A systems immunology approach to understanding the development of otitis media in otitis prone children

Sharon Clark

doi:10.26182/pewz-th32

A systems immunology approach to understanding the development of otitis media in otitis prone children

Sharon Clark

UWA Medical School

Research output: Thesis › Doctoral Thesis

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Abstract

Chronic otitis media (OM) affects many children particularly Australian Aboriginal children and is predominantly caused by non-typeable Haemophilus influenzae (NTHi) within biofilms. Innate and adaptive immune responses to NTHi biofilm-associated proteins rsPilA, Protein D and ChimV4 in Aboriginal and non-Aboriginal otitis-prone children were assessed in comparison to non-otitis-prone children. Reduced humoral responses to rsPilA, PD and ChimV4 antigens were seen for both serumIgG and memory B-cell frequency but not non-biofilm related protein OMP26. Reduced Th17 and increased Th2 cytokine responses to PD, increased global frequencies of cytotoxic T-cells, Tfh cells,NK cells, monocytes and dendritic cells were also noted.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	The University of Western Australia
Supervisors/Advisors	Thornton, Ruth, Supervisor Seppanen, Elke, Supervisor Richmond, Peter, Supervisor
Thesis sponsors	Australian Government Research Training Program
Award date	27 Mar 2024
DOIs	https://doi.org/10.26182/pewz-th32
Publication status	Unpublished - 2023

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.26182/pewz-th32

THESIS - DOCTOR OF PHILOSOPHY - CLARK Sharon Louise - 2023
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6 Article
1 Letter

Egg‐sensitised infants have elevated CD4+ effector memory T regulatory cells from birth
Gamez, C., Leffler, J., Clark, S., Corscadden, K., Prescott, S. L., Palmer, D. J. & Strickland, D., Jan 2024, In: Clinical & Experimental Allergy. 54, 1, p. 34-45 12 p.
Research output: Contribution to journal › Article › peer-review

Open Access
Biofilms and intracellular infection in otitis media
Thornton, R., Seppanen, E. & Clark, S., 2023, In: Microbiology Australia. 44, 2, p. 88-91 4 p.
Research output: Contribution to journal › Article › peer-review

Open Access
Australian Aboriginal Otitis-Prone Children Produce High-Quality Serum IgG to Putative Nontypeable Haemophilus influenzae Vaccine Antigens at Lower Titres Compared to Non-Aboriginal Children
Clark, S. L., Seppanen, E. J., Kirkham, L. A. S., Novotny, L. A., Bakaletz, L. O., Cripps, A. W., Corscadden, K., Coates, H., Vijayasekaran, S., Richmond, P. C. & Thornton, R. B., 7 Apr 2022, In: Frontiers in Cellular and Infection Microbiology. 12, 767083.
Research output: Contribution to journal › Article › peer-review

Open Access
5 Citations (Web of Science)

Cite this

@phdthesis{9104723ec1544e75b0807e5c6c1600db,

title = "A systems immunology approach to understanding the development of otitis media in otitis prone children",

abstract = "Chronic otitis media (OM) affects many children particularly Australian Aboriginal children and is predominantly caused by non-typeable Haemophilus influenzae (NTHi) within biofilms. Innate and adaptive immune responses to NTHi biofilm-associated proteins rsPilA, Protein D and ChimV4 in Aboriginal and non-Aboriginal otitis-prone children were assessed in comparison to non-otitis-prone children. Reduced humoral responses to rsPilA, PD and ChimV4 antigens were seen for both serumIgG and memory B-cell frequency but not non-biofilm related protein OMP26. Reduced Th17 and increased Th2 cytokine responses to PD, increased global frequencies of cytotoxic T-cells, Tfh cells,NK cells, monocytes and dendritic cells were also noted.",

keywords = "Immunology, paediatrics infectious diseases, otitis media, non-typeable Haemophilus influenzae (NTHi), Biofilms, Humoral immunity, cellular immunity, otology",

author = "Sharon Clark",

year = "2023",

doi = "10.26182/pewz-th32",

language = "English",

school = "The University of Western Australia",

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TY - BOOK

T1 - A systems immunology approach to understanding the development of otitis media in otitis prone children

AU - Clark, Sharon

PY - 2023

Y1 - 2023

N2 - Chronic otitis media (OM) affects many children particularly Australian Aboriginal children and is predominantly caused by non-typeable Haemophilus influenzae (NTHi) within biofilms. Innate and adaptive immune responses to NTHi biofilm-associated proteins rsPilA, Protein D and ChimV4 in Aboriginal and non-Aboriginal otitis-prone children were assessed in comparison to non-otitis-prone children. Reduced humoral responses to rsPilA, PD and ChimV4 antigens were seen for both serumIgG and memory B-cell frequency but not non-biofilm related protein OMP26. Reduced Th17 and increased Th2 cytokine responses to PD, increased global frequencies of cytotoxic T-cells, Tfh cells,NK cells, monocytes and dendritic cells were also noted.

AB - Chronic otitis media (OM) affects many children particularly Australian Aboriginal children and is predominantly caused by non-typeable Haemophilus influenzae (NTHi) within biofilms. Innate and adaptive immune responses to NTHi biofilm-associated proteins rsPilA, Protein D and ChimV4 in Aboriginal and non-Aboriginal otitis-prone children were assessed in comparison to non-otitis-prone children. Reduced humoral responses to rsPilA, PD and ChimV4 antigens were seen for both serumIgG and memory B-cell frequency but not non-biofilm related protein OMP26. Reduced Th17 and increased Th2 cytokine responses to PD, increased global frequencies of cytotoxic T-cells, Tfh cells,NK cells, monocytes and dendritic cells were also noted.

KW - Immunology

KW - paediatrics infectious diseases

KW - otitis media

KW - non-typeable Haemophilus influenzae (NTHi)

KW - Biofilms

KW - Humoral immunity

KW - cellular immunity

KW - otology

U2 - 10.26182/pewz-th32

DO - 10.26182/pewz-th32

M3 - Doctoral Thesis

ER -

A systems immunology approach to understanding the development of otitis media in otitis prone children

Abstract

UN SDGs

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Research output

Egg‐sensitised infants have elevated CD4+ effector memory T regulatory cells from birth

Biofilms and intracellular infection in otitis media

Australian Aboriginal Otitis-Prone Children Produce High-Quality Serum IgG to Putative Nontypeable Haemophilus influenzae Vaccine Antigens at Lower Titres Compared to Non-Aboriginal Children

Cite this