Absence of cholinergic airway tone in normal BALB/c mice

Alexander Larcombe; Graeme Zosky; E.M. Bozanich; Debra Turner; Zoltan Hantos; Peter Sly

doi:10.1016/j.resp.2008.01.009

Absence of cholinergic airway tone in normal BALB/c mice

Alexander Larcombe, Graeme Zosky, E.M. Bozanich, Debra Turner, Zoltan Hantos, Peter Sly

Research output: Contribution to journal › Article

7 Citations (Scopus)

Abstract

Basal airway smooth muscle (ASM) tone has not been demonstrated in mice in vivo. To determine whether basal ASM tone is present in mouse airways we measured respiratory system impedance (Zrs) before and after either atropine or bilateral vagotomy. Zrs was measured using forced oscillations delivered via a wave-tube during slow (similar to 35 s) inflation-deflation maneuvers between transrespiratory pressures (Prs) of 0 and 20 cm H2O. A constant-phase tissue model was applied to the Zrs to calculate airway resistance (R-aw), tissue damping (G) and elastance (H). Thoracic gas volume (TGV) was determined plethysmographically at Prs = 0 cm H2O and by integration of the inspiratory flow. The relationship between conductance (G(aw) = l/R-aw) and TGV during inflation was also examined. Neither atropine nor vagotomy produced any change in R-aw, H, eta (=G/H), TGV or the slope of G(aw) vs. TGV that was different to that observed in the relevant control groups. These data show that BALB/c mice do not have cholinergic ASM tone in vivo. (C) 2008 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	223-229
Journal	Respiratory Physiology & Neurobiology
Volume	161
Issue number	3
DOIs	https://doi.org/10.1016/j.resp.2008.01.009
Publication status	Published - 2008

Access to Document

10.1016/j.resp.2008.01.009

Fingerprint Dive into the research topics of 'Absence of cholinergic airway tone in normal BALB/c mice'. Together they form a unique fingerprint.

Cite this

@article{6929efffa03e4dfa9b232e5976023a8b,

title = "Absence of cholinergic airway tone in normal BALB/c mice",

abstract = "Basal airway smooth muscle (ASM) tone has not been demonstrated in mice in vivo. To determine whether basal ASM tone is present in mouse airways we measured respiratory system impedance (Zrs) before and after either atropine or bilateral vagotomy. Zrs was measured using forced oscillations delivered via a wave-tube during slow (similar to 35 s) inflation-deflation maneuvers between transrespiratory pressures (Prs) of 0 and 20 cm H2O. A constant-phase tissue model was applied to the Zrs to calculate airway resistance (R-aw), tissue damping (G) and elastance (H). Thoracic gas volume (TGV) was determined plethysmographically at Prs = 0 cm H2O and by integration of the inspiratory flow. The relationship between conductance (G(aw) = l/R-aw) and TGV during inflation was also examined. Neither atropine nor vagotomy produced any change in R-aw, H, eta (=G/H), TGV or the slope of G(aw) vs. TGV that was different to that observed in the relevant control groups. These data show that BALB/c mice do not have cholinergic ASM tone in vivo. (C) 2008 Elsevier B.V. All rights reserved.",

author = "Alexander Larcombe and Graeme Zosky and E.M. Bozanich and Debra Turner and Zoltan Hantos and Peter Sly",

year = "2008",

doi = "10.1016/j.resp.2008.01.009",

language = "English",

volume = "161",

pages = "223--229",

journal = "Respiratory Physiology & Neurobiology",

issn = "1569-9048",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Absence of cholinergic airway tone in normal BALB/c mice

AU - Larcombe, Alexander

AU - Zosky, Graeme

AU - Bozanich, E.M.

AU - Turner, Debra

AU - Hantos, Zoltan

AU - Sly, Peter

PY - 2008

Y1 - 2008

N2 - Basal airway smooth muscle (ASM) tone has not been demonstrated in mice in vivo. To determine whether basal ASM tone is present in mouse airways we measured respiratory system impedance (Zrs) before and after either atropine or bilateral vagotomy. Zrs was measured using forced oscillations delivered via a wave-tube during slow (similar to 35 s) inflation-deflation maneuvers between transrespiratory pressures (Prs) of 0 and 20 cm H2O. A constant-phase tissue model was applied to the Zrs to calculate airway resistance (R-aw), tissue damping (G) and elastance (H). Thoracic gas volume (TGV) was determined plethysmographically at Prs = 0 cm H2O and by integration of the inspiratory flow. The relationship between conductance (G(aw) = l/R-aw) and TGV during inflation was also examined. Neither atropine nor vagotomy produced any change in R-aw, H, eta (=G/H), TGV or the slope of G(aw) vs. TGV that was different to that observed in the relevant control groups. These data show that BALB/c mice do not have cholinergic ASM tone in vivo. (C) 2008 Elsevier B.V. All rights reserved.

AB - Basal airway smooth muscle (ASM) tone has not been demonstrated in mice in vivo. To determine whether basal ASM tone is present in mouse airways we measured respiratory system impedance (Zrs) before and after either atropine or bilateral vagotomy. Zrs was measured using forced oscillations delivered via a wave-tube during slow (similar to 35 s) inflation-deflation maneuvers between transrespiratory pressures (Prs) of 0 and 20 cm H2O. A constant-phase tissue model was applied to the Zrs to calculate airway resistance (R-aw), tissue damping (G) and elastance (H). Thoracic gas volume (TGV) was determined plethysmographically at Prs = 0 cm H2O and by integration of the inspiratory flow. The relationship between conductance (G(aw) = l/R-aw) and TGV during inflation was also examined. Neither atropine nor vagotomy produced any change in R-aw, H, eta (=G/H), TGV or the slope of G(aw) vs. TGV that was different to that observed in the relevant control groups. These data show that BALB/c mice do not have cholinergic ASM tone in vivo. (C) 2008 Elsevier B.V. All rights reserved.

U2 - 10.1016/j.resp.2008.01.009

DO - 10.1016/j.resp.2008.01.009

M3 - Article

VL - 161

SP - 223

EP - 229

JO - Respiratory Physiology & Neurobiology

JF - Respiratory Physiology & Neurobiology

SN - 1569-9048

IS - 3

ER -