TY - JOUR
T1 - A Validated High-Performance Thin-Layer Chromatography Method for Analyzing Fat-Soluble Vitamins in Commercial Pharmaceutical Preparations
AU - Sikdar, K M Yasif Kayes
AU - Islam, Khairul
AU - Sostaric, Tom
AU - Lim, Lee Yong
AU - Locher, Connie
PY - 2024/12
Y1 - 2024/12
N2 - A simple, cost-effective, and efficient novel high-performance thin-layer chromatography (HPTLC) tool was developed and validated in accordance with International Conference on Harmonisation (ICH) guidelines for the detection and quantification of four fat-soluble vitamins, D2, D3, E, and K1, using chloroform: cyclohexane (55:45, v/v) as mobile phase. The detection and quantification limits were found to be 30.86 and 93.52 ng/band for vitamin D2, 19.44 and 58.92 ng/band for vitamin D3, 14.17 and 42.95 ng/band for vitamin E, and 0.86 and 2.61 ng/band for vitamin K1, which were similar or lower than those reported in previous methods. The advantage of the developed method is that it uses a simple mobile phase in a single development step and has low detection and quantification limits. The application of the developed HPTLC method was successfully demonstrated with the quantitative analysis of these vitamins in some commercially available pharmaceutical preparations.
AB - A simple, cost-effective, and efficient novel high-performance thin-layer chromatography (HPTLC) tool was developed and validated in accordance with International Conference on Harmonisation (ICH) guidelines for the detection and quantification of four fat-soluble vitamins, D2, D3, E, and K1, using chloroform: cyclohexane (55:45, v/v) as mobile phase. The detection and quantification limits were found to be 30.86 and 93.52 ng/band for vitamin D2, 19.44 and 58.92 ng/band for vitamin D3, 14.17 and 42.95 ng/band for vitamin E, and 0.86 and 2.61 ng/band for vitamin K1, which were similar or lower than those reported in previous methods. The advantage of the developed method is that it uses a simple mobile phase in a single development step and has low detection and quantification limits. The application of the developed HPTLC method was successfully demonstrated with the quantitative analysis of these vitamins in some commercially available pharmaceutical preparations.
KW - High-performance thin-layer chromatography (HPTLC)
KW - Validation
KW - Fat-soluble vitamins
KW - Quantification
KW - Pharmaceutical preparations
U2 - 10.3390/app142311064
DO - 10.3390/app142311064
M3 - Article
SN - 2076-3417
VL - 14
JO - Applied Sciences
JF - Applied Sciences
IS - 23
M1 - 11064
ER -