A transient disruption of fibroblastic transcriptional regulatory network facilitates trans-differentiation

Yasuhiro Tomaru, Ryota Hasegawa, Takahiro Suzuki, Taiji Sato, Atsutaka Kubosaki, Masanori Suzuki, Hideya Kawaji, Alistair R.R. Forrest, Yoshihide Hayashizaki, Jay W. Shin, Harukazu Suzuki

Research output: Contribution to journalArticle

15 Citations (Scopus)


Transcriptional Regulatory Networks (TRNs) coordinate multiple transcription factors (TFs) in concert to maintain tissue homeostasis and cellular function. The re-establishment of target cell TRNs has been previously implicated in direct trans-differentiation studies where the newly introduced TFs switch on a set of key regulatory factors to induce de novo expression and function. However, the extent to which TRNs in starting cell types, such as dermal fibroblasts, protect cells from undergoing cellular reprogramming remains largely unexplored. In order to identify TFs specific to maintaining the fibroblast state, we performed systematic knockdown of 18 fibroblast-enriched TFs and analyzed differential mRNA expression against the same 18 genes, building a Matrix-RNAi. The resulting expression matrix revealed seven highly interconnected TFs. Interestingly, suppressing four out of seven TFs generated lipid droplets and induced PPARG and CEBPA expression in the presence of adipocyte-inducing medium only, while negative control knockdown cells maintained fibroblastic character in the same induction regime. Global gene expression analyses further revealed that the knockdown-induced adipocytes expressed genes associated with lipid metabolism and significantly suppressed fibroblast genes. Overall, this study reveals the critical role of the TRN in protecting cells against aberrant reprogramming, and demonstrates the vulnerability of donor cell's TRNs, offering a novel strategy to induce transgene-free trans-differentiations.

Original languageEnglish
Pages (from-to)8905-8913
Number of pages9
JournalNucleic Acids Research
Issue number14
Publication statusPublished - 2014
Externally publishedYes

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