A tripartite approach identifies the major sunflower seed albumins.

Achala S. Jayasena, Bastian Franke, K. Johan Rosengren, Joshua S Mylne

Research output: Contribution to journalArticle

8 Citations (Scopus)
150 Downloads (Pure)

Abstract

KEY MESSAGE: We have used a combination of genomic, transcriptomic, and proteomic approaches to identify the napin-type albumin genes in sunflower and define their contributions to the seed albumin pool. Seed protein content is determined by the expression of what are typically large gene families. A major class of seed storage proteins is the napin-type, water soluble albumins. In this work we provide a comprehensive analysis of the napin-type albumin content of the common sunflower (Helianthus annuus) by analyzing a draft genome, a transcriptome and performing a proteomic analysis of the seed albumin fraction. We show that although sunflower contains at least 26 genes for napin-type albumins, only 15 of these are present at the mRNA level. We found protein evidence for 11 of these but the albumin content of mature seeds is dominated by the encoded products of just three genes. So despite high genetic redundancy for albumins, only a small sub-set of this gene family contributes to total seed albumin content. The three genes identified as producing the majority of sunflower seed albumin are potential future candidates for manipulation through genetics and breeding.
Original languageEnglish
Pages (from-to)613–629
JournalTheoretical and Applied Genetics
Volume129
Issue number3
DOIs
Publication statusPublished - Mar 2016

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sunflower seed
Helianthus
albumins
Albumins
Seeds
Helianthus annuus
Genes
seeds
genes
Proteomics
proteomics
Seed Storage Proteins
seed storage proteins
transcriptomics
genetic engineering
Transcriptome
transcriptome
Breeding
Proteins
protein content

Cite this

Jayasena, Achala S. ; Franke, Bastian ; Rosengren, K. Johan ; Mylne, Joshua S. / A tripartite approach identifies the major sunflower seed albumins. In: Theoretical and Applied Genetics. 2016 ; Vol. 129, No. 3. pp. 613–629.
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A tripartite approach identifies the major sunflower seed albumins. / Jayasena, Achala S.; Franke, Bastian; Rosengren, K. Johan; Mylne, Joshua S.

In: Theoretical and Applied Genetics, Vol. 129, No. 3, 03.2016, p. 613–629.

Research output: Contribution to journalArticle

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