A Val-202-Phe α-tubulin mutation and enhanced metabolism confer dinitroaniline resistance in a single Lolium rigidum population

Jinyi Chen, Zhizhan Chu, Heping Han, Danica E. Goggin, Qin Yu, Chad Sayer, Stephen B. Powles

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

BACKGROUND: A Lolium rigidum population collected from Western Australia was previously reported as highly resistant to dinitroaniline herbicides mainly due to a Val-202-Phe substitution in the target site α-tubulin protein. To further determine the contribution of the 202 mutation to resistance, two sub-populations, respectively comprising the 202 mutant and wild-type (WT) individuals, were isolated from within the same resistant population and subject to dinitroaniline herbicide doses. A rice transgenic study was conducted to demonstrate whether the amino acid substitution at the 202 residue confers resistance. In addition, as indicated in the phenotyping and genotyping study, non-target enhanced trifluralin metabolism was further examined in the same population. RESULTS: The 202 mutants were more resistant than the wild-type plants. Rice calli transformed with the L. rigidum mutant α-tubulin gene (Val-202-Phe) were more resistant to dinitroaniline herbicides relative to calli transformed with the wild-type gene. Also, enhanced trifluralin metabolism was detected in the 202 mutants in comparison to the susceptible seedlings. CONLCUSION: Both target-site Val-202-Phe α-tubulin mutation and non-target-site enhanced trifluralin metabolism co-exist in this dinitroaniline-resistant L. rigidum population.

Original languageEnglish
Pages (from-to)645-652
Number of pages8
JournalPest Management Science
Volume76
Issue number2
Early online date22 Jul 2019
DOIs
Publication statusPublished - Feb 2020

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