, the absorption of nano-ZnO was substantially lower than for ZnSO4, with Zn probably moving across the leaf surface as soluble Zn rather than nanoparticles.Conclusions In sunflower, both the trichomes and cuticle appear to be important for foliar Zn absorption.

KW - Bundle sheath extension

KW - cuticle

KW - foliar absorption

KW - stomata

KW - trichomes

KW - Zn foliar fertilizer

KW - sunflower

KW - WATER PERMEABILITY

KW - PLANT CUTICLES

KW - LEAVES

KW - PENETRATION

KW - SPECIATION

KW - MANGANESE

KW - ZINC

KW - NANOTECHNOLOGY

KW - ACCUMULATION

KW - MECHANISMS

U2 - 10.1093/aob/mcy135

DO - 10.1093/aob/mcy135

M3 - Article

VL - 123

SP - 57

EP - 68

JO - Annals of Botany

JF - Annals of Botany

SN - 0305-7364

IS - 1

ER -