Abnormal neutrophils in acute myeloid leukemia and myelodysplastic syndrome

Frederick R. Davey, Wendy N. Erber, Kevin C. Gatter, David Y. Mason

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Neutrophils and band forms from patients with acute myeloid leukemia and myelodysplastic syndrome were stained for the presence of myeloperoxidase using a cytochemical method (diaminobenzidine/hydrogen peroxide) and the alkaline phosphatase-anti-alkaline phosphatase immunocytochemical procedure (using monoclonal anti-myeloperoxidase). Neutrophils and bands were also stained for elastase and lactoferrin using monoclonal and polyclonal antibodies, respectively. Subpopulations of neutrophils and bands from cases of acute myeloid leukemia and myelodysplasia exhibited a qualitative and/or quantitative deficiency in myeloperoxidase. In addition, a quantitative decrease in elastase and/or lactoferrin staining was detected. Thus, neutrophils and bands from patients with acute myoloid leukemia and myelodysplastic syndrome have a defect in one or more of the constituents of primary and/or secondary granules. These defects are consistent with the view that abnormal neutrophils and bands are derived from a malignant clone of myeloid precursor cells.

Original languageEnglish
Pages (from-to)454-459
Number of pages6
JournalHuman Pathology
Volume19
Issue number4
DOIs
Publication statusPublished - 1 Jan 1988
Externally publishedYes

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Myelodysplastic Syndromes
Acute Myeloid Leukemia
Neutrophils
Lactoferrin
Pancreatic Elastase
Peroxidase
Alkaline Phosphatase
Myeloid Cells
Hydrogen Peroxide
Leukemia
Clone Cells
Monoclonal Antibodies
Staining and Labeling

Cite this

Davey, Frederick R. ; Erber, Wendy N. ; Gatter, Kevin C. ; Mason, David Y. / Abnormal neutrophils in acute myeloid leukemia and myelodysplastic syndrome. In: Human Pathology. 1988 ; Vol. 19, No. 4. pp. 454-459.
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Abnormal neutrophils in acute myeloid leukemia and myelodysplastic syndrome. / Davey, Frederick R.; Erber, Wendy N.; Gatter, Kevin C.; Mason, David Y.

In: Human Pathology, Vol. 19, No. 4, 01.01.1988, p. 454-459.

Research output: Contribution to journalArticle

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AU - Davey, Frederick R.

AU - Erber, Wendy N.

AU - Gatter, Kevin C.

AU - Mason, David Y.

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AB - Neutrophils and band forms from patients with acute myeloid leukemia and myelodysplastic syndrome were stained for the presence of myeloperoxidase using a cytochemical method (diaminobenzidine/hydrogen peroxide) and the alkaline phosphatase-anti-alkaline phosphatase immunocytochemical procedure (using monoclonal anti-myeloperoxidase). Neutrophils and bands were also stained for elastase and lactoferrin using monoclonal and polyclonal antibodies, respectively. Subpopulations of neutrophils and bands from cases of acute myeloid leukemia and myelodysplasia exhibited a qualitative and/or quantitative deficiency in myeloperoxidase. In addition, a quantitative decrease in elastase and/or lactoferrin staining was detected. Thus, neutrophils and bands from patients with acute myoloid leukemia and myelodysplastic syndrome have a defect in one or more of the constituents of primary and/or secondary granules. These defects are consistent with the view that abnormal neutrophils and bands are derived from a malignant clone of myeloid precursor cells.

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