A Val-202-Phe α-tubulin mutation and enhanced metabolism confer dinitroaniline resistance in a single Lolium rigidum population

Jinyi Chen, Zhizhan Chu, Heping Han, Danica E. Goggin, Qin Yu, Chad Sayer, Stephen B. Powles

Research output: Contribution to journalArticle

Abstract

BACKGROUND: A Lolium rigidum population collected from Western Australia was previously reported as highly resistant to dinitroaniline herbicides mainly due to a Val-202-Phe substitution in the target site α-tubulin protein. To further determine the contribution of the 202 mutation to resistance, two sub-populations, respectively comprising the 202 mutant and wild-type (WT) individuals, were isolated from within the same resistant population and subject to dinitroaniline herbicide doses. A rice transgenic study was conducted to demonstrate whether the amino acid substitution at the 202 residue confers resistance. In addition, as indicated in the phenotyping and genotyping study, non-target enhanced trifluralin metabolism was further examined in the same population. RESULTS: The 202 mutants were more resistant than the wild-type plants. Rice calli transformed with the L. rigidum mutant α-tubulin gene (Val-202-Phe) were more resistant to dinitroaniline herbicides relative to calli transformed with the wild-type gene. Also, enhanced trifluralin metabolism was detected in the 202 mutants in comparison to the susceptible seedlings. CONLCUSION: Both target-site Val-202-Phe α-tubulin mutation and non-target-site enhanced trifluralin metabolism co-exist in this dinitroaniline-resistant L. rigidum population.

Original languageEnglish
Number of pages8
JournalPest Management Science
DOIs
Publication statusPublished - 22 Jul 2019

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Lolium rigidum
Lolium
Tubulin
tubulin
Trifluralin
dinitroaniline herbicides
trifluralin
mutation
Herbicides
Mutation
metabolism
mutants
Population
Bony Callus
callus
rice
Western Australia
amino acid substitution
Amino Acid Substitution
Seedlings

Cite this

@article{b6799f2b42ad4a81b5954c6d7102b6f2,
title = "A Val-202-Phe α-tubulin mutation and enhanced metabolism confer dinitroaniline resistance in a single Lolium rigidum population",
abstract = "BACKGROUND: A Lolium rigidum population collected from Western Australia was previously reported as highly resistant to dinitroaniline herbicides mainly due to a Val-202-Phe substitution in the target site α-tubulin protein. To further determine the contribution of the 202 mutation to resistance, two sub-populations, respectively comprising the 202 mutant and wild-type (WT) individuals, were isolated from within the same resistant population and subject to dinitroaniline herbicide doses. A rice transgenic study was conducted to demonstrate whether the amino acid substitution at the 202 residue confers resistance. In addition, as indicated in the phenotyping and genotyping study, non-target enhanced trifluralin metabolism was further examined in the same population. RESULTS: The 202 mutants were more resistant than the wild-type plants. Rice calli transformed with the L. rigidum mutant α-tubulin gene (Val-202-Phe) were more resistant to dinitroaniline herbicides relative to calli transformed with the wild-type gene. Also, enhanced trifluralin metabolism was detected in the 202 mutants in comparison to the susceptible seedlings. CONLCUSION: Both target-site Val-202-Phe α-tubulin mutation and non-target-site enhanced trifluralin metabolism co-exist in this dinitroaniline-resistant L. rigidum population.",
keywords = "dinitroaniline, Lolium rigidum, mutation, resistance, tubulin",
author = "Jinyi Chen and Zhizhan Chu and Heping Han and Goggin, {Danica E.} and Qin Yu and Chad Sayer and Powles, {Stephen B.}",
year = "2019",
month = "7",
day = "22",
doi = "10.1002/ps.5561",
language = "English",
journal = "Pest Management Science",
issn = "1526-498X",
publisher = "John Wiley & Sons",

}

TY - JOUR

T1 - A Val-202-Phe α-tubulin mutation and enhanced metabolism confer dinitroaniline resistance in a single Lolium rigidum population

AU - Chen, Jinyi

AU - Chu, Zhizhan

AU - Han, Heping

AU - Goggin, Danica E.

AU - Yu, Qin

AU - Sayer, Chad

AU - Powles, Stephen B.

PY - 2019/7/22

Y1 - 2019/7/22

N2 - BACKGROUND: A Lolium rigidum population collected from Western Australia was previously reported as highly resistant to dinitroaniline herbicides mainly due to a Val-202-Phe substitution in the target site α-tubulin protein. To further determine the contribution of the 202 mutation to resistance, two sub-populations, respectively comprising the 202 mutant and wild-type (WT) individuals, were isolated from within the same resistant population and subject to dinitroaniline herbicide doses. A rice transgenic study was conducted to demonstrate whether the amino acid substitution at the 202 residue confers resistance. In addition, as indicated in the phenotyping and genotyping study, non-target enhanced trifluralin metabolism was further examined in the same population. RESULTS: The 202 mutants were more resistant than the wild-type plants. Rice calli transformed with the L. rigidum mutant α-tubulin gene (Val-202-Phe) were more resistant to dinitroaniline herbicides relative to calli transformed with the wild-type gene. Also, enhanced trifluralin metabolism was detected in the 202 mutants in comparison to the susceptible seedlings. CONLCUSION: Both target-site Val-202-Phe α-tubulin mutation and non-target-site enhanced trifluralin metabolism co-exist in this dinitroaniline-resistant L. rigidum population.

AB - BACKGROUND: A Lolium rigidum population collected from Western Australia was previously reported as highly resistant to dinitroaniline herbicides mainly due to a Val-202-Phe substitution in the target site α-tubulin protein. To further determine the contribution of the 202 mutation to resistance, two sub-populations, respectively comprising the 202 mutant and wild-type (WT) individuals, were isolated from within the same resistant population and subject to dinitroaniline herbicide doses. A rice transgenic study was conducted to demonstrate whether the amino acid substitution at the 202 residue confers resistance. In addition, as indicated in the phenotyping and genotyping study, non-target enhanced trifluralin metabolism was further examined in the same population. RESULTS: The 202 mutants were more resistant than the wild-type plants. Rice calli transformed with the L. rigidum mutant α-tubulin gene (Val-202-Phe) were more resistant to dinitroaniline herbicides relative to calli transformed with the wild-type gene. Also, enhanced trifluralin metabolism was detected in the 202 mutants in comparison to the susceptible seedlings. CONLCUSION: Both target-site Val-202-Phe α-tubulin mutation and non-target-site enhanced trifluralin metabolism co-exist in this dinitroaniline-resistant L. rigidum population.

KW - dinitroaniline

KW - Lolium rigidum

KW - mutation

KW - resistance

KW - tubulin

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U2 - 10.1002/ps.5561

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